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In 4D printing, an additively manufactured component is given the ability to change its shape or function in an intended and useful manner over time. The technology of 4D printing is still in an early stage of development. Nevertheless, interesting research and initial applications exist in the literature. In this work, a novel methodical approach is presented that helps transfer existing 4D printing research results and knowledge into solving application tasks systematically. Moreover, two different smart materials are analyzed, used, and combined following the presented methodical approach to solving the given task in the form of recovering an object from a poorly accessible space. This is implemented by self-positioning, grabbing, and extracting the target object. The first smart material used to realize these tasks is a shape-memory polymer, while the second is a polymer-based magnetic composite. In addition to the presentation and detailed implementation of the methodical approach, the potentials and behavior of the two smart materials are further examined and narrowed down as a result of the investigation. The results show that the developed methodical approach contributes to moving 4D printing closer toward a viable alternative to existing technologies due to its problem-oriented nature.
A novel peptidyl-lys metalloendopeptidase (Tc-LysN) from Tramates coccinea was recombinantly expressed in Komagataella phaffii using the native pro-protein sequence. The peptidase was secreted into the culture broth as zymogen (~38 kDa) and mature enzyme (~19.8 kDa) simultaneously. The mature Tc-LysN was purified to homogeneity with a single step anion-exchange chromatography at pH 7.2. N-terminal sequencing using TMTpro Zero and mass spectrometry of the mature Tc-LysN indicated that the pro-peptide was cleaved between the amino acid positions 184 and 185 at the Kex2 cleavage site present in the native pro-protein sequence. The pH optimum of Tc-LysN was determined to be 5.0 while it maintained ≥60% activity between pH values 4.5—7.5 and ≥30% activity between pH values 8.5—10.0, indicating its broad applicability. The temperature maximum of Tc-LysN was determined to be 60 °C. After 18 h of incubation at 80 °C, Tc-LysN still retained ~20% activity. Organic solvents such as methanol and acetonitrile, at concentrations as high as 40% (v/v), were found to enhance Tc-LysN’s activity up to ~100% and ~50%, respectively. Tc-LysN’s thermostability, ability to withstand up to 8 M urea, tolerance to high concentrations of organic solvents, and an acidic pH optimum make it a viable candidate to be employed in proteomics workflows in which alkaline conditions might pose a challenge. The nano-LC-MS/MS analysis revealed bovine serum albumin (BSA)’s sequence coverage of 84% using Tc-LysN which was comparable to the sequence coverage of 90% by trypsin peptides.
In this paper, a temperature-dependent viscoplasticity model is presented that describes thermal and cyclic softening of the hot work steel X38CrMoV5-3 under thermomechanical fatigue loading. The model describes the softening state of the material by evolution equations, the material properties of which can be determined on the basis of a defined experimental program. A kinetic model is employed to capture the effect of coarsening carbides and a new isotropic cyclic softening model is developed that takes history effects during thermomechanical loadings into account. The temperature-dependent material properties of the viscoplasticity model are determined on the basis of experimental data measured in isothermal and thermomechanical fatigue tests for the material X38CrMoV5-3 in the temperature range between 20 and 650 ∘C. The comparison of the model and an existing model for isotropic softening shows an improved description of the softening behavior under thermomechanical fatigue loading. A good overall description of the experimental data is possible with the presented viscoplasticity model, so that it is suited for the assessment of operating loads of hot forging tools.
For the treatment of bone defects, biodegradable, compressive biomaterials are needed as replacements that degrade as the bone regenerates. The problem with existing materials has either been their insufficient mechanical strength or the excessive differences in their elastic modulus, leading to stress shielding and eventual failure. In this study, the compressive strength of CPC ceramics (with a layer thickness of more than 12 layers) was compared with sintered β-TCP ceramics. It was assumed that as the number of layers increased, the mechanical strength of 3D-printed scaffolds would increase toward the value of sintered ceramics. In addition, the influence of the needle inner diameter on the mechanical strength was investigated. Circular scaffolds with 20, 25, 30, and 45 layers were 3D printed using a 3D bioplotter, solidified in a water-saturated atmosphere for 3 days, and then tested for compressive strength together with a β-TCP sintered ceramic using a Zwick universal testing machine. The 3D-printed scaffolds had a compressive strength of 41.56 ± 7.12 MPa, which was significantly higher than that of the sintered ceramic (24.16 ± 4.44 MPa). The 3D-printed scaffolds with round geometry reached or exceeded the upper limit of the compressive strength of cancellous bone toward substantia compacta. In addition, CPC scaffolds exhibited more bone-like compressibility than the comparable β-TCP sintered ceramic, demonstrating that the mechanical properties of CPC scaffolds are more similar to bone than sintered β-TCP ceramics.
Most recently, the federal government in Germany published new climate goals in order reach climate neutrality by 2045. This paper demonstrates a path to a cost optimal energy supply system for the German power grid until the year 2050. With special regard to regionality, the system is based on yearly myopic optimization with the required energy system transformation measures and the associated system costs. The results point out, that energy storage systems (ESS) are fundamental for renewables integration in order to have a feasible energy transition. Moreover, the investment in storage technologies increased the usage of the solar and wind technologies. Solar energy investments were highly accompanied with the installation of short-term battery storage. Longer-term storage technologies, such as H2, were accompanied with high installations of wind technologies. The results pointed out that hydrogen investments are expected to overrule short-term batteries if their cost continues to decrease sharply. Moreover, with a strong presence of ESS in the energy system, biomass energy is expected to be completely ruled out from the energy mix. With the current emission reduction strategy and without a strong presence of large scale ESS into the system, it is unlikely that the Paris agreement 2° C target by 2050 will be achieved, let alone the 1.5° C.
In a randomized controlled cross-over study ten male runners (26.7 ± 4.9 years; recent 5-km time: 18:37 ± 1:07 min:s) performed an incremental treadmill test (ITT) and a 3-km time trial (3-km TT) on a treadmill while wearing either carbon fiber insoles with downwards curvature or insoles made of butyl rubber (control condition) in light road racing shoes (Saucony Fastwitch 9). Oxygen uptake, respiratory exchange ratio, heart rate, blood lactate concentration, stride frequency, stride length and time to exhaustion were assessed during ITT. After ITT, all runners rated their perceived exertion, perceived shoe comfort and perceived shoe performance. Running time, heart rate, blood lactate levels, stride frequency and stride length were recorded during, and shoe comfort and shoe performance after, the 3-km TT. All parameters obtained during or after the ITT did not differ between the two conditions [range: p = 0.188 to 0.948 (alpha value: 0.05); Cohen's d = 0.021 to 0.479] despite the rating of shoe comfort showing better scores for the control insoles (p = 0.001; d = −1.646). All parameters during and after the 3-km TT showed no differences (p = 0.200 to 1.000; d = 0.000 to 0.501) between both conditions except for shoe comfort showing better scores for control insoles (p = 0.017; d = −0.919). Running with carbon fiber insoles with downwards curvature did not change running performance or any submaximal or maximal physiological or biomechanical parameter and perceived exertion compared to control condition. Shoe comfort is impaired while running with carbon fiber insoles. Wearing carbon fiber insoles with downwards curvature during treadmill running is not beneficial when compared to running with control insoles.
In the literature, many studies have described the 3D printing of ceramic-based scaffolds (e.g., printing with calcium phosphate cement) in the form of linear structures with layer rotations of 90°, although no right angles can be found in the human body. Therefore, this work focuses on the adaptation of biological shapes, including a layer rotation of only 1°. Sample shapes were printed with calcium phosphate cement using a 3D Bioplotter from EnvisionTec. Both straight and wavy spokes were printed in a round structure with 12 layers. Depending on the strand diameter (200 and 250 µm needle inner diameter) and strand arrangement, maximum failure loads of 444.86 ± 169.39 N for samples without subsequent setting in PBS up to 1280.88 ± 538.66 N after setting in PBS could be achieved.
Objective: This paper deals with the design and the optimization of mechatronic devices.
Introduction: Comparing with existing works, the design approach presented in this paper aims to integrate optimization in the design phase of complex mechatronic systems in order to increase the efficiency of this method.
Methods: To solve this problem, a novel mechatronic system design approach has been developed in order to take the multidisciplinary aspect and to consider optimization as a tool that can be used within the embodiment design process to build mechatronic solutions from a set of solution concepts designed with innovative or routine design methods.
Conclusions: This approach has then been applied to the design and optimization of a wind turbine system that can be implemented to autonomously supply a mountain cottage.
Over the last few decades, several grid coupling techniques for hierarchically refined Cartesian grids have been developed to provide the possibility of varying mesh resolution in lattice Boltzmann methods. The proposed schemes can be roughly categorized based on the individual grid transition interface layout they are adapted to, namely cell-vertex or cell-centered approaches, as well as a combination of both. It stands to reason that the specific properties of each of these grid-coupling algorithms influence the stability and accuracy of the numerical scheme. Consequently, this naturally leads to a curiosity regarding the extent to which this is the case. The present study compares three established grid-coupling techniques regarding their stability ranges by conducting a series of numerical experiments for a square duct flow, including various collision models. Furthermore the hybrid-recursive regularized collision model, originally introduced for cell-vertex algorithms with co-located coarse and fine grid nodes, has been adapted to cell-centered and combined methods.
Photovoltaic-heat pump (PV-HP) combinations with battery and energy management systems are becoming increasingly popular due to their ability to increase the autarchy and utilization of self-generated PV electricity. This trend is driven by the ongoing electrification of the heating sector and the growing disparity between growing electricity costs and reducing feed-in tariffs in Germany. Smart control strategies can be employed to control and optimize the heat pump operation to achieve higher self-consumption of PV electricity. This work presents the evaluation results of a smart-grid ready controlled PV-HP-battery system in a single-family household in Germany, using 1-minute-high-resolution field measurement data. Within 12 months evaluation period, a self-consumption of 43% was determined. The solar fraction of the HP amounts to 36%, enabled also due to higher set temperatures for space heating and domestic hot water production. Accordingly, the SPF decreases by 4.0% the space heating and by 5.7% in the domestic hot water mode. The combined seasonal performance factor for the heat pump system increases from 4.2 to 6.7, when only considering the electricity taken from the grid and disregarding the locally generated electricity supplied from photovoltaic and battery units.
Young female handball players represent a high-risk population for anterior cruciate ligament (ACL) injuries. While the external knee abduction moment (KAM) is known to be a risk factor, it is unclear how cutting technique affects KAMs in sport-specific cutting maneuvers. Further, the effect of added game specificity (e.g., catching a ball or faking defenders) on KAMs and cutting technique remains unknown. Therefore, this study aimed: (i) to test if athletes grouped into different clusters of peak KAMs produced during three sport-specific fake-and-cut tasks of different complexities differ in cutting technique, and (ii) to test whether technique variables change with task complexity. Fifty-one female handball players (67.0 ± 7.7 kg, 1.70 ± 0.06 m, 19.2 ± 3.4 years) were recruited. Athletes performed at least five successful handball-specific sidestep cuts of three different complexities ranging from simple pre-planned fake-and-cut maneuvers to catching a ball and performing an unanticipated fake-and-cut maneuver with dynamic defenders. A k-means cluster algorithm with squared Euclidean distance metric was applied to the KAMs of all three tasks. The optimal cluster number of koptimal = 2 was calculated using the average silhouette width. Statistical differences in technique variables between the two clusters and the tasks were analyzed using repeated-measures ANOVAs (task complexity) with nested groupings (clusters). KAMs differed by 64.5%, on average, between clusters. When pooling all tasks, athletes with high KAMs showed 3.4° more knee valgus, 16.9% higher downward and 8.4% higher resultant velocity at initial ground contact, and 20.5% higher vertical ground reaction forces at peak KAM. Unlike most other variables, knee valgus angle was not affected by task complexity, likely due to it being part of inherent movement strategies and partly determined by anatomy. Since the high KAM cluster showed higher vertical center of mass excursions and knee valgus angles in all tasks, it is likely that this is part of an automated motor program developed over the players' careers. Based on these results, reducing knee valgus and downward velocity bears the potential to mitigate knee joint loading and therefore ACL injury risk.
Building energy management systems (BEMSs), dedicated to sustainable buildings, may have additional duties, such as hosting efficient energy management systems (EMSs) algorithms. This duty can become crucial when operating renewable energy sources (RES) and eventual electric energy storage systems (ESSs). Sophisticated EMS approaches that aim to manage RES and ESSs in real time may need high computing capabilities that BEMSs typically cannot provide. This article addresses and validates a fuzzy logic-based EMS for the optimal management of photovoltaic (PV) systems with lead-acid ESSs using an edge computing technology. The proposed method is tested on a real smart grid prototype in comparison with a classical rule-based EMS for different weather conditions. The goal is to investigate the efficacy of islanding the building local network as a control command, along with ESS power control. The results show the implementation feasibility and performance of the fuzzy algorithm in the optimal management of ESSs in both operation modes: grid-connected and islanded modes.
Biomechanical Risk Factors of Injury-Related Single-Leg Movements in Male Elite Youth Soccer Players
(2022)
Altered movement patterns during single-leg movements in soccer increase the risk of lower-extremity non-contact injuries. The identification of biomechanical parameters associated with lower-extremity injuries can enrich knowledge of injury risks and facilitate injury prevention. Fifty-six elite youth soccer players performed a single-leg drop landing task and an unanticipated side-step cutting task. Three-dimensional ankle, knee and hip kinematic and kinetic data were obtained, and non-contact lower-extremity injuries were documented throughout the season. Risk profiling was assessed using a multivariate approach utilising a decision tree model (classification and regression tree method). The decision tree model indicated peak knee frontal plane angle, peak vertical ground reaction force, ankle frontal plane moment and knee transverse plane angle at initial contact (in this hierarchical order) for the single-leg landing task as important biomechanical parameters to discriminate between injured and non-injured players. Hip sagittal plane angle at initial contact, peak ankle transverse plane angle and hip sagittal plane moment (in this hierarchical order) were indicated as risk factors for the unanticipated cutting task. Ankle, knee and hip kinematics, as well as ankle and hip kinetics, during single-leg high-risk movements can provide a good indication of injury risk in elite youth soccer players.
Recently, photovoltaic (PV) with energy storage systems (ESS) have been widely adopted in buildings to overcome growing power demands and earn financial benefits. The overall energy cost can be optimized by combining a well-sized hybrid PV/ESS system with an efficient energy management system (EMS). Generally, EMS is implemented within the overall functions of the Building Automation System (BAS). However, due to its limited computing resources, BAS cannot handle complex algorithms that aim to optimize energy use in real-time under different operating conditions. Furthermore, islanding the building's local network to maximize the PV energy share represents a challenging task due to the potential technical risks. In this context, this article addresses an improved approach based on upgrading the BAS data analytics capability by means of an edge computing technology. The edge communicates with the BAS low-level controller using a serial communication protocol. Taking advantage of the high computing ability of the edge device, an optimization-based EMS of the PV/ESS hybrid system is implemented. Different testing scenarios have been carried out on a real prototype with different weather conditions, and the results show the implementation feasibility and technical performance of such advanced EMS for the management of building energy resources. It has also been proven to be feasible and advantageous to operate the local energy network in island mode while ensuring system safety. Additionally, an estimated energy saving improvement of 6.23 % has been achieved using optimization-based EMS compared to the classical rule-based EMS, with better ESS constraints fulfillment.
Im Automobilbau bietet der Einsatz der Multimaterialbauweise ein signifikantes Potenzial zur Gewichtsreduktion. Zugleich erfordert diese Bauweise eine große Anzahl von Fügeverfahren für die Verbindung der unterschiedlichen Werkstoffe und Werkstoffklassen. Dabei muss eine Vielzahl an konstruktiven und materialseitigen Anforderungen berücksichtigt werden. Um in diesem Auswahlprozess den Aspekt des Leichtbaus beim Fügeverfahren selbst systematisch zu integrieren, wurde eine Methodik entwickelt, welche die Fügeverfahren im Hinblick auf ihr jeweiliges Leichtbaupotenzial bewertet.
A smart energy concept was designed and implemented for a cluster of 5 existing multi-family houses, which combines heat pumps, photovoltaic (PV) modules and combined heat and power units (CHP) to achieve energy- and cost-efficient operation. Measurement results of the first year of operation show that the local power generation by PV modules and CHP unit has a positive effect on the electrical self-sufficiency by reducing electricity import from the grid. In winter, when the CHP unit operates continuously for long periods, the entire electricity for the heat pump and 91 % of the total electricity demand of the neighborhood are supplied locally. In summer, only 53 % is generated within the neighborhood. The use of a specifically developed energy management system EMS is intended to further increase this share. CO2 emissions for heating and electricity of the neighborhood are evaluated and amount to 18.4 kg/(m2a). Compared to the previous energy system consisting of gas boilers (29.1 kg/(m2a)), savings of 37 % are achieved with electricity consumption from the grid being reduced by 65 %. In the second construction stage, an additional heat pump, CHP unit and PV modules will be added. The measurement results indicate that the final district energy system is likely to achieve the ambitious CO2 reduction goal of -50% and further increase the self-sufficiency of the district.
Enzyme‐assisted HPTLC method for the simultaneous analysis of inositol phosphates and phosphate
(2023)
Background
The analysis of myo‐inositol phosphates (InsPx) released by phytases during phytic acid degradation is challenging and time‐consuming, particularly in terms of sample preparation, isomer separation, and detection. However, a fast and robust analysis method is crucial when screening for phytases during protein engineering approaches, which result in a large number of samples, to ensure reliable identification of promising novel enzymes or target variants with improved characteristics, for example, pH range, thermal stability, and phosphate release kinetics.
Results
The simultaneous analysis of several InsPx (InsP1‐InsP4 and InsP5 + 6) as well as free phosphate was established on cellulose HPTLC plates using a buffered mobile phase. Inositol phosphates were subsequently stained using a novel enzyme‐assisted staining procedure. Immobilized InsPx were hydrolyzed by a phytase solution of Quantum® Blueliquid 5G followed by a molybdate reagent derivatization. Resulting blue zones were captured by DAD scan. The method shows good repeatability (intra‐day and intra‐lab) with maximum deviations of the Rf value of 0.01. The HPTLC method was applied to three commercially available phytases at two pH levels relevant to the gastrointestinal tract of poultry (pH 5.5 and pH 3.6) to observe their phytate degradation pattern and thus visualize their InsPx fingerprint.
Conclusion
This HPTLC method presents a semi‐high‐throughput analysis for the simultaneous analysis of phytic acid and the resulting lower inositol phosphates after its enzymatic hydrolysis and is also an effective tool to visualize the InsPx fingerprints and possible accumulations of inositol phosphates.
This paper presents a framework for numerical building validation enhancement based on detailed building specifications from in-situ measurements and evidence-based validation assessment undertaken on a detached sustainable lightweight building in a semi-arid climate. The validation process has been undergone in a set of controlled experiments – a free-float period, and steady-state internal temperatures. The validation was conducted for a complete year with a 1-min time step for the hourly indoor temperature and the variable refrigerant flow (VRF) energy consumption. The initial baseline model was improved by three series of validation steps per three different field measurements including thermal transmittance, glazing thermal and optical properties, and airtightness. Then, the accurate and validated model was used for building energy efficiency assessment in 12 regions of Morocco. This study aims to assess the effect of accurate building characteristics values on the numerical model enhancement. The initial CV(RMSE) and NMBE have improved respectively from 14.58 % and −11.23 %–7.85 % and 1.86 % for the indoor temperature. Besides, from 31.17 % to 14.37 %–20.57 % and 9.77 % for energy consumption. The findings demonstrate that the lightweight construction with the use of a variable refrigerant flow system could be energy efficient in the southern regions of Morocco.
In this paper, the time- and temperature-dependent cyclic ratchetting plasticity of the nickel-based alloy IN100 is experimentally investigated in strain-controlled experiments in the temperature range from 300 °C to 1050 °C. To this end, uniaxial material tests are performed with complex loading histories designed to activate phenomena as strain rate dependency, stress relaxation as well as the Bauschinger effect, cyclic hardening and softening, ratchetting and recovery from hardening. Plasticity models with different levels of complexity are presented that consider these phenomena, and a strategy is derived to determine the multitude of temperature-dependent material properties of the models in a step-by-step procedure based on sub-sets of experimental data of isothermal experiments. The models and the material properties are validated based on the results of non-isothermal experiments. A good description of the time- and temperature-dependent cyclic ratchetting plasticity of IN100 is obtained for isothermal as well as non-isothermal loading with models including ratchetting terms in the kinematic hardening law and the material properties obtained with the proposed strategy.
Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.