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Quantitative Two-Dimensional Thin-Layer Chromatography Using a Diode-Array Detector

  • We will present the first example of a two-dimensional scanned TLC-plate, measured by use of a diode-array scanner. A spatial resolution of 250 µm was achieved on plate. The system provides real 2D fluorescence and absorption spectra in the wavelength-range from 190 to 1000 nm with a spectral resolution of greater than 1 nm. A mixture of 12 sulphonamides was separated by using a cyanopropyl-coatedWe will present the first example of a two-dimensional scanned TLC-plate, measured by use of a diode-array scanner. A spatial resolution of 250 µm was achieved on plate. The system provides real 2D fluorescence and absorption spectra in the wavelength-range from 190 to 1000 nm with a spectral resolution of greater than 1 nm. A mixture of 12 sulphonamides was separated by using a cyanopropyl-coated silica gel plate (Merck, 1.16464) with the solvent mix of methyl tert-butyl ether-methanol-dichloromethane-cyclohexane-NH3 (25%) (48:2:2:1:1, v/v) in the first and with a mixture of water-acetonitrile-dioxane-ethanol (8:2:1:1, v/v) in the second direction. Both developments were carried out over a distance of 70 mm. A separation number (spot capacity) of 259 was calculated. We discussed a new formula for its calculation in 2D-TLC separations. The drawback of this method is that measuring a 2D-TLC plate needs more than 3 h measurement time.show moreshow less

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Metadaten
Document Type:Conference Proceeding
Conference Type:Konferenzartikel
Zitierlink: https://opus.hs-offenburg.de/7287
Bibliografische Angaben
Title (English):Quantitative Two-Dimensional Thin-Layer Chromatography Using a Diode-Array Detector
Conference:International Symposium on Separation Sciences (17. : September 5-9, 2011 : Cluj-Napoca, Romania)
Author:Barbara MilzStaff MemberGND, Karl-Friedrich Klein, Bernd SpangenbergStaff MemberORCiDGND
Year of Publication:2012
Publisher:Akadémiai Kiadó
First Page:493
Last Page:497
Parent Title (English):JPC : Journal of Planar Chromatography - Modern TLC
Volume:25
Issue:6
ISSN:0933-4173 (Print)
ISSN:1789-0993 (Online)
DOI:https://doi.org/10.1556/JPC.25.2012.6.1
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
Tag:2D-TLC; Diode-array detection; HPTLC; Sulphonamides; TLC
Formale Angaben
Open Access: Closed 
Licence (German):License LogoUrheberrechtlich geschützt