TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Ahrens, Björn
A1  - Blankenhorn, Dirk
A1  - Spangenberg, Bernd
T1  - Advanced fibre optical scanning in thin-layer chromatography for drug identification
JF  - Journal of Chromatography B
N2  - A systematic toxicological analysis procedure using high-performance thin layer chromatography in combination with fibre optical scanning densitometry for identification of drugs in biological samples is presented. Two examples illustrate the practicability of the technique. First, the identification of a multiple intake of analgesics: codeine, propyphenazone, tramadol, flupirtine and lidocaine, and second, the detection of the sedative diphenhydramine. In both cases, authentic urine specimens were used. The identifications were carried out by an automatic measurement and computer-based comparison of in situ UV spectra with data from a compiled library of reference spectra using the cross-correlation function. The technique allowed a parallel recording of chromatograms and in situ UV spectra in the range of 197–612 nm. Unlike the conventional densitometry, a dependency of UV spectra by concentration of substance in a range of 250–1000 ng/spot was not observed.
KW  - Dünnschichtchromatographie
KW  - Faseroptik
KW  - Dichtebestimmung
Y1  - 2002
SN  - 1873-376X
SS  - 1873-376X
U6  - https://doi.org/10.1016/S1570-0232(01)00617-1
DO  - https://doi.org/10.1016/S1570-0232(01)00617-1
VL  - 772
IS  - 1
SP  - 11
EP  - 18
PB  - Elsevier
ER  -