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Fast hyperlayer separation development in sedimentation field flow fractionation

  • Specific prototypes of sedimentation field flow fractionation devices (SdFFF) have been developed with relative success for cell sorting. However, no data are available to compare these apparatus with commercial ones. In order to compare with other devices mainly used for non-biological species, biocompatible systems were used for standard particle (latex: 3–10 μm of different size dispersities)Specific prototypes of sedimentation field flow fractionation devices (SdFFF) have been developed with relative success for cell sorting. However, no data are available to compare these apparatus with commercial ones. In order to compare with other devices mainly used for non-biological species, biocompatible systems were used for standard particle (latex: 3–10 μm of different size dispersities) separation development. In order to enhance size dependent separations, channels of reduced thickness were used (80 and 100 μm) and channel/carrier-phase equilibration procedures were necessary. For sample injection, the use of inlet tubing linked to the FFF accumulation wall, common for cell sorting, can be extended to latex species when they are eluted in the Steric Hyperlayer elution mode. It avoids any primary relaxation steps (stop flow injection procedure) simplifying series of elution processing. Mixtures composed of four different monodispersed latex beads can be eluted in 6 min with 100 μm channel thickness.show moreshow less

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Metadaten
Document Type:Article (unreviewed)
Zitierlink: https://opus.hs-offenburg.de/634
Bibliografische Angaben
Title (English):Fast hyperlayer separation development in sedimentation field flow fractionation
Author:James R. KassabStaff Member, Philippe J.P. Cardot, Richard A. ZahoranskyStaff MemberGND, Serge Battua
Year of Publication:2005
Publisher:Elsevier
First Page:8
Last Page:16
Parent Title (English):Journal of Chromatography B
Volume:826
Issue:1-2
ISSN:0378-4347
DOI:https://doi.org/10.1016/j.jchromb.2005.06.020
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
Formale Angaben
Open Access: Closed Access 
Licence (German):License LogoUrheberrechtlich geschützt