@article{Spangenberg2015,
  author    = {Bernd Spangenberg},
  title     = {Two-Dimensional Thin-Layer Chromatography of Polyaromatic Hydrocarbons Measured by Absorption, Fluorescence, and Chemiluminescence},
  series   = {JPC - Journal of Planar Chromatography - Modern TLC},
  volume    = {28},
  number    = {2},
  publisher = {Akad{\´e}miai Kiad{\´o}},
  issn      = {0933-4173 (Print)},
  doi       = {10.1556/JPC.28.2015.2.9},
  pages     = {139 -- 143},
  year      = {2015},
  abstract  = {A 2D-separation of 16 polyaromatic hydrocarbons (PAHs) according to the Environmental Protecting Agency (EPA) standard was introduced. Separation took place on a TLC RP-18 plate (Merck, 1.05559). In the first direction, the plate was developed twice using n-pentane at −20°C as the mobile phase. The mixture acetonitrile-methanol-acetone-water (12:8:3:3, v/v) was used for developing the plate in the second direction. Both developments were carried out over a distance of 43 mm. Further on in this publication, a specific and very sensitive indication method for benzo[a]pyrene and perylene was presented. The method can detect these hazardous compounds even in complicated PAH mixtures. These compounds can be quantified by a simple chemiluminescent reaction with a limit of detection (LOD) of 48 pg per band for perylene and 95 pg per band for benzo[a]pyrene. Although these compounds were separated from all other PAHs in the standard, a separation of both compounds was not possible from one another. The method is suitable for tracing benzo[a]pyrene and/or perylene. The proposed chemiluminescence screening test on PAHs is extremely sensitive but may indicate a false positive result for benzo[a]pyrene.},
  language  = {en}
}