TY  - JOUR
U1  - Zeitschriftenartikel, nicht wissenschaftlich
A1  - Spangenberg, Bernd
A1  - Seigel, Andrea
A1  - Schröck, Alexandra
A1  - Hauser, Rebekka
T1  - Quantification of Diclofenac and Ibuprofen by a Vibrio Fischeri Bioluminescence Assay
JF  - Beiträge aus Forschung & Technik
N2  - We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95+5+40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using vibrio fischeri bacteria. Within 10 minutes a CCD-camera registers the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppress the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels [from Santa Barbara Instrument Group, Inc., Santa Barbara, USA]. The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation [1]. The separation method is inexpensive, fast and reliable. Ibuprofen is named after its chemical description: iso-butyl-propanoic phenolic acid. Both pain killers are world-widein use and both substances are stable in aqueous solution. Both substances are mainly excreted in the urine.
KW  - Diclofenac
KW  - Ibuprofen
KW  - Environment
KW  - CCD-array
KW  - wastewater
KW  - human health
KW  - Vibrio Fischeri Bioluminescence Assay
Y1  - 2011
U6  - https://nbn-resolving.org/urn:nbn:de:bsz:ofb1-opus-1445
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:ofb1-opus-1445
SN  - 1866-9352
SS  - 1866-9352
VL  - 2011
SP  - 55
EP  - 56
ER  -