A simple Method for quantifying Triazine Herbicides using Thin-Layer Chromatography and a CCD-Camera
- We present a video-densitometric quantification method for the triazine herbicides atraton, terbumeton, simazine, atrazine, and terbutylazine. Triazine herbicides were separated on silica gel using methyl-t-butyl ether, cyclohexane (1 + 1, v/v) as mobile phase. The quantification is based on a derivation reaction using chlorine and starch-iodine which forms red-brown triazine zones. MeasurementsWe present a video-densitometric quantification method for the triazine herbicides atraton, terbumeton, simazine, atrazine, and terbutylazine. Triazine herbicides were separated on silica gel using methyl-t-butyl ether, cyclohexane (1 + 1, v/v) as mobile phase. The quantification is based on a derivation reaction using chlorine and starch-iodine which forms red-brown triazine zones. Measurements were carried out using a 16 bit ST-1603ME CCD camera with 1.56 megapixel from Santa Barbara Instrument Group, Inc., Santa Barbara, USA. A white LED was used for illumination purposes. The range of linearity covers two magnitudes using the (1/R-1) expression data transformation. The signal-to-noise ratio increases directly linearly with the measurement time. The separation method is cheap, fast and reliable.…
Document Type: | Contribution to a Periodical |
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Zitierlink: | https://opus.hs-offenburg.de/47 | Bibliografische Angaben |
Title (English): | A simple Method for quantifying Triazine Herbicides using Thin-Layer Chromatography and a CCD-Camera |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Melanie BroszatStaff MemberGND, Helena Ernst |
Date of Publication (online): | 2011/03/21 |
Publisher: | Taylor & Francis |
First Page: | 948 |
Last Page: | 956 |
Parent Title (English): | Journal of Liquid Chromatography & Related Technologies |
Volume: | 33 |
Issue: | 7/8 |
ISSN: | 1082-6076 |
DOI: | https://doi.org/10.1080/10826071003766245 |
URN: | https://urn:nbn:de:bsz:ofb1-opus-461 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Journals: | Beiträge aus Forschung & Technik |
DDC classes: | 500 Naturwissenschaften und Mathematik / 540 Chemie |
GND Keyword: | Chromatographie; Dünnschichtchromatographie; Herbizid; Triazine | Formale Angaben |
Open Access: | Open Access |
Licence (German): | Veröffentlichungsvertrag für Publikationen mit Print on Demand |