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Two-Dimensional Thin-Layer Chromatography of 17α-Ethinylestradiol on RP-18 W Plate, Detected by Effect-Directed Analysis Using the YES Test

  • We present a two-dimensional (2D) planar chromatographic separation of estrogenic active compounds on RP-18 W (Merck, 1.14296) phase. A mixture of 8 substances was separated using a solvent mix consisting of hexane, ethyl acetate, acetone (55:15:10, v/v) in the first direction and of acetone and water (15:10, v/v) in the second direction. Separation was performed on an RP-18 W plate over aWe present a two-dimensional (2D) planar chromatographic separation of estrogenic active compounds on RP-18 W (Merck, 1.14296) phase. A mixture of 8 substances was separated using a solvent mix consisting of hexane, ethyl acetate, acetone (55:15:10, v/v) in the first direction and of acetone and water (15:10, v/v) in the second direction. Separation was performed on an RP-18 W plate over a distance of 70 mm. This 2D-separation method can be used to quantify 17α-ethinylestradiol (EE2) in an effect-directed analysis, using the yeast strain Saccharomyces cerevisiae BJ3505. The test strain (according to McDonnell) contains the estrogen receptor. Its activation by estrogen active compounds is measured by inducing the reporter gene lacZ which encodes the enzyme β-galactosidase. This enzyme activity is determined on plate by using the fluorescent substrate MUG (4-methylumbelliferyl-β-d-galactopyranoside).show moreshow less

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Metadaten
Author:Yvonne Anna Witos, Barbara Milz, Bernd SpangenbergGND
Publisher:Akadémiai Kiadó
Date of Publication (online):2017/03/16
Language:English
Parent Title (English):JPC - Journal of Planar Chromatography - Modern TLC
Volume:30
Issue:2
ISSN:0933-4173 (Print)
ISSN:1789-0993 (Online)
First Page:136
Last Page:141
Document Type:Article (reviewed)
Institutes:Bibliografie
Acces Right:Zugriffsbeschränkt
Release Date:2018/01/05
Licence (German):License LogoEs gilt das UrhG
DOI:https://doi.org/10.1556/1006.2017.30.2.8