TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Możejko, Justyna
A1  - Wilke, Andreas
A1  - Przbylek, Grzegorz
A1  - Ciesielski, Slawomir
T1  - Mcl-PHAs produced by Pseudomonas sp. Gl01 using fed-batch cultivation with waste rapeseed oil as carbon source
JF  - Journal of Microbiology and Biotechnology: JMB
N2  - The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fedbatch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source. The biomass value reached 3.0 g/l of CDW with 20% of PHAs content within 48 h of cultivation. The polymer was purified from lyophilized cells and analyzed by gas chromatography (GC). The results revealed that the monomeric composition of the obtained polyesters depended on the available substrate. When glucose was used in the growth phase, 3-hydroxyundecanoate and 3- hydroxydodecanoate were found in the polymer composition, whereas in the PHAs-accumulating stage, the Pseudomonas Gl01 strain synthesized mcl-PHAs consisting mainly of 3- hydroxyoctanoate and 3-hydroxydecanoate. The transcriptional analysis using reverse-transcription real-time PCR reaction revealed that the phaC1 gene could be transcribed simultaneously to the phaZ gene.
KW  - Rapsöl
KW  - Kohlenstoff
Y1  - 2012
UR  - https://www.researchgate.net/publication/221982277
SN  - 1017-7825
SS  - 1017-7825
U6  - https://doi.org/10.4014/jmb.1106.06039
DO  - https://doi.org/10.4014/jmb.1106.06039
PM  - 22450793
VL  - 22
IS  - 3
SP  - 371
EP  - 377
ER  -