TY  - CHAP
U1  - Konferenzveröffentlichung
A1  - Milz, Barbara
A1  - Klein, Karl-Friedrich
A1  - Spangenberg, Bernd
T1  - Quantitative Two-Dimensional Thin-Layer Chromatography Using a Diode-Array Detector
T2  - JPC : Journal of Planar Chromatography - Modern TLC
N2  - We will present the first example of a two-dimensional scanned TLC-plate, measured by use of a diode-array scanner. A spatial resolution of 250 µm was achieved on plate. The system provides real 2D fluorescence and absorption spectra in the wavelength-range from 190 to 1000 nm with a spectral resolution of greater than 1 nm. A mixture of 12 sulphonamides was separated by using a cyanopropyl-coated silica gel plate (Merck, 1.16464) with the solvent mix of methyl tert-butyl ether-methanol-dichloromethane-cyclohexane-NH3 (25%) (48:2:2:1:1, v/v) in the first and with a mixture of water-acetonitrile-dioxane-ethanol (8:2:1:1, v/v) in the second direction. Both developments were carried out over a distance of 70 mm. A separation number (spot capacity) of 259 was calculated. We discussed a new formula for its calculation in 2D-TLC separations. The drawback of this method is that measuring a 2D-TLC plate needs more than 3 h measurement time.
KW  - HPTLC
KW  - TLC
KW  - Sulphonamides
KW  - 2D-TLC
KW  - Diode-array detection
Y1  - 2012
SN  - 0933-4173 (Print)
SS  - 0933-4173 (Print)
SN  - 1789-0993 (Online)
SS  - 1789-0993 (Online)
U6  - https://doi.org/10.1556/JPC.25.2012.6.1
DO  - https://doi.org/10.1556/JPC.25.2012.6.1
VL  - 25
IS  - 6
SP  - 493
EP  - 497
PB  - Akadémiai Kiadó
ER  -