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One of the major challenges impeding the energy transition is the intermittency of solar and wind electricity generation due to their dependency on weather changes. The demand-side energy flexibility contributes considerably to mitigate the energy supply/demand imbalances resulting from external influences such as the weather. As one of the largest electricity consumers, the industrial enterprises present a high demand-side flexibility potential from their production processes and on-site energy assets. In this direction, methods are needed with a focus on enabling the energy flexibility and ensure an active participation of such enterprises in the electricity markets especially with variable prices of electricity. This paper presents a generic model library for an industrial enterprise implemented with optimal control for energy flexibility purposes. The components in the model library represent the typical technical units of an industrial enterprise on material, media, and energy flow levels with their operative constraints. A case study of a plastic manufacturing plant using the generic model library is also presented, in which the results of two simulation with different electricity prices are compared and the behavior of the model can be assessed. The results show that the model provides an optimal scheduling of the manufacturing system according to the variations in the electricity prices, and ensures an optimal control for utilities and energy systems needed for the production.
Solar energy plays a central role in the energy transition. Clouds generate locally large fluctuations in the generation output of photovoltaic systems, which is a major problem for energy systems such as microgrids, among others. For an optimal design of a power system, this work analyzed the variability using a spatially distributed sensor network at Stuttgart Airport. It has been shown that the spatial distribution partially reduces the variability of solar radiation. A tool was also developed to estimate the output power of photovoltaic systems using irradiation time series and assumptions about the photovoltaic sites. For days with high fluctuations of the estimated photovoltaic power, different energy system scenarios were investigated. It was found the approach can be used to have a more realistic representation of aggregated PV power taking spatial smoothing into account and that the resulting PV power generation profiles provide a good basis for energy system design considerations like battery sizing.
In recent years, light-weight cryptography has received a lot of attention. Many primitives suitable for resource-restricted hardware platforms have been proposed. In this paper, we present a cryptanalysis of the new stream cipher A2U2 presented at IEEE RFID 2011 [9] that has a key length of 56 bit. We start by disproving and then repairing an extremely efficient attack presented by Chai et al. [8], showing that A2U2 can be broken in less than a second in the chosen-plaintext case. We then turn our attention to the more challenging known-plaintext case and propose a number of attacks. A guess-and-determine approach combined with algebraic cryptanalysis yields an attack that requires about 249 internal guesses. We also show how to determine the 5-bit counter key and how to reconstruct the 56-bit key in about 238 steps if the attacker can freely choose the IV. Furthermore, we investigate the possibility of exploiting the knowledge of a “noisy keystream” by solving a Max-PoSSo problem. We conclude that the cipher needs to be repaired and point out a number of simple measures that would prevent the above attacks.
With the expansion of IoT devices in many aspects of our life, the security of such systems has become an important challenge. Unlike conventional computer systems, any IoT security solution should consider the constraints of these systems such as computational capability, memory, connectivity, and power consumption limitations. Physical Unclonable Functions (PUFs) with their special characteristics were introduced to satisfy the security needs while respecting the mentioned constraints. They exploit the uncontrollable and reproducible variations of the underlying component for security applications such as identification, authentication, and communication security. Since IoT devices are typically low cost, it is important to reuse existing elements in their hardware (for instance sensors, ADCs, etc.) instead of adding extra costs for the PUF hardware. Micro-electromechanical system (MEMS) devices are widely used in IoT systems as sensors and actuators. In this thesis, a comprehensive study of the potential application of MEMS devices as PUF primitives is provided. MEMS PUF leverages the uncontrollable variations in the parameters of MEMS elements to derive secure keys for cryptographic applications. Experimental and simulation results show that our proposed MEMS PUFs are capable of generating enough entropy for a complex key generation, while their responses show low fluctuations in different environmental conditions.
Keeping in mind that the PUF responses are prone to change in the presence of noise and environmental variations, it is critical to derive reliable keys from the PUF and to use the maximum entropy at the same time. In the second part of this thesis, we elaborate on different key generation schemes and their advantages and drawbacks. We propose the PUF output positioning (POP) and integer linear programming (ILP) methods, which are novel methods for grouping the PUF outputs in order to maximize the extracted entropy. To implement these methods, the key enrollment and key generation algorithms are presented. The proposed methods are then evaluated by applying on the responses of the MEMS PUF, where it can be practically shown that the proposed method outperforms other existing PUF key generation methods.
The final part of this thesis is dedicated to the application of the MEMS PUF as a security solution for IoT systems. We select the mutual authentication of IoT devices and their backend system, and propose two lightweight authentication protocols based on MEMS PUFs. The presented protocols undergo a comprehensive security analysis to show their eligibility to be used in IoT systems. As the result, the output of this thesis is a lightweight security solution based on MEMS PUFs, which introduces a very low overhead on the cost of the hardware.
The number of use cases for autonomous vehicles is increasing day by day especially in commercial applications. One important application of autonomous vehicles can be found within the parcel delivery section. Here, autonomous cars can massively help to reduce delivery efforts and time by supporting the courier actively. One important component of course is the autonomous vehicle itself. Nevertheless, beside the autonomous vehicle, a flexible and secure communication architecture also is a crucial key component impacting the overall performance of such system since it is required to allow continuous interactions between the vehicle and the other components of the system. The communication system must provide a reliable and secure architecture that is still flexible enough to remain practical and to address several use cases. In this paper, a robust communication architecture for such autonomous fleet-based systems is proposed. The architecture provides a reliable communication between different system entities while keeping those communications secure. The architecture uses different technologies such as Bluetooth Low Energy (BLE), cellular networks and Low Power Wide Area Network (LPWAN) to achieve its goals.
The desire to connect more and more devices and to make them more intelligent and more reliable, is driving the needs for the Internet of Things more than ever. Such IoT edge systems require sound security measures against cyber-attacks, since they are interconnected, spatially distributed, and operational for an extended period of time. One of the most important requirements for the security in many industrial IoT applications is the authentication of the devices. In this paper, we present a mutual authentication protocol based on Physical Unclonable Functions, where challenge-response pairs are used for both device and server authentication. Moreover, a session key can be derived by the protocol in order to secure the communication channel. We show that our protocol is secure against machine learning, replay, man-in-the-middle, cloning, and physical attacks. Moreover, it is shown that the protocol benefits from a smaller computational, communication, storage, and hardware overhead, compared to similar works.
In recent years, Physical Unclonable Functions (PUFs) have gained significant attraction in the Internet of Things (IoT) for security applications such as cryptographic key generation and entity authentication. PUFs extract the uncontrollable production characteristics of physical devices to generate unique fingerprints for security applications. One common approach for designing PUFs is exploiting the intrinsic features of sensors and actuators such as MEMS elements, which typically exist in IoT devices. This work presents the Cantilever-PUF, a PUF based on a specific MEMS device – Aluminum Nitride (AlN) piezoelectric cantilever. We show the variations of electrical parameters of AlN cantilevers such as resonance frequency, electrical conductivity, and quality factor, as a result of uncontrollable manufacturing process variations. These variations, along with high thermal and chemical stability, and compatibility with silicon technology, makes AlN cantilever a decent candidate for PUF design. We present a cantilever design, which magnifies the effect of manufacturing process variations on electrical parameters. In order to verify our findings, the simulation results of the Monte Carlo method are provided. The results verify the eligibility of AlN cantilever to be used as a basic PUF device for security applications. We present an architecture, in which the designed Cantilever-PUF is used as a security anchor for PUF-enabled device authentication as well as communication encryption.
In recent years, physically unclonable functions (PUFs) have gained significant attraction in IoT security applications, such as cryptographic key generation and entity authentication. PUFs extract the uncontrollable production characteristics of different devices to generate unique fingerprints for security applications. When generating PUF-based secret keys, the reliability and entropy of the keys are vital factors. This study proposes a novel method for generating PUF-based keys from a set of measurements. Firstly, it formulates the group-based key generation problem as an optimization problem and solves it using integer linear programming (ILP), which guarantees finding the optimum solution. Then, a novel scheme for the extraction of keys from groups is proposed, which we call positioning syndrome coding (PSC). The use of ILP as well as the introduction of PSC facilitates the generation of high-entropy keys with low error correction costs. These new methods have been tested by applying them on the output of a capacitor network PUF. The results confirm the application of ILP and PSC in generating high-quality keys.
Physical unclonable functions (PUFs) are increasingly generating attention in the field of hardware-based security for the Internet of Things (IoT). A PUF, as its name implies, is a physical element with a special and unique inherent characteristic and can act as the security anchor for authentication and cryptographic applications. Keeping in mind that the PUF outputs are prone to change in the presence of noise and environmental variations, it is critical to derive reliable keys from the PUF and to use the maximum entropy at the same time. In this work, the PUF output positioning (POP) method is proposed, which is a novel method for grouping the PUF outputs in order to maximize the extracted entropy. To achieve this, an offset data is introduced as helper data, which is used to relax the constraints considered for the grouping of PUF outputs, and deriving more entropy, while reducing the secret key error bits. To implement the method, the key enrollment and key generation algorithms are presented. Based on a theoretical analysis of the achieved entropy, it is proven that POP can maximize the achieved entropy, while respecting the constraints induced to guarantee the reliability of the secret key. Moreover, a detailed security analysis is presented, which shows the resilience of the method against cyber-security attacks. The findings of this work are evaluated by applying the method on a hybrid printed PUF, where it can be practically shown that the proposed method outperforms other existing group-based PUF key generation methods.
Steroid hormones (SHs) are a rising concern due to their high bioactivity, ubiquitous nature, and prolonged existence as a micropollutants in water, they pose a potential risk to both human health and the environment, even at low concentrations. Estrogens, progesterone, and testosterone are the three important types of steroids essential for human development and maintaining multiorgan balance, are focus to this concern. These steroid hormones originate
from various sources, including human and livestock excretions, veterinary medications, agricultural runoff, and pharmaceuticals, contributing to their presence in the environment. According to the recommendation of WHO, the guidance value for estradiol (E2) is 1 ng/L. There are several methods been attempted to remove the SH micropollutant by conventional water and wastewater technologies which are still under research. Among the various methods, electrochemical membrane reactor (EMR) is one of the emerging technologies that can address the challenge of insufficient SHs removal from the aquatic environment by conventional treatment. The degradation of SHs can be significantly influenced by various factors when treated with EMR.
In this project, the removal of SH and the important mechanism for the removal using carbon nanotube CNT-EMR is studied and the efficiency of CNT-EMR in treating the SH micropollutant is identified. By varying different parameters this experiment is carried out with the (PES-CNTs) ultrafiltration membrane. The study is carried out depending upon the SH removal based on the limiting factor such as cell voltage, flux, temperature, concentration, and type of the SH.
This thesis focuses on the development and implementation of a Datagram Transport Layer Security (DTLS) communication framework within the ns-3 network simulator, specifically targeting the LoRaWAN model network. The primary aim is to analyse the behaviour and performance of DTLS protocols across different network conditions within a LoRaWAN context. The key aspects of this work include the following.
Utilization of ns-3: This thesis leverages ns-3’s capabilities as a powerful discrete event network simulator. This platform enables the emulation of diverse network environments, characterized by varying levels of latency, packet loss, and bandwidth constraints.
Emulation of Network Challenges: The framework specifically addresses unique challenges posed by certain network configurations, such as duty cycle limitations. These constraints, which limit the time allocated for data transmission by each device, are crucial in understanding the real-world performance of DTLS protocols.
Testing in Multi-client-server Scenarios: A significant feature of this framework is its ability to test DTLS performance in complex scenarios involving multiple clients and servers. This is vital for assessing the behaviour of a protocol under realistic network conditions.
Realistic Environment Simulation: By simulating challenging network conditions, such as congestion, limited bandwidth, and resource constraints, the framework provides a realistic environment for thorough evaluation. This allows for a comprehensive analysis of DTLS in terms of security, performance, and scalability.
Overall, this thesis contributes to a deeper understanding of DTLS protocols by providing a robust tool for their evaluation under various and challenging network conditions.
Global energy demand is still on an increase during the last decade, with a lot of impact on the climate change due to the intensive use of conventional fossil-based fuels power plants to cover this demand. Most recently, leaders of the globe met in 2015 to come out with the Paris Agreement, stating that the countries will start to take a more responsible and effective behaviour toward the global warming and climate change issues. Many studies have discussed how the future energy system will look like with respecting the countries’ targets and limits of greenhouse gases and their CO2 emissions. However, these studies rarely discussed the industry sector in detail even though it is one of the major role players in the energy sector. Moreover, many studies have simulated and modelled the energy system with huge jumps of intervals in terms of years and environmental goals. In the first part of this study, a model will be developed for the German electrical grid with high spatial and temporal resolutions and different scenarios of it will be analysed meticulously on shorter periods (annual optimization), with different flexibilities and used technologies and degrees of innovations within each scenario. Moreover, the challenge in this research is to adequately map the diverse and different characteristics of the medium-sized industrial sector. In order to be able to take a first step in assessing the relevance of the industrial sector in Germany for climate protection goals, the industrial sector will be mapped in PyPSA-Eur (an open-source model data set of the European energy system at the level of the transmission network) by detailing the demand for different types of industry and assigning flexibilities to the industrial types. Synthetically generated load profiles of various industrial types are available. Flexibilities in the industrial sector are described by the project partner Fraunhofer IPA in the GaIN project and can be used. Using a scenario analysis, the development of the industrial sector and the use of flexibilities are then to be assessed quantitatively.
This paper will introduce the open-source model MyPyPSA-Ger, a myopic optimization model developed to represent the German energy system with a detailed mapping of the electricity sector, on a highly disaggregated level, spatially and temporally, with regional differences and investment limitations. Furthermore, this paper will give new outlooks on the German federal government 2050 emissions goals of the electricity sector to become greenhouse gas neutral by proposing new CO2 allowance strategies. Moreover, the regional differences in Germany will be discussed, their role and impact on the energy transition, and which regions and states will drive the renewable energy utilization forward.
Following a scenario-based analysis, the results point out the major keystones of the energy transition path from 2020 to 2050. Solar, onshore wind, and gas-fired power plants will play a fundamental role in the future electricity systems. Biomass, run of river, and offshore wind technologies will be utilized in the system as base-load generation technologies. Solar and onshore wind will be installed almost everywhere in Germany. However, due to the nature of Germany’s weather and geographical features, the southern and northern regions will play a more important role in the energy transition.
Higher CO2 allowance costs will help achieve the 1.5-degree-target of the electricity system and will allow for a rapid transition. Moreover, the more expensive, and the earlier the CO2 tax is applied to the system, the less it will cost for the energy transition, and the more emissions will be saved throughout the transition period. An earlier phase-out of coal power plants is not necessary with high CO2 taxes, due to the change in power plant’s unit commitment, as they prioritize gas before coal power plants. Having moderate to low CO2 allowance cost or no clear transition policy will be more expensive and the CO2 budget will be exceeded. Nonetheless, even with no policy, renewables still dominate the energy mix of the future.
However, maintaining the maximum historical installation rates of both national and regional levels, with the current emissions reduction strategy, will not be enough to reach the level of climate-neutral electricity system. Therefore, national and regional installation requirements to achieve the federal government emission reduction goals are determined. Energy strategies and decision makers will have to resolve great challenges in order to stay in line with the 1.5-degree-target.
Most recently, the federal government in Germany published new climate goals in order reach climate neutrality by 2045. This paper demonstrates a path to a cost optimal energy supply system for the German power grid until the year 2050. With special regard to regionality, the system is based on yearly myopic optimization with the required energy system transformation measures and the associated system costs. The results point out, that energy storage systems (ESS) are fundamental for renewables integration in order to have a feasible energy transition. Moreover, the investment in storage technologies increased the usage of the solar and wind technologies. Solar energy investments were highly accompanied with the installation of short-term battery storage. Longer-term storage technologies, such as H2, were accompanied with high installations of wind technologies. The results pointed out that hydrogen investments are expected to overrule short-term batteries if their cost continues to decrease sharply. Moreover, with a strong presence of ESS in the energy system, biomass energy is expected to be completely ruled out from the energy mix. With the current emission reduction strategy and without a strong presence of large scale ESS into the system, it is unlikely that the Paris agreement 2° C target by 2050 will be achieved, let alone the 1.5° C.
With recent developments in the Ukrainian-Russian conflict, many are discussing about Germany’s dependency on fossil fuel imports in its energy system, and how can the country proceed with reducing that dependency. With its wide-ranging consumption sectors, the electricity sector comes as the perfect choice to start with. Recent reports showed that the German federal government is already intending to have a fully renewable electricity by 2035 while exploiting all possible clean power options. This was published in the federal government’s climate emergency program (Easter Package) in early 2022. The aim of this package is to initiate a rapid transition and decarbonization of the electricity sector. The Easter Package expects an enormous growth of renewable energies to a completely new level, with already at least 80% renewable gross energy consumption, with extensive and broad deployment of different generation technologies on various scales. This paper will discuss this ambitious plan and outline some insights into this huge and rapidly increasing step, and show how much will Germany need in order to achieve this huge milestone towards a fully green supply of the electricity sector. Different scenarios and shares of renewables will be investigated in order to elaborate on preponed climate-neutral goal of the electricity sector by 2035. The results pointed out some promising aspects in achieving a 100% renewable power, with massive investments in both generation and storage technologies.
An import ban of Russian energy sources to Germany is currently being increasingly discussed. We want to support the discussion by showing a way how the electricity system in Germany can manage low energy imports in the short term and which measures are necessary to still meet the climate protection targets. In this paper, we examine the impact of a complete stop of Russian fossil fuel imports on the electricity sector in Germany, and how this will affect the climate coals of an earlier coal phase-out and climate neutrality by 2045.
Following a scenario-based analysis, the results gave a point of view on how much would be needed to completely rely on the scarce non-renewable energy resources in Germany. Huge amounts of investments would be needed in order to ensure a secure supply of electricity, in both generation energy sources (RES) and energy storage systems (ESS). The key findings are that a rapid expansion of renewables and storage technologies will significantly reduce the dependence of the German electricity system on energy imports. The huge integration of renewable energy does not entail any significant imports of the energy sources natural gas, hard coal, and mineral oil, even in the long term. The results showed that a ban on fossil fuel imports from Russia outlines huge opportunities to go beyond the German government's climate targets, where the 1.5-degree-target is achieved in the electricity system.
Method and system for extractin metal and oxygen from powdered metal oxides (EP000004170066A2)
(2023)
A method for extracting metal and oxygen from powdered metal oxides in electrolytic cell is proposed, the electrolytic cell comprising a container, a cathode, an anode and an oxygen-ion-conducting membrane, the method comprising providing a solid oxygen ion conducting electrolyte powder into a container, providing a feedstock comprising at least one metal oxide in powdered form into the container, applying an electric potential across the cathode and the anode, the cathode being in communication with the electrolyte powder and the anode being in communication with the membrane in communication with the electrolyte powder, such that at least one respective metallic species of the at least one metal oxide is reduced at the cathode and oxygen is oxidized at the anode to form molecular oxygen, wherein the potential across the cathode and the anode is greater than the dissociation potential of the at least one metal oxide and less than the dissociation potential of the solid electrolyte powder and the membrane.
To deal with frequent power outages in developing countries, people turn to solutions like uninterruptible power supply (UPS), which stores electric energy during normal operating hours and use it to meet energy needs during rolling blackout intervals. Locally produced UPSs of poorer power quality are widely accessible in the marketplaces, and they have a negative impact on power quality. The charging and discharging of the batteries in these UPSs generate significant amount of power losses in weak grid environments. The Smart-UPS is our proposed smart energy metering (SEM) solution for low voltage consumers that is provided by the distribution company. It does not require batteries, therefore there is no power loss or harmonic distortion due to corresponding charging and discharging. Through load flow and harmonic analysis of both traditional UPS and Smart-UPS systems on ETAP, this paper examines their impact on the harmonics and stability of the distribution grid. The simulation results demonstrate that Smart-UPS can assist fixing power quality issues in a developing country like Pakistan by providing cleaner energy than the battery-operated traditional UPSs.
Due to its potential in improving the efficiency of energy supply, smart energy metering (SEM) has become an area of interest with the surge in Internet of Things (IoT). SEM entails remote monitoring and control of the sensors and actuators associated with the energy supply system. This provides a flexible platform to conceive and implement new data driven Demand Side Management (DSM) mechanisms. The IoT enablement allows the data to be gathered and analyzed at requisite granularity. In addition to efficient use of energy resources and provisioning of power, developing countries face an additional challenge of temporal mismatch in generation capacity and load factors. This leads to widespread deployment of inefficient and expensive Uninterruptible Power Supply (UPS) solutions for limited power provisioning during resulting blackouts. Our proposed “Soft-UPS” allows dynamic matching of load and generation through a combination of managed curtailment. This eliminates inefficiencies in the energy and power value chain and allows a data-driven approach to solving a widespread problem in developing countries, simultaneously reducing both upfront and running costs of conventional UPS and storage. A scalable and modular platform is proposed and implemented in this paper. The architecture employs “WiMODino” using LoRaWAN with a “Lite Gateway” and SQLite repository for data storage. Role based access to the system through an android application has also been demonstrated for monitoring and control.
Following their success in visual recognition tasks, Vision Transformers(ViTs) are being increasingly employed for image restoration. As a few recent works claim that ViTs for image classification also have better robustness properties, we investigate whether the improved adversarial robustness of ViTs extends to image restoration. We consider the recently proposed Restormer model, as well as NAFNet and the "Baseline network" which are both simplified versions of a Restormer. We use Projected Gradient Descent (PGD) and CosPGD for our robustness evaluation. Our experiments are performed on real-world images from the GoPro dataset for image deblurring. Our analysis indicates that contrary to as advocated by ViTs in image classification works, these models are highly susceptible to adversarial attacks. We attempt to find an easy fix and improve their robustness through adversarial training. While this yields a significant increase in robustness for Restormer, results on other networks are less promising. Interestingly, we find that the design choices in NAFNet and Baselines, which were based on iid performance, and not on robust generalization, seem to be at odds with the model robustness.
State-of-the-art models for pixel-wise prediction tasks such as image restoration, image segmentation, or disparity estimation, involve several stages of data resampling, in which the resolution of feature maps is first reduced to aggregate information and then sequentially increased to generate a high-resolution output. Several previous works have investigated the effect of artifacts that are invoked during downsampling and diverse cures have been proposed that facilitate to improve prediction stability and even robustness for image classification. However, equally relevant, artifacts that arise during upsampling have been less discussed. This is significantly relevant as upsampling and downsampling approaches face fundamentally different challenges. While during downsampling, aliases and artifacts can be reduced by blurring feature maps, the emergence of fine details is crucial during upsampling. Blurring is therefore not an option and dedicated operations need to be considered. In this work, we are the first to explore the relevance of context during upsampling by employing convolutional upsampling operations with increasing kernel size while keeping the encoder unchanged. We find that increased kernel sizes can in general improve the prediction stability in tasks such as image restoration or image segmentation, while a block that allows for a combination of small-size kernels for fine details and large-size kernels for artifact removal and increased context yields the best results.
With the surge in global data consumption with proliferation of Internet of Things (IoT), remote monitoring and control is increasingly becoming popular with a wide range of applications from emergency response in remote regions to monitoring of environmental parameters. Mesh networks are being employed to alleviate a number of issues associated with single-hop communication such as low area coverage, reliability, range and high energy consumption. Low-power Wireless Personal Area Networks (LoWPANs) are being used to help realize and permeate the applicability of IoT. In this paper, we present the design and test of IEEE 802.15.4-compliant smart IoT nodes with multi-hop routing. We first discuss the features of the software stack and design choices in hardware that resulted in high RF output power and then present field test results of different baseline network topologies in both rural and urban settings to demonstrate the deployability and scalability of our solution.
Modeling of Random Variations in a Switched Capacitor Circuit based Physically Unclonable Function
(2020)
The Internet of Things (IoT) is expanding to a wide range of fields such as home automation, agriculture, environmental monitoring, industrial applications, and many more. Securing tens of billions of interconnected devices in the near future will be one of the biggest challenges. IoT devices are often constrained in terms of computational performance, area, and power, which demand lightweight security solutions. In this context, hardware-intrinsic security, particularly physically unclonable functions (PUFs), can provide lightweight identification and authentication for such devices. In this paper, random capacitor variations in a switched capacitor PUF circuit are used as a source of entropy to generate unique security keys. Furthermore, a mathematical model based on the ordinary least square method is developed to describe the relationship between random variations in capacitors and the resulting output voltages. The model is used to filter out systematic variations in circuit components to improve the quality of the extracted secrets.
BACKGROUND
Various neutral and alkaline peptidases are commercially available for use in protein hydrolysis under neutral to alkaline conditions. However, the hydrolysis of proteins under acidic conditions by applying fungal aspartic peptidases (FAPs) has not been investigated in depth so far. The aim of this study, thus, was to purify a FAP from the commercial enzyme preparation, ROHALASE® BXL, determine its biochemical characteristics, and investigate its application for the hydrolysis of food and animal feed proteins under acidic conditions.
RESULTS
A Trichoderma reesei derived FAP, with an apparent molecular mass of 45.8 kDa (sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SDS-PAGE) was purified 13.8-fold with a yield of 37% from ROHALASE® BXL. The FAP was identified as an aspartate protease (UniProt ID: G0R8T0) by inhibition and nano-LC-ESI-MS/MS studies. The FAP showed the highest activity at 50°C and pH 4.0. Monovalent cations, organic solvents, and reducing agents were tolerated well by the FAP. The FAP underwent an apparent competitive product inhibition by soy protein hydrolysate and whey protein hydrolysate with apparent Ki-values of 1.75 and 30.2 mg*mL−1, respectively. The FAP showed promising results in food (soy protein isolate and whey protein isolate) and animal feed protein hydrolyses. For the latter, an increase in the soluble protein content of 109% was noted after 30 min.
CONCLUSION
Our results demonstrate the applicability of fungal aspartic endopeptidases in the food and animal feed industry. Efficient protein hydrolysis of industrially relevant substrates such as acidic whey or animal feed proteins could be conducted by applying fungal aspartic peptidases. © 2022 Society of Chemical Industry.
A novel peptidyl-lys metalloendopeptidase (Tc-LysN) from Tramates coccinea was recombinantly expressed in Komagataella phaffii using the native pro-protein sequence. The peptidase was secreted into the culture broth as zymogen (~38 kDa) and mature enzyme (~19.8 kDa) simultaneously. The mature Tc-LysN was purified to homogeneity with a single step anion-exchange chromatography at pH 7.2. N-terminal sequencing using TMTpro Zero and mass spectrometry of the mature Tc-LysN indicated that the pro-peptide was cleaved between the amino acid positions 184 and 185 at the Kex2 cleavage site present in the native pro-protein sequence. The pH optimum of Tc-LysN was determined to be 5.0 while it maintained ≥60% activity between pH values 4.5—7.5 and ≥30% activity between pH values 8.5—10.0, indicating its broad applicability. The temperature maximum of Tc-LysN was determined to be 60 °C. After 18 h of incubation at 80 °C, Tc-LysN still retained ~20% activity. Organic solvents such as methanol and acetonitrile, at concentrations as high as 40% (v/v), were found to enhance Tc-LysN’s activity up to ~100% and ~50%, respectively. Tc-LysN’s thermostability, ability to withstand up to 8 M urea, tolerance to high concentrations of organic solvents, and an acidic pH optimum make it a viable candidate to be employed in proteomics workflows in which alkaline conditions might pose a challenge. The nano-LC-MS/MS analysis revealed bovine serum albumin (BSA)’s sequence coverage of 84% using Tc-LysN which was comparable to the sequence coverage of 90% by trypsin peptides.
A systematic toxicological analysis procedure using high-performance thin layer chromatography in combination with fibre optical scanning densitometry for identification of drugs in biological samples is presented. Two examples illustrate the practicability of the technique. First, the identification of a multiple intake of analgesics: codeine, propyphenazone, tramadol, flupirtine and lidocaine, and second, the detection of the sedative diphenhydramine. In both cases, authentic urine specimens were used. The identifications were carried out by an automatic measurement and computer-based comparison of in situ UV spectra with data from a compiled library of reference spectra using the cross-correlation function. The technique allowed a parallel recording of chromatograms and in situ UV spectra in the range of 197–612 nm. Unlike the conventional densitometry, a dependency of UV spectra by concentration of substance in a range of 250–1000 ng/spot was not observed.
This paper describes the Sweaty II humanoid adult size robot trying to qualify for the RoboCup 2018 adult size humanoid competition. Sweaty came 2nd in RoboCup 2017 adult size league. The main characteristics of Sweaty are described in the Team Description Paper 2017. The improvements that have been made or are planned to be implemented for RoboCup 2018 are described in this paper.
Soccer simulation league is one of the founding leagues of RoboCup. In this paper we discuss the past, present and planned future achievements and changes. Also we summarize the connections and inter-league achievements of this league and provide an overview of the community contributions that made this league successful.
Blockchain-IIoT integration into industrial processes promises greater security, transparency, and traceability. However, this advancement faces significant storage and scalability issues with existing blockchain technologies. Each peer in the blockchain network maintains a full copy of the ledger which is updated through consensus. This full replication approach places a burden on the storage space of the peers and would quickly outstrip the storage capacity of resource-constrained IIoT devices. Various solutions utilizing compression, summarization or different storage schemes have been proposed in literature. The use of cloud resources for blockchain storage has been extensively studied in recent years. Nonetheless, block selection remains a substantial challenge associated with cloud resources and blockchain integration. This paper proposes a deep reinforcement learning (DRL) approach as an alternative to solving the block selection problem, which involves identifying the blocks to be transferred to the cloud. We propose a DRL approach to solve our problem by converting the multi-objective optimization of block selection into a Markov decision process (MDP). We design a simulated blockchain environment for training and testing our proposed DRL approach. We utilize two DRL algorithms, Advantage Actor-Critic (A2C), and Proximal Policy Optimization (PPO) to solve the block selection problem and analyze their performance gains. PPO and A2C achieve 47.8% and 42.9% storage reduction on the blockchain peer compared to the full replication approach of conventional blockchain systems. The slowest DRL algorithm, A2C, achieves a run-time 7.2 times shorter than the benchmark evolutionary algorithms used in earlier works, which validates the gains introduced by the DRL algorithms. The simulation results further show that our DRL algorithms provide an adaptive and dynamic solution to the time-sensitive blockchain-IIoT environment.
An Overview of Technologies for Improving Storage Efficiency in Blockchain-Based IIoT Applications
(2022)
Since the inception of blockchain-based cryptocurrencies, researchers have been fascinated with the idea of integrating blockchain technology into other fields, such as health and manufacturing. Despite the benefits of blockchain, which include immutability, transparency, and traceability, certain issues that limit its integration with IIoT still linger. One of these prominent problems is the storage inefficiency of the blockchain. Due to the append-only nature of the blockchain, the growth of the blockchain ledger inevitably leads to high storage requirements for blockchain peers. This poses a challenge for its integration with the IIoT, where high volumes of data are generated at a relatively faster rate than in applications such as financial systems. Therefore, there is a need for blockchain architectures that deal effectively with the rapid growth of the blockchain ledger. This paper discusses the problem of storage inefficiency in existing blockchain systems, how this affects their scalability, and the challenges that this poses to their integration with IIoT. This paper explores existing solutions for improving the storage efficiency of blockchain–IIoT systems, classifying these proposed solutions according to their approaches and providing insight into their effectiveness through a detailed comparative analysis and examination of their long-term sustainability. Potential directions for future research on the enhancement of storage efficiency in blockchain–IIoT systems are also discussed.
Linux and Linux-based operating systems have been gaining more popularity among the general users and among developers. Many big enterprises and large companies are using Linux for servers that host their websites, some even require their developers to have knowledge about Linux OS. Even in embedded systems one can find many Linux-based OS that run them. With its increasing popularity, one can deduce the need to secure such a system that many personnel rely on, be it to protect the data that it stores or to protect the integrity of the system itself, or even to protect the availability of the services it offers. Many researchers and Linux enthusiasts have been coming up with various ways to secure Linux OS, however new vulnerabilities and new bugs are always found, by malicious attackers, with every update or change, which calls for the need of more ways to secure these systems.
This Thesis explores the possibility and feasibility of another way to secure Linux OS, specifically securing the terminal of such OS, by altering the commands of the terminal, getting in the way of attackers that have gained terminal access and delaying, giving more time for the response teams and for forensics to stop the attack, minimize the damage, restore operations, and to identify collect and store evidence of the cyber-attack. This research will discuss the advantages and disadvantages of various security measures and compare and contrast with the method suggested in this research.
This research is significant because it paints a better picture of what the state of the art of Linux and Linux-based operating systems security looks like, and it addresses the concerns of security enthusiasts, while exploring new uncharted area of security that have been looked at as a not so significant part of protecting the OSes out of concern of the various limitations and problems it entails. This research will address these concerns while exploring few ways to solve them, as well as addressing the ideal areas and situations in which the proposed method can be used, and when would such method be more of a burden than help if used.
Soiling is an important issue in the renewable energy sector since it can result in significant yield losses, especially in regions with higher pollution or dust levels. To mitigate the impact of soiling on photovoltaic (PV) plants, it is essential to regularly monitor and clean the panels, as well as develop accurate soiling predictions that can affect cleaning strategies and enhance the overall performance of PV power plants. This research focuses on the problem of soiling loss in photovoltaic power plants and the potential to improve the accuracy of soiling predictions. The study examines how soiling can affect the efficiency and productivity of the modules and how to measure and predict soiling using machine learning (ML) algorithms. The research includes analyzing real data from large-scale ground-mounted PV sites and comparing different soiling measurement methods. It was observed that there were some deviations in the real soiling loss values compared to the expected values for some projects in southern Spain, thus, the main goal of this work is to develop machine learning models that could predict the soiling more accurately. The developed models have a low mean square error (MSE), indicating the accuracy and suitability of the models to predict the soiling rates. The study also investigates the impact of different cleaning strategies on the performance of PV power plants and provides a powerful application to predict both the soiling and the number of cleaning cycles.
Passive solar elements for both direct and indirect gains, are systems used to maintain a comfortable living environment while saving energy, especially in the building energy retrofit and adaptation process. Sunspaces, thermal mass and glazing area and orientation have been often used in the past to guarantee adequate indoor conditions when mechanical devices were not available. After a period of neglect, nowadays they are again considered as appropriate systems to help face environmental issues in the building sector, and both international and national legislation takes into consideration the possibility of including them in the building planning tools, also providing economic incentives. Their proper design needs dynamic simulation, often difficult to perform and time consuming. Moreover, results generally suffer from several uncertainties, so quasi steady-state procedures are often used in everyday practice with good results, but some corrections are still needed. In this paper, a comparative analysis of different solutions for the construction of verandas in an existing building is presented, following the procedure provided by the slightly modified and improved Standard EN ISO 13790:2008. Advantages and disadvantages of different configurations considering thermal insulation, windows typology and mechanical ventilation systems are discussed and a general intervention strategy is proposed. The aim is to highlight the possibility of using sunspaces in order to increase the efficiency of the existing building stock, considering ease of construction and economic viability.
Energy Performance of Verandas in the Building Retrofit Process (PDF Download Available). Available from: https://www.researchgate.net/publication/303093420_Energy_Performance_of_Verandas_in_the_Building_Retrofit_Process [accessed Jul 5, 2017].
Germany was considered the world's export champion for a long time, until it was overtaken by China in 2009. Both nations provide officially supported export credits to national exporting organizations, but the two systems operate differently. German export credit guarantees serve as a substitute when the private market is unable to assume the risks of exporting companies. The German Export Credit Agency Euler Hermes is responsible for processing applications on behalf of the Federal Government. China belongs to the largest providers of export finance with the institutions China EXIM and Sinosure. While Germany is bound by the OECD consensus, which defines the level playing field, Chinese export credit agencies have greater flexibility not being bound by international rules or agreements.
Femtosecond (fs) time-resolved magneto-optics is applied to investigate laser-excited ultrafast dynamics of one-dimensional nickel gratings on fused silica and silicon substrates for a wide range of periodicities Λ = 400–1500 nm. Multiple surface acoustic modes with frequencies up to a few tens of GHz are generated. Nanoscale acoustic wavelengths Λ/n have been identified as nth-spatial harmonics of Rayleigh surface acoustic wave (SAW) and surface skimming longitudinal wave (SSLW), with acoustic frequencies and lifetimes being in agreement with theoretical calculations. Resonant magnetoelastic excitation of the ferromagnetic resonance (FMR) by SAW’s third spatial harmonic, and, most interestingly fingerprints of the parametric resonance at 1/2 SAW frequency have been observed. Numerical solutions of Landau–Lifshitz–Gilbert (LLG) equation magnetoelastically driven by complex polychromatic acoustic fields quantitatively reproduce all resonances at once. Thus, our results provide a solid experimental and theoretical base for a quantitative understanding of ultrafast fs-laser-driven magnetoacoustics and tailoring the magnetic-grating-based metasurfaces at the nanoscale.
AI-based Ground Penetrating Radar Signal Processing for Thickness Estimation of Subsurface Layers
(2023)
This thesis focuses on the estimation of subsurface layer thickness using Ground Penetrating Radar (GPR) A-scan and B-scan data through the application of neural networks. The objective is to develop accurate models capable of estimating the thickness of up to two subsurface layers.
Two different approaches are explored for processing the A-scan data. In the first approach, A-scans are compressed using Principal Component Analysis (PCA), and a regression feedforward neural network is employed to estimate the layers’ thicknesses. The second approach utilizes a regression one-dimensional Convolutional Neural Network (1-D CNN) for the same purpose. Comparative analysis reveals that the second approach yields superior results in terms of accuracy.
Subsequently, the proposed 1-D CNN architecture is adapted and evaluated for Step Frequency Continuous Wave (SFCW) radar, expanding its applicability to this type of radar system. The effectiveness of the proposed network in estimating subsurface layer thickness for SFCW radar is demonstrated.
Furthermore, the thesis investigates the utilization of GPR B-scan images as input data for subsurface layer thickness estimation. A regression CNN is employed for this purpose, although the results achieved are not as promising as those obtained with the 1-D CNN using A-scan data. This disparity is attributed to the limited availability of B-scan data, as B-scan generation is a resource-intensive process.
In this paper, a concept for an anthropomorphic replacement hand cast with silicone with an integrated sensory feedback system is presented. In order to construct the personalized replacement hand, a 3D scan of a healthy hand was used to create a 3D-printed mold using computer-aided design (CAD). To allow for movement of the index and middle fingers, a motorized orthosis was used. Information about the applied force for grasping and the degree of flexion of the fingers is registered using two pressure sensors and one bending sensor in each movable finger. To integrate the sensors and additional cavities for increased flexibility, the fingers were cast in three parts, separately from the rest of the hand. A silicone adhesive (Silpuran 4200) was examined to combine the individual parts afterwards. For this, tests with different geometries were carried out. Furthermore, different test series for the secure integration of the sensors were performed, including measurements of the registered information of the sensors. Based on these findings, skin-toned individual fingers and a replacement hand with integrated sensors were created. Using Silpuran 4200, it was possible to integrate the needed cavities and to place the sensors securely into the hand while retaining full flexion using a motorized orthosis. The measurements during different loadings and while grasping various objects proved that it is possible to realize such a sensory feedback system in a replacement hand. As a result, it can be stated that the cost-effective realization of a personalized, anthropomorphic replacement hand with an integrated sensory feedback system is possible using 3D scanning and 3D printing. By integrating smaller sensors, the risk of damaging the sensors through movement could be decreased.
Linear acceleration is a key performance determinant and major training component of many sports. Although extensive research about lower limb kinetics and kinematics is available, consistent definitions of distinctive key body positions, the underlying mechanisms and their related movement strategies are lacking. The aim of this ‘Method and Theoretical Perspective’ article is to introduce a conceptual framework which classifies the sagittal plane ‘shin roll’ motion during accelerated sprinting. By emphasising the importance of the shin segment’s orientation in space, four distinctive key positions are presented (‘shin block’, ‘touchdown’, ‘heel lock’ and ‘propulsion pose’), which are linked by a progressive ‘shin roll’ motion during swing-stance transition. The shin’s downward tilt is driven by three different movement strategies (‘shin alignment’, ‘horizontal ankle rocker’ and ‘shin drop’). The tilt’s optimal amount and timing will contribute to a mechanically efficient acceleration via timely staggered proximal-to-distal power output. Empirical data obtained from athletes of different performance levels and sporting backgrounds are required to verify the feasibility of this concept. The framework presented here should facilitate future biomechanical analyses and may enable coaches and practitioners to develop specific training programs and feedback strategies to provide athletes with a more efficient acceleration technique.
The central purpose of this paper is to present a novel framework supporting the specification and the implementation of media streaming services using XML and Java Media Framework (JMF). It provides an integrated service development environment comprising of a streaming service model, a service specification language and several implementation and retrieval tools. Our approach is based on a clear separation of a streaming service specification, and its implementation by a distributed JMF application and can be used for different streaming paradigms, e.g. push and pull services.
The central purpose of this paper is to present a novel framework supporting the specification, the implementation and retrieval of media streaming services. It provides an integrated service development environment comprising of a streaming service model, a service specification language and several implementation and retrieval tools. Our approach is based on a clear separation of a streaming service specification, and its implementation by a distributed application and can be used for different streaming paradigms, e.g. push and pull services.
Purpose
Although start-ups have gained increasing scholarly attention, we lack sufficient understanding of their entrepreneurial strategic posture (ESP) in emerging economies. The purpose of this study is to examine the processes of ESP of new technology venture start-ups (NTVs) in an emerging market context.
Design/methodology/approach
In line with grounded theory guidelines and the inductive research traditions, the authors adopted a qualitative approach involving 42 in-depth semi-structured interviews with Ghanaian NTV entrepreneurs to gain a comprehensive analysis at the micro-level on the entrepreneurs' strategic posturing. A systematic procedure for data analysis was adopted.
Findings
From the authors' analysis of Ghanaian NTVs, the authors derived a three-stage model to elucidate the nature and process of ESP Phase 1 spotting and exploiting market opportunities, Phase II identifying initial advantages and Phase III ascertaining and responding to change.
Originality/value
The study contributes to advancing research on ESP by explicating the process through which informal ties and networks are utilised by NTVs and NTVs' founders to overcome extreme resource constraints and information vacuums in contexts of institutional voids. The authors depart from past studies in demonstrating how such ties can be harnessed in spotting and exploiting market opportunities by NTVs. On this basis, the paper makes original contributions to ESP theory and practice.
One of the main problematics of the seals tests is the time and money consuming they are. Up to now, there are few tries to do a digitalisation of a test where the seals behaviour can be known.
This work aims to digitally reproduce a seal test to extract their behaviour when working under different operation conditions to see their impact on the pimp’s efficiency. In this thesis, due to the Lomaking effect, the leakage and the forces applied on the stator will be the base of analysis.
First of all, among all the literature available for very different kind of seals and inner patterns, it has been chosen the most appropriate and precise data. The data chosen is “Test results for liquid Damper Seals using a Round-Hole Roughness Pattern for the Stator” from Fayolle, P. and “Static and Rotordynamic Characteristics of Liquid Annular Seals with Circumferentially/Grooved Stator and Smooth Rotor using three levels of circumferential Inlet-Fluid” from Torres J.M.
From the literature, dimensions of the test rig and the seals will be extracted to model them into a 3D CAD software. With the 3D CAD digitalisation, the fluid volumes for a rotor-centred position, meaning without eccentricity, will be extracted, and used. The following components have been modelled:
- Smooth Annular Liquid Seal (Grooved Rotor)
- Grooved Annular Liquid Seal (Smooth Rotor)
- Round-Hole Pattern Annular Liquid Seal (𝐻𝑑=2 𝑚𝑚) (Smooth Rotor)
- Straight Honeycomb Annular Liquid Seal (Smooth Rotor)
- Convergent Honeycomb Annular Liquid Seal (Smooth Rotor)
- Smooth Rotor / Smooth Annular Liquid Seal (Smooth Rotor)
As there is just one test rig, all the components have been adapted to the different dimensions of the seals by referencing some measures. This allows to test any seal with the same test rig.
Afterwards a CFD simulation that will be used to obtain leakage and stator forces. The parameters that will be changed are the rotational velocity of the fluid (2000 rpm, 4000 rpm, and 6000 rpm) and the pressure drop (2,068 bar, 4,137 bar, 6,205 bar, and 8,274 bar).
Those results will be compared to the literature ones, and they will determine if digitalisation can be validated or not. Even though the relative error is higher than 5% but the tendency is the same and it is thought that by changing some parameters the test results can be even closer to the literature ones.
The evolution of cellular networks from its first generation (1G) to its fourth generation (4G) was driven by the demand of user-centric downlink capacity also technically called Mobile Broad-Band (MBB). With its fifth generation (5G), Machine Type Communication (MTC) has been added into the target use cases and the upcoming generation of cellular networks is expected to support them. However, such support requires improvements in the existing technologies in terms of latency, reliability, energy efficiency, data rate, scalability, and capacity.
Originally, MTC was designed for low-bandwidth high-latency applications such as, environmental sensing, smart dustbin, etc. Nowadays there is an additional demand around applications with low-latency requirements. Among other well-known challenges for recent cellular networks such as data rate energy efficiency, reliability etc., latency is also not suitable for mission-critical applications such as real-time control of machines, autonomous driving, tactile Internet etc. Therefore, in the currently deployed cellular networks, there is a necessity to reduce the latency and increase the reliability offered by the networks to support use cases such as, cooperative autonomous driving or factory automation, that are grouped under the denomination Ultra-Reliable Low-Latency Communication (URLLC).
This thesis is primarily concerned with the latency into the Universal Terrestrial Radio Access Network (UTRAN) of cellular networks. The overall work is divided into five parts. The first part presents the state of the art for cellular networks. The second part contains a detailed overview of URLLC use cases and the requirements that must be fulfilled by the cellular networks to support them. The work in this thesis is done as part of a collaboration project between IRIMAS lab in Université de Haute-Alsace, France and Institute for Reliable Embedded Systems and Communication Electronics (ivESK) in Offenburg University of Applied Sciences, Germany. The selected use cases of URLLC are part of the research interests of both partner institutes. The third part presents a detailed study and evaluation of user- and control-plane latency mechanisms in current generation of cellular networks. The evaluation and analysis of these latencies, performed with the open-source ns-3 simulator, were conducted by exploring a broad range of parameters that include among others, traffic models, channel access parameters, realistic propagation models, and a broad set of cellular network protocol stack parameters. These simulations were performed with low-power, low-cost, and wide-range devices, commonly called IoT devices, and standardized for cellular networks. These devices use either LTE-M or Narrowband-IoT (NB-IoT) technologies that are designed for connected things. They differ mainly by the provided bandwidth and other additional characteristics such as coding scheme, device complexity, and so on.
The fourth part of this thesis shows a study, an implementation, and an evaluation of latency reduction techniques that target the different layers of the currently used Long Term Evolution (LTE) network protocol stack. These techniques based on Transmission Time Interval (TTI) reduction and Semi-Persistent Scheduling (SPS) methods are implemented into the ns-3 simulator and are evaluated through realistic simulations performed for a variety of low-latency use cases focused on industry automation and vehicular networking. For testing the proposed latency reduction techniques in cellular networks, since ns-3 does not support NB-IoT in its current release, an NB-IoT extension for LTE module was developed. This makes it possible to explore deployment limitations and issues.
In the last part of this thesis, a flexible deployment framework called Hybrid Scheduling and Flexible TTI for the proposed latency reduction techniques is presented, implemented and evaluated through realistic simulations. With help of the simulation evaluation, it is shown that the improved LTE network proposed and implemented in the simulator can support low-latency applications with low cost, higher range, and narrow bandwidth devices. The work in this thesis points out the potential improvement techniques, their deployment issues and paves the way towards the support for URLLC applications with upcoming cellular networks.
Fifth-generation (5G) cellular mobile networks are expected to support mission-critical low latency applications in addition to mobile broadband services, where fourth-generation (4G) cellular networks are unable to support Ultra-Reliable Low Latency Communication (URLLC). However, it might be interesting to understand which latency requirements can be met with both 4G and 5G networks. In this paper, we discuss (1) the components contributing to the latency of cellular networks and (2) evaluate control-plane and user-plane latencies for current-generation narrowband cellular networks and point out the potential improvements to reduce the latency of these networks, (3) present, implement and evaluate latency reduction techniques for latency-critical applications. The two elements we detected, namely the short transmission time interval and the semi-persistent scheduling are very promising as they allow to shorten the delay to processing received information both into the control and data planes. We then analyze the potential of latency reduction techniques for URLLC applications. To this end, we develop these techniques into the long term evolution (LTE) module of ns-3 simulator and then evaluate the performance of the proposed techniques into two different application fields: industrial automation and intelligent transportation systems. Our detailed evaluation results from simulations indicate that LTE can satisfy the low-latency requirements for a large choice of use cases in each field.
The excessive control signaling in Long Term Evolution networks required for dynamic scheduling impedes the deployment of ultra-reliable low latency applications. Semi-persistent scheduling was originally designed for constant bit-rate voice applications, however, very low control overhead makes it a potential latency reduction technique in Long Term Evolution. In this paper, we investigate resource scheduling in narrowband fourth generation Long Term Evolution networks through Network Simulator (NS3) simulations. The current release of NS3 does not include a semi-persistent scheduler for Long Term Evolution module. Therefore, we developed the semi-persistent scheduling feature in NS3 to evaluate and compare the performance in terms of uplink latency. We evaluate dynamic scheduling and semi-persistent scheduling in order to analyze the impact of resource scheduling methods on up-link latency.