TY  - CHAP
U1  - Konferenzveröffentlichung
A1  - Spangenberg, Bernd
A1  - Ahrens, Björn
A1  - Klein, Karl-Friedrich
T1  - TLC-Analysis in forensic sciences using a diode-array detector
T2  - Chromatographia : An International Journal for Separation Science
N2  - HPTLC (High Performance Thin Layer Chromatography) is a well known and versatile separation method which shows many advantages when compared to other separation techniques. The method is fast and inexpensive and does not need time-consuming pretreatments. For visualisation of the sample distribution on a HPTLC-plate we developed a new and sturdy HPTLC-scanner. The scanner allows simultaneous registrations of spectra in a range from 198 nm to 612 nm with a spectral resolution of better than 0.8 nm. The on-plate spatial resolution is better than 160 μm. The measurement of 450 spectra in one separation track does not need more than two minutes. The new diode-array scanner offers a fast survey over a TLC-separation and makes various chemometric applications possible. For compound identification a cross-correlation function is described to compare UV sample spectra with appropriate library data. The cross-correlation function herein described can also be used for purity testing. Unresolved peaks can be virtually separated by use of a least squares fit algorithm. In summary, the diode arry system delivers much more information than the commonly used TLC-scanner.
KW  - Dünnschichtchromatographie
KW  - Gerichtliche Wissenschaften
KW  - Scanner
KW  - Spektrum
Y1  - 2001
SN  - 0009-5893
SS  - 0009-5893
U6  - https://doi.org/10.1007/BF02490372
DO  - https://doi.org/10.1007/BF02490372
VL  - 53
IS  - Suppl. 1
SP  - S438
EP  - S441
PB  - Vieweg
ER  -