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New components for fiber-optic thin-layer chromatography including fluorescence

  • In thin-layer chromatography, fiber-bundle arrays have been introduced for spectral absorption measurements in the UV-region. Using all-silica fiber bundles, the exciting light will be detected after re-emission on the plate with a fiberoptic spectrometer. In addition, fluorescence light can be detected which will be masked by the re-emitted light. Therefore, it is helpful to separate theIn thin-layer chromatography, fiber-bundle arrays have been introduced for spectral absorption measurements in the UV-region. Using all-silica fiber bundles, the exciting light will be detected after re-emission on the plate with a fiberoptic spectrometer. In addition, fluorescence light can be detected which will be masked by the re-emitted light. Therefore, it is helpful to separate the absorption and fluorescence on the TLC-plate. A modified three-array assembly has been developed: using one array for detection, the two others are used for excitation with broadband band deuterium-light and with UV-LEDs adjusted to the substances under test. As an example, the quantification of glucosamine in nutritional supplements or spinach leaf extract will be described. Using simply heating of the amino-plate for derivation, the reaction product of Glucosamine can be detected sensitively either by light absorption or by fluorescence, using the new fiber-optic assembly. In addition, the properties of the new 3-row fiber-optic array and the commercially available UV-LEDs will be shown, in the interesting wavelength region for excitation of fluorescence, from 260 nm to 360 nm. The squint angle having an influence on coupling efficiency and spatial resolution will be measured with the inverse farfield method. Some properties of UV-LEDs for analytical applications will be described and discussed, too.show moreshow less

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Metadaten
Document Type:Conference Proceeding
Conference Type:Konferenzartikel
Zitierlink: https://opus.hs-offenburg.de/8516
Bibliografische Angaben
Title (English):New components for fiber-optic thin-layer chromatography including fluorescence
Conference:SPIE BiOS (20-25 January 2007 : San Jose, California, United States)
Author:Michaela BleichertStaff Member, Hanns Simon Eckhardt, Karl-Friedrich Klein, Bernd SpangenbergStaff MemberORCiDGND, Georg Hillrichs, Joachim Mannhardt
Year of Publication:2007
Creating Corporation:Society of Photo-Optical Instrumentation Engineers
Place of publication:Bellingham, Washington
Publisher:SPIE
Page Number:10
Article Number:64330F
Parent Title (English):Proceedings of SPIE
Editor:Israel Gannot
Volume:6433
ISBN:9780819465467
ISSN:1605-7422
DOI:https://doi.org/10.1117/12.717579
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
Formale Angaben
Open Access: Closed 
Licence (German):License LogoUrheberrechtlich geschützt