TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Wendlandt, Tim
A1  - Britz, Beate
A1  - Kleinow, Tatjana
A1  - Hipp, Katharina
A1  - Eber, Fabian
A1  - Wege, Christina
ED  - Murphy, Alex M.
ED  - Palukaitis, Peter
T1  - Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach
JF  - Viruses
N2  - This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages and presents a novel, efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses, from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. The best practices and rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls, and ‘forgotten’ or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwhile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of these multivalent soft-matter nanorods.
KW  - tobacco mosaic virus (TMV)
KW  - turnip vein clearing virus (TVCV)
KW  - plant virus
KW  - history
KW  - purification
KW  - isolation
KW  - precipitation
KW  - ultracentrifugation
KW  - density gradients
KW  - iodixanol
Y1  - 2024
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:ofb1-opus4-88433
SN  - 1999-4915
SS  - 1999-4915
U6  - https://doi.org/10.3390/v16060884
DO  - https://doi.org/10.3390/v16060884
VL  - 16
IS  - 6
SP  - 1
EP  - 42
PB  - MDPI
CY  - Basel
ER  -