TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Spangenberg, Bernd
T1  - Two-Dimensional Thin-Layer Chromatography of Polyaromatic Hydrocarbons Measured by Absorption, Fluorescence, and Chemiluminescence
JF  - JPC - Journal of Planar Chromatography - Modern TLC
N2  - A 2D-separation of 16 polyaromatic hydrocarbons (PAHs) according to the Environmental Protecting Agency (EPA) standard was introduced. Separation took place on a TLC RP-18 plate (Merck, 1.05559). In the first direction, the plate was developed twice using n-pentane at −20°C as the mobile phase. The mixture acetonitrile-methanol-acetone-water (12:8:3:3, v/v) was used for developing the plate in the second direction. Both developments were carried out over a distance of 43 mm. Further on in this publication, a specific and very sensitive indication method for benzo[a]pyrene and perylene was presented. The method can detect these hazardous compounds even in complicated PAH mixtures. These compounds can be quantified by a simple chemiluminescent reaction with a limit of detection (LOD) of 48 pg per band for perylene and 95 pg per band for benzo[a]pyrene. Although these compounds were separated from all other PAHs in the standard, a separation of both compounds was not possible from one another. The method is suitable for tracing benzo[a]pyrene and/or perylene. The proposed chemiluminescence screening test on PAHs is extremely sensitive but may indicate a false positive result for benzo[a]pyrene.
KW  - Dünnschichtchromatographie
Y1  - 2015
SN  - 0933-4173 (Print)
SS  - 0933-4173 (Print)
SN  - 1789-0993 (Online)
SS  - 1789-0993 (Online)
U6  - https://doi.org/10.1556/JPC.28.2015.2.9
DO  - https://doi.org/10.1556/JPC.28.2015.2.9
VL  - 28
IS  - 2
SP  - 139
EP  - 143
PB  - Akadémiai Kiadó
ER  -