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The identification and quantification of compounds in the gas phase becomes of increasing interest in the context of environmental protection, as well as in the analytical field. In this respect, the high extinction coefficients of vapours and gases in the ultraviolet wavelength region allow a very sensitive measurement system. In addition, the increased performance of the components necessary for setting up a measurement system, such as fibres, light sources and detectors has been improved. In particular the light sources and detectors offer improved stability, and the deep UV performance and solarisation resistance of fused silica fibres allow have been significantly optimized in the past years. Therefore a compact and reliable detection system with high measuring accuracy is developed. Within this paper possible applications of the system under development and recent results will be discussed.
Das Ziel dieses Projekts ist die Entwicklung und Charakterisierung einer mikrobiellen Brennstoffzelle (MBZ). Die MBZ unterscheidet sich von einer herkömmlichen Brennstoffzelle darin, dass die an der Anode erzeugten Elektronen nicht vom molekularen Wasserstoff, sondern direkt von der im Anodenkompartiment wachsenden Biomasse aus organischen Verbindungen stammen. Die Funktionsweise einer solchen Zelle ist in Abbildung 3.4-1 dargestellt. Im Gegensatz zur herkömmlichen Brennstoffzelle können in einer MBZ auch Abwasserteilströme z. B. aus der Lebensmittelindustrie als Substrat eingesetzt werden. Der große Vorteil der MBZ besteht somit darin, dass Abwässer biologisch abgebaut und gleichzeitig elektrischer Strom erzeugt werden kann.
In contrast to a conventional fuel cell the electrons in a microbial fuel cell (MFC) originate from the metabolic conversion of organic substrates by special bacteria instead of using molecular hydrogen. Recent research in our group has shown that the maximum electrical power density in a MFC correlates with the biomass concentration in batch MFC experiments. In continuous MFC systems additionally the dilution rate D could have an effect on the specific power density. Therefore two steady state conditions are adjusted and the resulting specific power densities, and the biomass and substrate concentrations were measured. These results were implemented in a mathematical description of the continuous MFC-process and the visualization of the model is presented.
Die biologische Verwertung von cellulose-/ hemicellulose- und lignocellulosereichen organischen Substraten zur Erzeugung von Energieträgern gewinnt zunehmend an Bedeutung. Im Gegensatz zu Biokraftstoffen der ersten Generation, bei denen nur ein kleiner Teil des pflanzlichen Materials eingesetzt worden ist (Öl, Zucker, Stärke), wird bei Biokraftstoffen der zweiten Generation fast die vollständige Pflanze einschließlich der schwer zugänglichen Cellulose verwendet. In Biogasanlagen führt diese Zielstellung jedoch häufig zu Problemen. Lignocellulose-reiches Material ist für viele Mikroorganismen schwer oder gar nicht abbaubar. Um die schwer abbaubaren Pflanzenteile wie Cellulose, Hemicellulose oder Lignin den Mikroorganismen in einer Biogasanlage besser zugänglich zu machen, können Biogassubstrate vorbehandelt werden.
The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fedbatch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source. The biomass value reached 3.0 g/l of CDW with 20% of PHAs content within 48 h of cultivation. The polymer was purified from lyophilized cells and analyzed by gas chromatography (GC). The results revealed that the monomeric composition of the obtained polyesters depended on the available substrate. When glucose was used in the growth phase, 3-hydroxyundecanoate and 3- hydroxydodecanoate were found in the polymer composition, whereas in the PHAs-accumulating stage, the Pseudomonas Gl01 strain synthesized mcl-PHAs consisting mainly of 3- hydroxyoctanoate and 3-hydroxydecanoate. The transcriptional analysis using reverse-transcription real-time PCR reaction revealed that the phaC1 gene could be transcribed simultaneously to the phaZ gene.