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New approaches in TLC-densitometry

  • The use of a TLC scanner can be regarded as a key step in high performance thin layer chromatography (HPTLC). Densitometric measurements transform the substance distribution on a TLC plate into digital computer data. Systems that allow quantitative measurements have been available for many years for either fluorescence or ultraviolet absorption measurements, while lately the reflection analysisThe use of a TLC scanner can be regarded as a key step in high performance thin layer chromatography (HPTLC). Densitometric measurements transform the substance distribution on a TLC plate into digital computer data. Systems that allow quantitative measurements have been available for many years for either fluorescence or ultraviolet absorption measurements, while lately the reflection analysis mode for both types is the most common application. New scanning approaches are designed to aid the analyst who has common demands for TLC-densitometry without using special data, such as scanned images. Two examples that have been developed lately in the laboratories of the authors are described in this paper. These approaches were developed on the basis of current needs for analysts who employ TLC as a tool in research, as well as in routine analysis. One approach is aimed to support analysts in economically disadvantaged areas, where cost intensive apparatus is unsuitable but trace analysis by simple means is required. The other system, allows the spectral determination of chromatographic spots on TLC plates covering the ultraviolet and visible range, thus, revealing highly desired information for the analyst.show moreshow less

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Metadaten
Document Type:Article (reviewed)
Zitierlink: https://opus.hs-offenburg.de/3486
Bibliografische Angaben
Title (English):New approaches in TLC-densitometry
Author:Jörg Stroka, Bernd SpangenbergStaff MemberORCiDGND, Elke Anklam
Year of Publication:2002
Publisher:Taylor & Francis
First Page:1497
Last Page:1513
Parent Title (English):Journal of Liquid Chromatography & Related Technologies
Volume:25
Issue:10/11
ISSN:1520-572X
DOI:https://doi.org/10.1081/JLC-120005700
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
GND Keyword:Densitometrie; Dünnschichtchromatographie; Messtechnik
Formale Angaben
Open Access: Closed Access 
Licence (German):License LogoUrheberrechtlich geschützt