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Two-Dimensional Thin-Layer Chromatography of 17α-Ethinylestradiol on RP-18 W Plate, Detected by Effect-Directed Analysis Using the YES Test

  • We present a two-dimensional (2D) planar chromatographic separation of estrogenic active compounds on RP-18 W (Merck, 1.14296) phase. A mixture of 8 substances was separated using a solvent mix consisting of hexane, ethyl acetate, acetone (55:15:10, v/v) in the first direction and of acetone and water (15:10, v/v) in the second direction. Separation was performed on an RP-18 W plate over aWe present a two-dimensional (2D) planar chromatographic separation of estrogenic active compounds on RP-18 W (Merck, 1.14296) phase. A mixture of 8 substances was separated using a solvent mix consisting of hexane, ethyl acetate, acetone (55:15:10, v/v) in the first direction and of acetone and water (15:10, v/v) in the second direction. Separation was performed on an RP-18 W plate over a distance of 70 mm. This 2D-separation method can be used to quantify 17α-ethinylestradiol (EE2) in an effect-directed analysis, using the yeast strain Saccharomyces cerevisiae BJ3505. The test strain (according to McDonnell) contains the estrogen receptor. Its activation by estrogen active compounds is measured by inducing the reporter gene lacZ which encodes the enzyme β-galactosidase. This enzyme activity is determined on plate by using the fluorescent substrate MUG (4-methylumbelliferyl-β-d-galactopyranoside).show moreshow less

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Metadaten
Document Type:Article (reviewed)
Zitierlink: https://opus.hs-offenburg.de/2502
Bibliografische Angaben
Title (English):Two-Dimensional Thin-Layer Chromatography of 17α-Ethinylestradiol on RP-18 W Plate, Detected by Effect-Directed Analysis Using the YES Test
Author:Yvonne Anna Witos, Barbara MilzStaff MemberGND, Bernd SpangenbergStaff MemberORCiDGND
Date of Publication (online):2017/03/16
Publisher:Akadémiai Kiadó
First Page:136
Last Page:141
Parent Title (English):JPC - Journal of Planar Chromatography - Modern TLC
Volume:30
Issue:2
ISSN:0933-4173 (Print)
ISSN:1789-0993 (Online)
DOI:https://doi.org/10.1556/1006.2017.30.2.8
Language:English
Inhaltliche Informationen
Institutes:Fakultät Maschinenbau und Verfahrenstechnik (M+V)
Institutes:Bibliografie
Formale Angaben
Open Access: Closed Access 
Licence (German):License LogoUrheberrechtlich geschützt