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Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

  • This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages and presents a novel, efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses, from the search for the causative agents of mosaicThis article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages and presents a novel, efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses, from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. The best practices and rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls, and ‘forgotten’ or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwhile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of these multivalent soft-matter nanorods.show moreshow less

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Metadaten
Document Type:Article (reviewed)
Zitierlink: https://opus.hs-offenburg.de/8843
Bibliografische Angaben
Title (English):Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach
Author:Tim Wendlandt, Beate Britz, Tatjana KleinowORCiD, Katharina Hipp, Fabian EberStaff MemberGND, Christina WegeORCiD
Year of Publication:2024
Date of first Publication:2024/05/30
Place of publication:Basel
Publisher:MDPI
First Page:1
Last Page:42
Article Number:884
Parent Title (English):Viruses
Editor:Alex M. Murphy, Peter Palukaitis
Volume:16
Issue:6
ISSN:1999-4915
DOI:https://doi.org/10.3390/v16060884
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
Tag:density gradients; history; iodixanol; isolation; plant virus; precipitation; purification; tobacco mosaic virus (TMV); turnip vein clearing virus (TVCV); ultracentrifugation
Formale Angaben
Relevance:Wiss. Zeitschriftenartikel reviewed: Listung in Master Journal List
Open Access: Open Access 
 Gold 
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International