@inproceedings{SpangenbergAhrensKlein2001, author = {Bernd Spangenberg and Bj{\"o}rn Ahrens and Karl-Friedrich Klein}, title = {TLC-Analysis in forensic sciences using a diode-array detector}, series = {Chromatographia : An International Journal for Separation Science}, volume = {53}, number = {Suppl. 1}, publisher = {Vieweg}, issn = {0009-5893}, doi = {10.1007/BF02490372}, pages = {S438 -- S441}, year = {2001}, abstract = {HPTLC (High Performance Thin Layer Chromatography) is a well known and versatile separation method which shows many advantages when compared to other separation techniques. The method is fast and inexpensive and does not need time-consuming pretreatments. For visualisation of the sample distribution on a HPTLC-plate we developed a new and sturdy HPTLC-scanner. The scanner allows simultaneous registrations of spectra in a range from 198 nm to 612 nm with a spectral resolution of better than 0.8 nm. The on-plate spatial resolution is better than 160 μm. The measurement of 450 spectra in one separation track does not need more than two minutes. The new diode-array scanner offers a fast survey over a TLC-separation and makes various chemometric applications possible. For compound identification a cross-correlation function is described to compare UV sample spectra with appropriate library data. The cross-correlation function herein described can also be used for purity testing. Unresolved peaks can be virtually separated by use of a least squares fit algorithm. In summary, the diode arry system delivers much more information than the commonly used TLC-scanner.}, language = {en} }