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A Simple and Reliable HPTLC Method for the Quantification of the Intense Sweetener Sucralose®

  • This paper describes a simple and fast thin layer chromatography (TLC) method for the monitoring of the relatively new intense sweetener Sucralose® in various food matrices. The method requires little or no sample preparation to isolate or concentrate the analyte. The Sucralose® extract is separated on amino‐TLC‐plates, and the analyte is derivatized “reagent‐free” by heating the developed plateThis paper describes a simple and fast thin layer chromatography (TLC) method for the monitoring of the relatively new intense sweetener Sucralose® in various food matrices. The method requires little or no sample preparation to isolate or concentrate the analyte. The Sucralose® extract is separated on amino‐TLC‐plates, and the analyte is derivatized “reagent‐free” by heating the developed plate for 20 min at 190°C. Spots can be measured either in the absorption or fluorescence mode. The method allows the determination of Sucralose® at the levels of interest regarding foreseen European legislation (>50 mg/kg) with excellent repeatability (RSD = 3.4%) and recovery data (95%).show moreshow less

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Metadaten
Document Type:Article (reviewed)
Zitierlink: https://opus.hs-offenburg.de/4740
Bibliografische Angaben
Title (English):A Simple and Reliable HPTLC Method for the Quantification of the Intense Sweetener Sucralose®
Author:Bernd SpangenbergStaff MemberORCiDGND, Jörg Stroka, Isabel Arranz, Elke Anklam
Year of Publication:2003
Publisher:Taylor & Francis
First Page:2729
Last Page:2739
Parent Title (English):Journal of Liquid Chromatography & Related Technologies
Volume:26
Issue:16
ISSN:1082-6076 (Print)
DOI:https://doi.org/10.1081/JLC-120024543
URL:https://www.tandfonline.com/doi/full/10.1081/JLC-120024543
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
Tag:Diode‐array TLC; Fluorescence detection; Fluorescence enhancement; HPTLC; Intense sweetener; Reagent‐free derivatization; Sucralose
Formale Angaben
Open Access: Closed Access 
Licence (German):License LogoUrheberrechtlich geschützt