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A novel peptidyl-lys metalloendopeptidase (Tc-LysN) from Tramates coccinea was recombinantly expressed in Komagataella phaffii using the native pro-protein sequence. The peptidase was secreted into the culture broth as zymogen (~38 kDa) and mature enzyme (~19.8 kDa) simultaneously. The mature Tc-LysN was purified to homogeneity with a single step anion-exchange chromatography at pH 7.2. N-terminal sequencing using TMTpro Zero and mass spectrometry of the mature Tc-LysN indicated that the pro-peptide was cleaved between the amino acid positions 184 and 185 at the Kex2 cleavage site present in the native pro-protein sequence. The pH optimum of Tc-LysN was determined to be 5.0 while it maintained ≥60% activity between pH values 4.5—7.5 and ≥30% activity between pH values 8.5—10.0, indicating its broad applicability. The temperature maximum of Tc-LysN was determined to be 60 °C. After 18 h of incubation at 80 °C, Tc-LysN still retained ~20% activity. Organic solvents such as methanol and acetonitrile, at concentrations as high as 40% (v/v), were found to enhance Tc-LysN’s activity up to ~100% and ~50%, respectively. Tc-LysN’s thermostability, ability to withstand up to 8 M urea, tolerance to high concentrations of organic solvents, and an acidic pH optimum make it a viable candidate to be employed in proteomics workflows in which alkaline conditions might pose a challenge. The nano-LC-MS/MS analysis revealed bovine serum albumin (BSA)’s sequence coverage of 84% using Tc-LysN which was comparable to the sequence coverage of 90% by trypsin peptides.
Blockchain-IIoT integration into industrial processes promises greater security, transparency, and traceability. However, this advancement faces significant storage and scalability issues with existing blockchain technologies. Each peer in the blockchain network maintains a full copy of the ledger which is updated through consensus. This full replication approach places a burden on the storage space of the peers and would quickly outstrip the storage capacity of resource-constrained IIoT devices. Various solutions utilizing compression, summarization or different storage schemes have been proposed in literature. The use of cloud resources for blockchain storage has been extensively studied in recent years. Nonetheless, block selection remains a substantial challenge associated with cloud resources and blockchain integration. This paper proposes a deep reinforcement learning (DRL) approach as an alternative to solving the block selection problem, which involves identifying the blocks to be transferred to the cloud. We propose a DRL approach to solve our problem by converting the multi-objective optimization of block selection into a Markov decision process (MDP). We design a simulated blockchain environment for training and testing our proposed DRL approach. We utilize two DRL algorithms, Advantage Actor-Critic (A2C), and Proximal Policy Optimization (PPO) to solve the block selection problem and analyze their performance gains. PPO and A2C achieve 47.8% and 42.9% storage reduction on the blockchain peer compared to the full replication approach of conventional blockchain systems. The slowest DRL algorithm, A2C, achieves a run-time 7.2 times shorter than the benchmark evolutionary algorithms used in earlier works, which validates the gains introduced by the DRL algorithms. The simulation results further show that our DRL algorithms provide an adaptive and dynamic solution to the time-sensitive blockchain-IIoT environment.
An Overview of Technologies for Improving Storage Efficiency in Blockchain-Based IIoT Applications
(2022)
Since the inception of blockchain-based cryptocurrencies, researchers have been fascinated with the idea of integrating blockchain technology into other fields, such as health and manufacturing. Despite the benefits of blockchain, which include immutability, transparency, and traceability, certain issues that limit its integration with IIoT still linger. One of these prominent problems is the storage inefficiency of the blockchain. Due to the append-only nature of the blockchain, the growth of the blockchain ledger inevitably leads to high storage requirements for blockchain peers. This poses a challenge for its integration with the IIoT, where high volumes of data are generated at a relatively faster rate than in applications such as financial systems. Therefore, there is a need for blockchain architectures that deal effectively with the rapid growth of the blockchain ledger. This paper discusses the problem of storage inefficiency in existing blockchain systems, how this affects their scalability, and the challenges that this poses to their integration with IIoT. This paper explores existing solutions for improving the storage efficiency of blockchain–IIoT systems, classifying these proposed solutions according to their approaches and providing insight into their effectiveness through a detailed comparative analysis and examination of their long-term sustainability. Potential directions for future research on the enhancement of storage efficiency in blockchain–IIoT systems are also discussed.
Linux and Linux-based operating systems have been gaining more popularity among the general users and among developers. Many big enterprises and large companies are using Linux for servers that host their websites, some even require their developers to have knowledge about Linux OS. Even in embedded systems one can find many Linux-based OS that run them. With its increasing popularity, one can deduce the need to secure such a system that many personnel rely on, be it to protect the data that it stores or to protect the integrity of the system itself, or even to protect the availability of the services it offers. Many researchers and Linux enthusiasts have been coming up with various ways to secure Linux OS, however new vulnerabilities and new bugs are always found, by malicious attackers, with every update or change, which calls for the need of more ways to secure these systems.
This Thesis explores the possibility and feasibility of another way to secure Linux OS, specifically securing the terminal of such OS, by altering the commands of the terminal, getting in the way of attackers that have gained terminal access and delaying, giving more time for the response teams and for forensics to stop the attack, minimize the damage, restore operations, and to identify collect and store evidence of the cyber-attack. This research will discuss the advantages and disadvantages of various security measures and compare and contrast with the method suggested in this research.
This research is significant because it paints a better picture of what the state of the art of Linux and Linux-based operating systems security looks like, and it addresses the concerns of security enthusiasts, while exploring new uncharted area of security that have been looked at as a not so significant part of protecting the OSes out of concern of the various limitations and problems it entails. This research will address these concerns while exploring few ways to solve them, as well as addressing the ideal areas and situations in which the proposed method can be used, and when would such method be more of a burden than help if used.
An der Offenburger Hochschule wurde eine neue Art der Ansteuerungsmethode für Handprothesen und -orthesen entwickelt, die auf der Verwendung einer Augmented Reality Brille basiert. Dieses neue Prothesensystem soll in einer ersten Studie an Probanden auf seine Alltagstauglichkeit getestet werden. Ziel dieser Arbeit ist es, die regulatorischen Anforderungen an eine solche Studie zusammenzustellen, mit Schwerpunkt auf einem Antrag bei einer Ethikkommission. Außerdem sind mittels Literaturrecherche Tests zu identifizieren und zu analysieren, die für die Beurteilung von Handprothesen verwendet werden. Hierfür wird erörtert was Alltagstauglichkeit bedeutet und welche Eigenschaften und Ziele identifizierte Tests haben.
Germany was considered the world's export champion for a long time, until it was overtaken by China in 2009. Both nations provide officially supported export credits to national exporting organizations, but the two systems operate differently. German export credit guarantees serve as a substitute when the private market is unable to assume the risks of exporting companies. The German Export Credit Agency Euler Hermes is responsible for processing applications on behalf of the Federal Government. China belongs to the largest providers of export finance with the institutions China EXIM and Sinosure. While Germany is bound by the OECD consensus, which defines the level playing field, Chinese export credit agencies have greater flexibility not being bound by international rules or agreements.
Die Wertschöpfung vorherrschender Datenmengen scheitert, obgleich diese als der Treibstoff der Zukunft gelten, oftmals an den grundlegendsten Dingen. Das Digitalisierungs- und auch Verlagerungsverhalten werden für das Content Management (CM) zunehmend zu einem herausfordernden Fallstrick.
Die Unternehmen sind mit Fragestellungen traktiert, die sich darauf referenzieren, EchtzeitStröme unstrukturierter Daten aus heterogenen Quellen zu analysieren und zu speichern.
Trotz aller Bemühungen, die unaufhaltsam wachsende Menge an Daten- beziehungsweise Content im Rahmen eines effizienten Managements künftig manuell in den Griff zu bekommen, scheint es, als ob die Unternehmen an der kaum zu bewerkstelligenden Herausforderung scheitern werden.
Die vorliegende Arbeit untersucht, inwieweit es einer innovativen Technologie, wie der Künstlichen Intelligenz (KI) gelingen kann, das Content Management nachhaltig zu revolutionieren und damit den Content in seinem Umfang so zu organisieren und zu nutzen, um den Unternehmen eine Perspektive zu bieten, die steigende Welle an Big Data zu bewältigen.
Somit bewegt sich diese Arbeit auf dem Forschungsfeld der KI, als Teilgebiet der Informatik, die enorme Chancen und gleichzeitig Herausforderungen für die Wissenschaft und die Innovationsfähigkeit der Unternehmen mit sich bringt.
Im Rahmen qualitativer Expert*inneninterviews als Lösungsansatz wurde untersucht, inwiefern es KI-gestützten Systemen gelingen kann, Wissensmitarbeiter*innen entlang des Content Life Cycles zu unterstützen und den Nutzer*innen bezüglich der Ausspielung der Inhalte eine optimale Customer Experience zu bieten.
Die fehlende Nachvollziehbarkeit und das Missverständnis des KI-Begriffes sowie die Kluft zwischen der öffentlichen Debatte und der Realität der KI erweisen sich hierbei als die wohl größten Innovationsbremsen des KI-Einsatzes in der Content Management Umgebung.
Die Ergebnisse der Arbeit tragen im Wesentlichen dazu bei, das Verständnis für die KI zu schärfen und gleichzeitig das aufkommende Dilemma des Vertrauensdefizites der Mensch-Maschine-Kommunikation zu entschärfen.
Außerdem wird ein Grundverständnis dafür geschaffen, die KI als geeignetes Tool im Content Management zu erkennen.
Darüber hinaus wird demonstriert, dass sich durch den Einsatz der KI im Content Management ebenfalls immense Vorteile für die Ausspielung user*innenspezifierten Contents ergeben, die im folgenden Verlauf genauer aufgeführt werden.
AI-based Ground Penetrating Radar Signal Processing for Thickness Estimation of Subsurface Layers
(2023)
This thesis focuses on the estimation of subsurface layer thickness using Ground Penetrating Radar (GPR) A-scan and B-scan data through the application of neural networks. The objective is to develop accurate models capable of estimating the thickness of up to two subsurface layers.
Two different approaches are explored for processing the A-scan data. In the first approach, A-scans are compressed using Principal Component Analysis (PCA), and a regression feedforward neural network is employed to estimate the layers’ thicknesses. The second approach utilizes a regression one-dimensional Convolutional Neural Network (1-D CNN) for the same purpose. Comparative analysis reveals that the second approach yields superior results in terms of accuracy.
Subsequently, the proposed 1-D CNN architecture is adapted and evaluated for Step Frequency Continuous Wave (SFCW) radar, expanding its applicability to this type of radar system. The effectiveness of the proposed network in estimating subsurface layer thickness for SFCW radar is demonstrated.
Furthermore, the thesis investigates the utilization of GPR B-scan images as input data for subsurface layer thickness estimation. A regression CNN is employed for this purpose, although the results achieved are not as promising as those obtained with the 1-D CNN using A-scan data. This disparity is attributed to the limited availability of B-scan data, as B-scan generation is a resource-intensive process.
Deutsche Banken begleiten vielfältige Geschäfte mit Auslandsbezug. Vor allem Kreditgeschäfte und Akkreditive sind die häufigsten Geschäftsarten, an denen deutsche Banken als Finanzierungspartei gemeinschaftlich mit anderen ausländischen Finanzinstituten auftreten. Im Rahmen solcher Geschäfte verlangen ausländische Geschäftspartner häufig die Einhaltung von ausländischen Sanktionsvorschriften und verankern dies in den vertraglichen Dokumenten. Beteiligen sich Finanzinstitute, beispielsweise als Kreditnehmer, so wird die Einhaltung der ausländischen Sanktionsvorschriften direkt von den Finanzinstituten verlangt. Treten jedoch Finanzinstitute als Kreditgeber auf, was eher häufiger der Fall ist, so fordern die Finanzinstitute den Kreditnehmer auf, ausländisches Sanktionsrecht einzuhalten. Die Verpflichtung zur Einhaltung von ausländischen Sanktionsvorschriften widerspricht den Anti-Boykott-Regelungen auf nationaler und gegebenenfalls auf europäischer Ebene.