@article{SeigelSchroeckHauseretal.2011,
  author    = {Andrea Seigel and Alexandra Schr{\"o}ck and Rebekka Hauser and Bernd Spangenberg},
  title     = {Sensitive quantification of diclofenac and ibuprofen using thin layer chromatography coupled with a vibrio fisheri bioluminescence assay},
  series   = {Journal of Liquid Chromatography \& Related Technologies},
  volume    = {34},
  number    = {10/11},
  publisher = {Taylor \& Francis},
  issn      = {1082-6076},
  doi       = {10.1080/10826076.2011.571149},
  pages     = {817 -- 828},
  year      = {2011},
  abstract  = {We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-camera registered the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppressed the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation. The separation method is inexpensive, fast, and reliable.},
  language  = {en}
}