TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Seigel, Andrea
A1  - Schröck, Alexandra
A1  - Hauser, Rebekka
A1  - Spangenberg, Bernd
T1  - Sensitive quantification of diclofenac and ibuprofen using thin layer chromatography coupled with a vibrio fisheri bioluminescence assay
JF  - Journal of Liquid Chromatography & Related Technologies
N2  - We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-camera registered the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppressed the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation. The separation method is inexpensive, fast, and reliable.
KW  - Dünnschichtchromatographie
KW  - Diclofenac
KW  - Ibuprofen
KW  - Inhaltsstoff
Y1  - 2011
SN  - 1082-6076
SS  - 1082-6076
U6  - https://doi.org/10.1080/10826076.2011.571149
DO  - https://doi.org/10.1080/10826076.2011.571149
VL  - 34
IS  - 10/11
SP  - 817
EP  - 828
PB  - Taylor & Francis
ER  -