Development of Technical Substrate for Cellulase Production using Trichoderma reesei
- The cellulase-producing Trichoderma reesei strain RL-P37 exhibits significant potential, yielding 7.3 g/L of cellulase in 241 hours. Microscopic investigations reveal a link between spore formation and enzyme production, suggesting the need for research into the intricate relationship between enzyme production, stress responses, and the nutritional prerequisites of fungi. Comparatively, the use ofThe cellulase-producing Trichoderma reesei strain RL-P37 exhibits significant potential, yielding 7.3 g/L of cellulase in 241 hours. Microscopic investigations reveal a link between spore formation and enzyme production, suggesting the need for research into the intricate relationship between enzyme production, stress responses, and the nutritional prerequisites of fungi. Comparatively, the use of sodium hydroxide (NaOH) treatment, as opposed to water treatment, results in the reduction of micronutrient content and carbon source extraction as filtrate. Despite these challenges, research by He et al. (2021) highlights NaOH's efficiency in cellulose extraction from plant-based sources. Using NaOH pretreatment can be proven as effective by designing a proper cultivation method. The selection of inducers for enzyme induction gains importance, with soluble inducers, as emphasized by Zhang et al. (2022), exhibiting superior effectiveness. Hence, adopting soluble inducers in designing cultivation methods for improved enzyme production in shaking flasks is recommended. Enzymatic treatment of bio-waste, as outlined by Hu et al. (2021), shows promise in augmenting essential component content by breaking down plant cell walls and intercellular compartments. However, the feasibility of using an artificial bio-waste medium for cultivating Trichoderma reesei is questioned. Investigating the impact of micronutrient levels, particularly the inhibitory role of zinc, on fungal growth becomes essential. These findings underscore the necessity for ongoing research and optimization in cellulase production, emphasizing both strain productivity and cultivation methodologies.…
Document Type: | Master's Thesis |
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Zitierlink: | https://opus.hs-offenburg.de/7955 | Bibliografische Angaben |
Title (English): | Development of Technical Substrate for Cellulase Production using Trichoderma reesei |
Author: | Olga Zielińska |
Advisor: | Andreas Wilke, Thomas Eisele |
Year of Publication: | 2023 |
Granting Institution: | Hochschule Offenburg |
Page Number: | VII, 64 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Abschlussarbeiten / Bachelor-Studiengänge / BT |
DDC classes: | 600 Technik, Medizin, angewandte Wissenschaften / 660 Technische Chemie / 660 Chemische Verfahrenstechnik |
GND Keyword: | Cellulasen; Fermentation; Organischer Abfall; Trichoderma reesei |
Tag: | biowaste; cellulase; fermentation | Formale Angaben |
Open Access: | Closed |
Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |