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BACKGROUND
Various neutral and alkaline peptidases are commercially available for use in protein hydrolysis under neutral to alkaline conditions. However, the hydrolysis of proteins under acidic conditions by applying fungal aspartic peptidases (FAPs) has not been investigated in depth so far. The aim of this study, thus, was to purify a FAP from the commercial enzyme preparation, ROHALASE® BXL, determine its biochemical characteristics, and investigate its application for the hydrolysis of food and animal feed proteins under acidic conditions.
RESULTS
A Trichoderma reesei derived FAP, with an apparent molecular mass of 45.8 kDa (sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SDS-PAGE) was purified 13.8-fold with a yield of 37% from ROHALASE® BXL. The FAP was identified as an aspartate protease (UniProt ID: G0R8T0) by inhibition and nano-LC-ESI-MS/MS studies. The FAP showed the highest activity at 50°C and pH 4.0. Monovalent cations, organic solvents, and reducing agents were tolerated well by the FAP. The FAP underwent an apparent competitive product inhibition by soy protein hydrolysate and whey protein hydrolysate with apparent Ki-values of 1.75 and 30.2 mg*mL−1, respectively. The FAP showed promising results in food (soy protein isolate and whey protein isolate) and animal feed protein hydrolyses. For the latter, an increase in the soluble protein content of 109% was noted after 30 min.
CONCLUSION
Our results demonstrate the applicability of fungal aspartic endopeptidases in the food and animal feed industry. Efficient protein hydrolysis of industrially relevant substrates such as acidic whey or animal feed proteins could be conducted by applying fungal aspartic peptidases. © 2022 Society of Chemical Industry.
Significant improvements in module performance are possible via implementation of multi-wire electrodes. This is economically sound as long as the mechanical yield of the production is maintained. While flat ribbons have a relatively large contact area to exert forces onto the solar cell, wires with round cross section reduce this contact area considerably – in theory to an infinitively thin line. Therefore, the local stresses induced by the electrodes might increase to a point that mechanical production yields suffer unacceptably.
In this paper, we assess this issue by an analytical mechanical model as well as experiments with an encapsulant-free N.I.C.E. test setup. From these, we can derive estimations for the relationship between lay-up accuracy and expected breakage losses. This paves the way for cost-optimized choices of handling equipment in industrial N.I.C.E.-wire production lines.
Micronization of biochar (BC) may ease its application in agriculture. For example, fine biochar powders can be applied as suspensions via drip-irrigation systems or can be used to produce grnulated fertilizers. However, micronization may effect important physical biochar properties like the water holding capacity (WHC) or the porosity.
Weitsprung mit und ohne Unterschenkelprothese – gleiche Sportart, unterschiedliche Disziplinen
(2022)
The majority of anterior cruciate ligament (ACL) injuries in team sports are non-contact injuries, with cutting maneuvers identified as high-risk tasks. Young female handball players have been shown to be at greater risk for ACL injuries than males. One risk factor for ACL injuries is the magnitude of the knee abduction moment (KAM). Cutting technique variables on foot placement, overall approach and knee kinematics have been shown to influence the KAM. Since injury risk is believed to increase with increasing task complexity, the purpose of the study was to test the effect of task complexity on technique variables that influence the KAM in female handball players during fake-and-cut tasks.
In dem Projekt BioMeth wurde der Ansatz der Membranbegasung zur Erhöhung der Verfügbarkeit von gelöstem Wasserstoff für die biologische Methanisierung im Sinn der Etablierung eines Power-to-Gas-Konzeptes zur Energiespeicherung verfolgt. Übergeordnetes Ziel war die Entwicklung eines skalierbaren Verfahrenskonzeptes, dass sich zur Nutzung CO2-haltiger Gasvolumenström eignet. Geplant war es, das Verfahren am Beispiel der Biogasanlage der Biokäserei Monte-Ziego in Teningen zu demonstrieren und dort das bestehende Konzept der parallelen Abwasseraufbereitung und Energieerzeugung zu erweitern. Die ursprüngliche Struktur des Arbeitspaketplanes ist in nachfolgender Abbildung gezeigt.
Konstrukteure im Maschinenbau stehen häufig vor der Problemstellung, hochfest vorgespannte Schraubenverbindungen und einen durchgehenden Korrosionsschutz zu vereinen. Die einschlägigen Normen und Richtlinien bieten hierzu Stand heute keine ausreichende Hilfestellung. In diesem Beitrag werden an Versuchsblechen ermittelte Setzbeträge von maschinenbautypischen organischen Beschichtungssystemen unter Variation der Belastungshöhe und der Umgebungstemperatur präsentiert und mit in Bauteilversuchen gemessenen Vorspannkraftverlusten vergleichend bewertet.
TRIZ Inventive Principles
(2022)
The analysis of several thousand patents led to the conclusion that inventive engineering problems and technical contradictions in all kinds of industrial sectors could be solved by a limited number of basic Inventive Principles (Altshuller, 1984). The modern Theory of Inventive Problem Solving TRIZ (VDI 4521) contains 40 basic Inventive Principles (IP). These principles are simple to use or modify and can be easily integrated in brainstorming or daily engineer’s work. One established part of industrial practice is the composition of the specific groups of principles for solving different kinds of problems (Livotov, Petrov, 2011). Based on interdisciplinary experience of TRIZ application in the industrial companies in the last 25 years the a general order in the application of 40 Inventive Principles can be recommended for idea generation and problem solving (Livotov, Chandra, Mas'udah et al, 2019). This brochure presents an update of the 40 Inventive Principles extending the original version (Altshuller, 1984) with additional 70 sub-principles, resulting in the advanced set of 160 sub-principles, regarded as elementary inventive operators. These extended version of inventive principles finds its application in the AIDA Automatic IDEA & IP Generator https://www.tris-europe.com/eng/software/innovationssoftware.htm
With recent developments in the Ukrainian-Russian conflict, many are discussing about Germany’s dependency on fossil fuel imports in its energy system, and how can the country proceed with reducing that dependency. With its wide-ranging consumption sectors, the electricity sector comes as the perfect choice to start with. Recent reports showed that the German federal government is already intending to have a fully renewable electricity by 2035 while exploiting all possible clean power options. This was published in the federal government’s climate emergency program (Easter Package) in early 2022. The aim of this package is to initiate a rapid transition and decarbonization of the electricity sector. The Easter Package expects an enormous growth of renewable energies to a completely new level, with already at least 80% renewable gross energy consumption, with extensive and broad deployment of different generation technologies on various scales. This paper will discuss this ambitious plan and outline some insights into this huge and rapidly increasing step, and show how much will Germany need in order to achieve this huge milestone towards a fully green supply of the electricity sector. Different scenarios and shares of renewables will be investigated in order to elaborate on preponed climate-neutral goal of the electricity sector by 2035. The results pointed out some promising aspects in achieving a 100% renewable power, with massive investments in both generation and storage technologies.
Running shoes were categorized either as motion control, cushioned, or minimal footwear in the past. Today, these categories blur and are not as clearly defined. Moreover, with the advances in manufacturing processes, it is possible to create individualized running shoes that incorporate features that meet individual biomechanical and experiential needs. However, specific ways to individualize footwear to reduce individual injury risk are poorly understood. Therefore, the purpose of this scoping review was to provide an overview of (1) footwear design features that have the potential for individualization; (2) human biomechanical variability as a theoretical foundation for individualization; (3) the literature on the differential responses to footwear design features between selected groups of individuals. These purposes focus exclusively on reducing running-related risk factors for overuse injuries. We included studies in the English language on adults that analyzed: (1) potential interaction effects between footwear design features and subgroups of runners or covariates (e.g., age, gender) for running-related biomechanical risk factors or injury incidences; (2) footwear perception for a systematically modified footwear design feature. Most of the included articles (n = 107) analyzed male runners. Several footwear design features (e.g., midsole characteristics, upper, outsole profile) show potential for individualization. However, the overall body of literature addressing individualized footwear solutions and the potential to reduce biomechanical risk factors is limited. Future studies should leverage more extensive data collections considering relevant covariates and subgroups while systematically modifying isolated footwear design features to inform footwear individualization.
Modelbasierte Zustandsschätzung elektrischer Betriebsmittel der Mittel- und Niederspannungsebenen
(2022)
Im Projekt MOBCOM wird ein neues Verfahren zur Zustandsüberwachung von elektrischen Betriebsmitteln in Niederspannungsnetzen und Anlagen entwickelt. Mittels PLC (power line communication) Technologie werden hochfrequente transiente Vorgänge auf dem Stromkanal und dessen Übertragungseigenschaften erfasst und bewertet.
In dem Abschlussbericht wird ein Prototyp für Powerline-Kommunikation zur Netzüberwachung beschrieben. Der Prototyp basiert auf einem PLC-Empfänger, welcher den Kanal misst, um so Informationen über den PLC-Kanal und den aktuellen Zustand des Stromnetzes zu erhaltet. Der PLC-Empfänger verwendet das Kommunikationssignal, um eine genaue Schätzung des Stromkanals zu erhalten und liefert Informationen zur Erkennung von Teilentladungen und anderen Anomalien im Netz. Diese Überwachung des Stromnetzes macht sich die bestehende PLC-Infrastruktur zunutze und verwendet die ohnehin übertragenen Datensignale, um eine Echtzeitmessung der Kanalübertragungsfunktion und des empfangenen Rauschsignals zu erhalten. Da dieses Signal im Vergleich zu einfacheren Messsensoren mit einer hohen Abtastrate abgetastet wird, enthält es wertvolle Informationen über mögliche Beeinträchtigungen im Netz, die behoben werden müssen. Während die Kanalmessungen auf einem empfangenen PLC-Signal beruhen, können Informationen über Teilentladungen oder andere Störquellen allein durch einen PLC-Empfänger gesammelt werden, d. h. ohne eine PLC-Übertragung. Es wurde ein Prototyp auf Basis von Software Defined Radio entwickelt, der die gleichzeitige Kommunikation und Erfassung für ein Stromnetz implementiert.