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Enzymatic Pretreatment Optimization to Increase Biogas Production

  • As the population grows, so does the amount of biowaste. As demand for energy grows, biogas is a promising solution to the problem. Lignocellulosic materials are challenged of slow degradability due to the presence of polymers such as cellulose, lignin and hemicellulose. There are several pretreatment methods available to enhance the degradability of such materials, including enzymaticAs the population grows, so does the amount of biowaste. As demand for energy grows, biogas is a promising solution to the problem. Lignocellulosic materials are challenged of slow degradability due to the presence of polymers such as cellulose, lignin and hemicellulose. There are several pretreatment methods available to enhance the degradability of such materials, including enzymatic pretreatment. In this pretreatment, there are few parameters that can influence the results, the most important being the enzyme to solid ratio and the solid to liquid ratio. During this project, experiments were conducted to determine the optimal conditions for those two factors. It was discovered that a solid to liquid ratio of 31 g of buffer per 1 gram of organic dry matter produced the highest reducing sugar release in flasks when combined with 34 mg of protein per 1 gram of organic dry mass. Additionally, another experiment was carried out to investigate the impact of enzymatic pretreatment on biogas production using artificial biowaste as a substrate. Artificial biowaste produced 577,9 NL/kg oDM, while enzymatically pretreated biowaste produced 639,3 NL/kg oDM. This resulted in a 10,6% rise in cumulative biogas production compared to its use without enzymatic pretreatment. By the conclusion of the investigation, specific cumulative dry methane yields of 364,7 NL/kg oDM and 426,3 NL/kg oDM were obtained from artificial biowaste without and with enzymatic pretreatment, respectively. This resulted in a methane production boost of 16,9%. Additionally in case of the reactors with enzymatically pretreated substrate kinetic constant was lower more than double, where maximum volume of biogas increased, comparing to the reactors without enzymatic pretreatment.show moreshow less

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Metadaten
Document Type:Master's Thesis
Zitierlink: https://opus.hs-offenburg.de/8058
Bibliografische Angaben
Title (English):Enzymatic Pretreatment Optimization to Increase Biogas Production
Author:Michał Chróst
Advisor:Andreas Wilke, Fabian Haitz
Year of Publication:2023
Publishing Institution:Hochschule Offenburg
Granting Institution:Hochschule Offenburg
Page Number:57
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Abschlussarbeiten / Master-Studiengänge / MPE
DDC classes:600 Technik, Medizin, angewandte Wissenschaften
GND Keyword:Biogas; Enzym; Organischer Abfall; Verfahrenstechnik
Tag:biogas; biowaste; enzymatic pretreatment; lignocellulosic material; optimization
Formale Angaben
Open Access: Closed Access 
Licence (German):License LogoUrheberrechtlich geschützt