Volltext-Downloads (blau) und Frontdoor-Views (grau)
  • search hit 2 of 2
Back to Result List

Sensitive quantification of diclofenac and ibuprofen using thin layer chromatography coupled with a vibrio fisheri bioluminescence assay

  • We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-cameraWe present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-camera registered the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppressed the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation. The separation method is inexpensive, fast, and reliable.show moreshow less

Export metadata

Additional Services

Search Google Scholar

Statistics

frontdoor_oas
Metadaten
Document Type:Article (reviewed)
Zitierlink: https://opus.hs-offenburg.de/3536
Bibliografische Angaben
Title (English):Sensitive quantification of diclofenac and ibuprofen using thin layer chromatography coupled with a vibrio fisheri bioluminescence assay
Author:Andrea SeigelStaff Member, Alexandra Schröck, Rebekka Hauser, Bernd SpangenbergStaff MemberORCiDGND
Date of Publication (online):2011/05/17
Publisher:Taylor & Francis
First Page:817
Last Page:828
Parent Title (English):Journal of Liquid Chromatography & Related Technologies
Volume:34
Issue:10/11
ISSN:1082-6076
DOI:https://doi.org/10.1080/10826076.2011.571149
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
GND Keyword:Diclofenac; Dünnschichtchromatographie; Ibuprofen; Inhaltsstoff
Formale Angaben
Open Access: Closed Access 
Licence (German):License LogoUrheberrechtlich geschützt