Quantification of Diclofenac and Ibuprofen by a Vibrio Fischeri Bioluminescence Assay
- We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95+5+40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using vibrio fischeri bacteria. Within 10 minutes a CCD-cameraWe present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95+5+40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using vibrio fischeri bacteria. Within 10 minutes a CCD-camera registers the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppress the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels [from Santa Barbara Instrument Group, Inc., Santa Barbara, USA]. The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation [1]. The separation method is inexpensive, fast and reliable. Ibuprofen is named after its chemical description: iso-butyl-propanoic phenolic acid. Both pain killers are world-widein use and both substances are stable in aqueous solution. Both substances are mainly excreted in the urine.…
Document Type: | Contribution to a Periodical |
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Zitierlink: | https://opus.hs-offenburg.de/128 | Bibliografische Angaben |
Title (English): | Quantification of Diclofenac and Ibuprofen by a Vibrio Fischeri Bioluminescence Assay |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Andrea SeigelStaff Member, Alexandra Schröck, Rebekka Hauser |
Date of Publication (online): | 2012/07/11 |
First Page: | 55 |
Last Page: | 56 |
Parent Title (German): | Beiträge aus Forschung & Technik |
Volume: | 2011 |
ISSN: | 1866-9352 |
URN: | https://urn:nbn:de:bsz:ofb1-opus-1445 |
Language: | English | Inhaltliche Informationen |
Institutes: | Zentrale Einrichtungen |
Institutes: | Bibliografie |
Journals: | Beiträge aus Forschung & Technik |
DDC classes: | 500 Naturwissenschaften und Mathematik / 540 Chemie |
GND Keyword: | Diclofenac; Environment; Ibuprofen |
Tag: | CCD-array; Vibrio Fischeri Bioluminescence Assay; human health; wastewater | Formale Angaben |
Open Access: | Open Access |
Licence (German): | ![]() |