A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner
- We present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamberWe present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The RF value of triclosan is hRF = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/5219 | Bibliografische Angaben |
Title (English): | A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Barbara AndersStaff MemberGND, Sabrina Doll |
Year of Publication: | 2021 |
Publisher: | Springer International Publishing ; Akadémiai Kiadó |
First Page: | 203 |
Last Page: | 209 |
Parent Title (English): | JPC : Journal of Planar Chromatography - Modern TLC |
Issue: | 34 |
ISSN: | 1789-0993 (Online) |
ISSN: | 0933-4173 (Print) |
DOI: | https://doi.org/10.1007/s00764-021-00108-6 |
URN: | https://urn:nbn:de:bsz:ofb1-opus4-52193 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie | Formale Angaben |
Open Access: | Open Access |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |