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Quantification of astaxanthin in salmons by chemiluminescence and absorption after TLC separation

  • Astaxanthin is a keto-carotenoid, belongs to the chemical class of terpenes and is a yellow lipid soluble compound. The compound is present in marine animals like salmons and crustacean. Its colour is due to conjugated double bonds and these double bonds are responsible for its antioxidant effect. Its antioxidant activity is ten times stronger than other carotenoids and nearly 500 fold strongerAstaxanthin is a keto-carotenoid, belongs to the chemical class of terpenes and is a yellow lipid soluble compound. The compound is present in marine animals like salmons and crustacean. Its colour is due to conjugated double bonds and these double bonds are responsible for its antioxidant effect. Its antioxidant activity is ten times stronger than other carotenoids and nearly 500 fold stronger than vitamin-E. We present a new thin layer chromatography (TLC) method to measure astaxanthin on TLC-plates (Merck, 1.05554) in the visible absorption range as well as by using chemiluminescence. For separation a solvent mixture of cyclohexane and acetone (10 + 2.4, v/v) was used. The RF-value of astaxanthin is 0.14.The limit of detection in vis-absorption is 64 ng / band and the limit of quantification is 92 ng/band. In chemiluminescence the values are 90 ng / band and 115 ng/band. The method offers two independently working measurement modes on a single plate which increase the accuracy of the quantification.show moreshow less

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Metadaten
Author:Barbara MilzGND, Yvonne Anna Minar, Bernd SpangenbergGND
Publisher:Taylor & Francis
Year of Publication:2018
Language:English
Tag:Astaxanthin; Canthaxanthin; Chemiluminescence; HPTLC; Lutein; Salmon; TLC
Parent Title (English):Journal of Liquid Chromatography & Related Technologies
Volume:41
Issue:6
ISSN:1082-6076 (Print)
ISSN:1520-572X (Online)
First Page:358
Last Page:363
Document Type:Article (reviewed)
Institutes:Bibliografie
Open Access:Zugriffsbeschränkt
Release Date:2021/03/31
Licence (German):License LogoEs gilt das UrhG
URL:https://www.tandfonline.com/doi/full/10.1080/10826076.2018.1449057
DOI:https://doi.org/10.1080/10826076.2018.1449057