TLC-Analysis in forensic sciences using a diode-array detector
- HPTLC (High Performance Thin Layer Chromatography) is a well known and versatile separation method which shows many advantages when compared to other separation techniques. The method is fast and inexpensive and does not need time-consuming pretreatments. For visualisation of the sample distribution on a HPTLC-plate we developed a new and sturdy HPTLC-scanner. The scanner allows simultaneousHPTLC (High Performance Thin Layer Chromatography) is a well known and versatile separation method which shows many advantages when compared to other separation techniques. The method is fast and inexpensive and does not need time-consuming pretreatments. For visualisation of the sample distribution on a HPTLC-plate we developed a new and sturdy HPTLC-scanner. The scanner allows simultaneous registrations of spectra in a range from 198 nm to 612 nm with a spectral resolution of better than 0.8 nm. The on-plate spatial resolution is better than 160 μm. The measurement of 450 spectra in one separation track does not need more than two minutes. The new diode-array scanner offers a fast survey over a TLC-separation and makes various chemometric applications possible. For compound identification a cross-correlation function is described to compare UV sample spectra with appropriate library data. The cross-correlation function herein described can also be used for purity testing. Unresolved peaks can be virtually separated by use of a least squares fit algorithm. In summary, the diode arry system delivers much more information than the commonly used TLC-scanner.…
Document Type: | Conference Proceeding |
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Conference Type: | Konferenzartikel |
Zitierlink: | https://opus.hs-offenburg.de/3478 | Bibliografische Angaben |
Title (English): | TLC-Analysis in forensic sciences using a diode-array detector |
Conference: | International Symposium on Chromatography (23. : October 1-5, 2000 : London, UK) |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Björn Ahrens, Karl-Friedrich Klein |
Year of Publication: | 2001 |
Publisher: | Vieweg |
First Page: | S438 |
Last Page: | S441 |
Parent Title (English): | Chromatographia : An International Journal for Separation Science |
Volume: | 53 |
Issue: | Suppl. 1 |
ISSN: | 0009-5893 |
DOI: | https://doi.org/10.1007/BF02490372 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
GND Keyword: | Dünnschichtchromatographie; Gerichtliche Wissenschaften; Scanner; Spektrum | Formale Angaben |
Open Access: | Closed Access |
Licence (German): | Urheberrechtlich geschützt |