Quantification of triazine herbicides using chloroplasts in conjunction with thin-layer chromatography
- We present a video-densitometric quantification method for the triazine herbicides atraton, terbumeton, simazine, atrazine and terbutylazine. Triazine herbicides were separated on silica gel using methyl-t-butyl ether, cyclohexane (1+1, v/v) as mobile phase. The quantification was based on a bio-effective-linked analysis using chloroplast and 2,6-dichlorophenolindophenol. Within 1-2 minutesWe present a video-densitometric quantification method for the triazine herbicides atraton, terbumeton, simazine, atrazine and terbutylazine. Triazine herbicides were separated on silica gel using methyl-t-butyl ether, cyclohexane (1+1, v/v) as mobile phase. The quantification was based on a bio-effective-linked analysis using chloroplast and 2,6-dichlorophenolindophenol. Within 1-2 minutes HILL-reaction inhibitor substances show blue-grey zones on a pale yellow-green background. To increase the contrast, the moist plate can be dipped into a solution of PEG-600 (10% PEG-600 in methanol) for 2s. Measurements were carried out using a 16 bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). A white LED was used for illumination purposes. The range of linearity covers more than one magnitude using the (1/R) - 1 expression data transformation. The method can be used for herbicide screenings in environmental samples, because not spectral sensitivity but herbicide activity is measured. The separation method is cheap, fast and reliable.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/9110 | Bibliografische Angaben |
Title (English): | Quantification of triazine herbicides using chloroplasts in conjunction with thin-layer chromatography |
Author: | Melanie BroszatStaff MemberGND, Helena Ernst, Bernd SpangenbergStaff MemberORCiDGND |
Year of Publication: | 2011 |
First Page: | 47 |
Last Page: | 52 |
Parent Title (English): | Environmental Biology |
Volume: | 7 |
Issue: | 2 |
ISSN: | 2391-6095 |
URL: | https://www.environmentalbiotechnology.pl/eb_dzialy/eb_online/2011/vol7_2/ms143bspangenberg.pdf |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Collections of the Offenburg University: | Bibliografie |
Tag: | HPTLC; TLC; bioeffective-linked analysis; derivatization; linear calibration range; quantification; triazine herbicides; video densitometry | Formale Angaben |
Open Access: | Open Access |
Bronze | |
Licence (German): | Urheberrechtlich geschützt |