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Sensitive quantification of diclofenac and ibuprofen using thin layer chromatography coupled with a vibrio fisheri bioluminescence assay

  • We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-cameraWe present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-camera registered the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppressed the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation. The separation method is inexpensive, fast, and reliable.show moreshow less

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Metadaten
Author:Andrea Seigel, Alexandra Schröck, Rebekka Hauser, Bernd SpangenbergGND
Publisher:Taylor & Francis
Date of Publication (online):2011/05/17
Language:English
GND Keyword:Diclofenac; Dünnschichtchromatographie; Ibuprofen; Inhaltsstoff
Parent Title (English):Journal of Liquid Chromatography & Related Technologies
Volume:34
Issue:10/11
ISSN:1082-6076
First Page:817
Last Page:828
Document Type:Article (reviewed)
Institutes:Hochschule Offenburg / Bibliografie
Acces Right:Zugriffsbeschränkt
Release Date:2019/10/24
Licence (German):License LogoEs gilt das UrhG
DOI:https://doi.org/10.1080/10826076.2011.571149