A simple and reliable method for quantification of glucosamine in nutritional supplements
- HPTLC on amino plates, with simple heating of the plates for derivatization, has been used for quantification of glucosamine in nutritional supplements. On heating the plate glucosamine reacts to form a compound which strongly absorbs light between 305 and 330 nm, with weak fluorescence. The reaction product can be detected sensitively either by absorption of light or by fluorescence detection.HPTLC on amino plates, with simple heating of the plates for derivatization, has been used for quantification of glucosamine in nutritional supplements. On heating the plate glucosamine reacts to form a compound which strongly absorbs light between 305 and 330 nm, with weak fluorescence. The reaction product can be detected sensitively either by absorption of light or by fluorescence detection. The detection limit in absorption mode is approximately 25 ng per spot. In fluorescence mode a detection limit of 15 ng is achievable. A calibration plot for absorption detection is linear in the range 25 to 4000 ng glucosamine. The derivative formed from glucosamine by heating is stable for months, and the relative standard deviation is 1.64% for 600 ng glucosamine. The amounts of glucosamine found in nutritional supplements were in agreement with the label declarations.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/3502 | Bibliografische Angaben |
Title (English): | A simple and reliable method for quantification of glucosamine in nutritional supplements |
Author: | Michaela Bleichert, Hanns Simon Eckhardt, Karl-Friedrich Klein, Bernd SpangenbergStaff MemberORCiDGND |
Year of Publication: | 2008 |
Publisher: | Akadémiai Kiadó |
First Page: | 55 |
Last Page: | 59 |
Parent Title (English): | JPC - Journal of Planar Chromatography - Modern TLC |
Volume: | 21 |
Issue: | 1 |
ISSN: | 0933-4173 |
DOI: | https://doi.org/10.1556/JPC.2007.6005 |
URL: | https://www.researchgate.net/publication/245405290 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
GND Keyword: | Ergänzung; Glucosamin; Nahrung | Formale Angaben |
Open Access: | Open Access |
Licence (German): | Urheberrechtlich geschützt |