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Quantification of Diclofenac and Ibuprofen by a Vibrio Fischeri Bioluminescence Assay

  • We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95+5+40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using vibrio fischeri bacteria. Within 10 minutes a CCD-cameraWe present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95+5+40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using vibrio fischeri bacteria. Within 10 minutes a CCD-camera registers the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppress the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels [from Santa Barbara Instrument Group, Inc., Santa Barbara, USA]. The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation [1]. The separation method is inexpensive, fast and reliable. Ibuprofen is named after its chemical description: iso-butyl-propanoic phenolic acid. Both pain killers are world-widein use and both substances are stable in aqueous solution. Both substances are mainly excreted in the urine.show moreshow less

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Metadaten
Author:Bernd SpangenbergGND, Andrea Seigel, Alexandra Schröck, Rebekka Hauser
Date of Publication (online):2012/07/11
Language:English
GND Keyword:Diclofenac; Environment; Ibuprofen
Tag:CCD-array; Vibrio Fischeri Bioluminescence Assay; human health; wastewater
DDC classes:500 Naturwissenschaften und Mathematik / 540 Chemie
Parent Title (German):Beiträge aus Forschung & Technik
Volume:2011
ISSN:1866-9352
First Page:55
Last Page:56
Document Type:Contribution to a Periodical
Open Access:Frei zugänglich
Institutes:Bibliografie
Journals:Beiträge aus Forschung & Technik
Release Date:2012/07/11
Licence (German):License LogoVeröffentlichungsvertrag für Publikationen mit Print on Demand
URN:urn:nbn:de:bsz:ofb1-opus-1445