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Grafted 2D-Thin Layer Chromatography (2D-TLC) of 17α-Ethinylestradiol detected by Bio-directed Analysis

  • We present a two dimensional (2D) planar chromatographic separation of estrogenic active compounds on RP-18 (Merck, 1.05559) and silica gel (Merck, 1.05721) phase. A mixture of 13 substances was separated using a solvent mix consisting of methanol–acetonitrile–water (2 + 2 + 1, v/v/v) on RP-18 phase in the first direction and cyclohexane–butylacetate–methanol (8 + 6 + 1, v/v/v) in the secondWe present a two dimensional (2D) planar chromatographic separation of estrogenic active compounds on RP-18 (Merck, 1.05559) and silica gel (Merck, 1.05721) phase. A mixture of 13 substances was separated using a solvent mix consisting of methanol–acetonitrile–water (2 + 2 + 1, v/v/v) on RP-18 phase in the first direction and cyclohexane–butylacetate–methanol (8 + 6 + 1, v/v/v) in the second direction on silica gel plate. Both developments were carried out over a distance of 70 mm. We used the grafted method to combine both plates in a 2D-separation. This 2D-separation method can be used to quantify 17α-ethinylestradiol (EE2) in an effect-directed analysis using the yeast strain Saccharomyces cerevisiae BJ3505. The test strain (according to McDonnell) contains the estrogen receptor. Its activation by estrogen active compounds is measured by inducting the reporter gene lacZ that encodes the enzyme ß-galactosidase. This enzyme activity is determined on plate by using the fluorescent substrate MUG (4-methylumbelliferyl ß-D-galactopyranoside).show moreshow less

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Metadaten
Author:Barbara Milz, Bernd SpangenbergGND
Publisher:Taylor & Francis
Year of Publication:2014
Language:English
GND Keyword:Analyse; Dünnschichtchromatographie; Ethinylestradiol; Polymerisation
Parent Title (English):Journal of Liquid Chromatography & Related Technologies
Volume:37
Issue:20
ISSN:1082-6076
First Page:2872
Last Page:2881
Document Type:Article (reviewed)
Institutes:Hochschule Offenburg / Bibliografie
Acces Right:Zugriffsbeschränkt
Release Date:2017/09/01
Licence (German):License LogoEs gilt das UrhG
DOI:https://doi.org/10.1080/10826076.2014.907109