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Optimized Determination of Caffeine, Equol, and Artemisinin by High-Performance Thin-Layer Chromatography–Direct Analysis in Real Time–Time of Flight–Mass Spectrometry.

  • We present a planar chromatographic separation method for the compounds caffeine, artemisinin, and equol, separated on high-performance thin-layer chromatography (HPTLC) silica gel plates. As solvents for separation, methyl t-butyl ether and cyclohexane (1:1, V/V) have been used for equol, cyclohexane and ethyl acetate (7:3, V/V) for artemisinin, and ethyl acetate and acetone (7:3, V/V) forWe present a planar chromatographic separation method for the compounds caffeine, artemisinin, and equol, separated on high-performance thin-layer chromatography (HPTLC) silica gel plates. As solvents for separation, methyl t-butyl ether and cyclohexane (1:1, V/V) have been used for equol, cyclohexane and ethyl acetate (7:3, V/V) for artemisinin, and ethyl acetate and acetone (7:3, V/V) for caffeine. After separation, the plate was scanned with a very specific time of flight-direct analysis in real time-mass spectrometry (TOF-DART-MS) system using the (M + 1)+ signals of equol, artemisinin, and caffeine. The (M + 1) peak of artemisinin at 283.13 m/z is clearly detectable, which is the proof that DART-MS is applicable for the quantitative determination of rather instable molecules. The planar set-up of DART source, HPTLC plate and detector inlet in a line showed higher sensitivities compared to desorption at an angle. The optimal detector voltage increases with the molar mass of the analyte, thus an individual determination of optimal detector voltage setting for the different analyte is recommended to achieve the best possible measurement conditions. In conclusion, DART-MS detection in combination with an HPTLC separation allows very specific quantification of all three compounds.show moreshow less

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Metadaten
Author:Fabian Eichner, Bernd SpangenbergGND
Year of Publication:2019
Language:English
Tag:Artemisinin; Caffeine; Direct analysis in real time–mass spectrometry; Equol; High-performance thin-layer chromatography
DDC classes:500 Naturwissenschaften und Mathematik
Parent Title (English):JPC - Journal of Planar Chromatography - Modern TLC
Volume:32
Issue:3
ISSN:1789-0993
First Page:197
Last Page:203
Document Type:Article (reviewed)
Institutes:Bibliografie
Release Date:2020/01/17
Licence (German):License LogoEs gilt das UrhG
DOI:https://doi.org/10.1556/1006.2019.32.3.4