Wiss. Zeitschriftenartikel reviewed: Listung in Master Journal List
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Laser-induced fluorescence (LIF) is a non-invasive optical diagnostics technique frequently used in reactive media to measure physical properties such as gas-phase species concentrations and temperature. It provides important information for understanding reaction and transport processes. For deriving detection schemes that provide selective and quantitative information, fluorescence spectra of the species of interest as well as potential interference sources must be simulated. LIFSim 4.0 is a modular software for simulating absorption, LIF excitation, and LIF emission spectra of NO, SiO, OH, and O2 that also can be extended by the user to include other species. Line positions, line broadening, and collisional quenching are calculated based on spectroscopic data from literature. The code provides spectral analysis tools to interrogate and analyze sensitive spectral regions suitable for derivation of temperature from multi-line LIF measurements. The library includes fitting functions optimized for enhancing and accelerating the post-processing of stacked LIF images with varied excitation wavelength for temperature imaging and separation of the target LIF signal from broad-band or scattering background as well as tools for assessing the validity of results in non-ideal measurement situations.
Given the importance of reducing energy bills in the building sector, especially for schools located in rural areas, where detachment from the grid electricity is recommended, achieving energy self-sufficiency is crucial to provide a conducive indoor environment for students while minimizing energy costs. Therefore, this paper presents a comprehensive methodology aimed at enhancing building energy efficiency, indoor thermal comfort, and achieving net zero energy self-sufficiency for a rural school building, by developing a climate-responsive architectural paradigm for rural schools, ensuring adaptability to diverse environmental conditions while striving for energy independence through passive design strategies. Employing multi-objective optimization with the NSGA-II genetic algorithm, passive design parameters such as construction type, glazing type, insulation specifications, roof vegetation, window overhang, and outdoor shading structures were evaluated across six distinct climatic zones in Morocco. Integration of EnergyPlus, jEPlus, and jEPlus+EA software facilitated the optimization process. Pareto fronts of optimal solutions were generated, prioritizing the minimization of heating and cooling energy consumption alongside discomfort hours. Results demonstrate that the optimized solutions effectively enhance building energy efficiency and indoor thermal comfort while achieving net zero energy status across all studied climatic zones. Optimal solutions enhanced building energy efficiency by 18.6 % - 35.6 %, tailored to climate and school design.
Background/Objectives: The subject of this work is the reconstruction of the inner mechanics of Götz von Berlichingen’s second iron hand. The complex inner mechanics were unknown until Christian von Mechel published a detailed description in 1815. In this artificial hand, each finger can be engaged individually in its three joints and the thumb in one joint. Methods: Based on this description, the individual components were reconstructed at an enlarged scale of 2:1 using computer-aided design (CAD) software and a three-dimensional (3D) printer for the mechanisms. In addition, a finite element method (FEM) analysis was carried out for the components exposed to the greatest stress in order to identify critical areas. Results: By making some adjustments to the mechanics, it was possible to reproduce the mechanisms on a scale of 2:1 on the basis of the index finger. However, when the model was rescaled to 1:1, the internal plastic components were too fragile. This problem was caused by the properties of the 3D printing materials and could be solved by manufacturing the springs from steel. Conclusions: This work aims to make a valuable contribution to the preservation and understanding of the historical artificial second iron hand of Götz von Berlichingen. It once again demonstrates the very precise and detailed craftsmanship of goldsmiths of that time.
Although there do exist a few aeroacoustic studies on harmful artificial phenomena related to the usage of non-uniform Cartesian grids in lattice Boltzmann methods (LBM), a thorough quantitative comparison between different categories of grid arrangement is still missing in the literature. In this paper, several established schemes for hierarchical grid refinement in lattice Boltzmann simulations are analyzed with respect to spurious aeroacoustic emissions using a weakly compressible model based on a D3Q19 athermal velocity set. In order to distinguish between various sources of spurious phenomena, we deploy both the classical Bhatnagar–Gross–Krook and other more recent collision models like the hybrid recursive-regularization operator, the latter of which is able to filter out detrimental non-hydrodynamic mode contributions, inherently present in the LBM dynamics. We show by means of various benchmark simulations that a cell-centered approach, either with a linear or uniform explosion procedure, as well as a vertex-centered direct-coupling method, proves to be the most suitable with regards to aeroacoustics, as they produce the least amount of spurious noise. Furthermore, it is demonstrated how simple modifications in the selection of distribution functions to be reconstructed during the communication step between fine and coarse grids affect spurious aeroacoustic artifacts in vertex-centered schemes and can thus be leveraged to positively influence stability and accuracy.
This contribution introduces the use of convolutional neural networks to detect humans and collaborative robots (cobots) in human–robot collaboration (HRC) workspaces based on their thermal radiation fingerprint. The unique data acquisition includes an infrared camera, two cobots, and up to two persons walking and interacting with the cobots in real industrial settings. The dataset also includes different thermal distortions from other heat sources. In contrast to data from the public environment, this data collection addresses the challenges of indoor manufacturing, such as heat distortions from the environment, and allows for it to be applicable in indoor manufacturing. The Work-Life Robotics Institute HRC (WLRI-HRC) dataset contains 6485 images with over 20 000 instances to detect. In this research, the dataset is evaluated for implementation by different convolutional neural networks: first, one-stage methods, i.e., You Only Look Once (YOLO v5, v8, v9 and v10) in different model sizes and, secondly, two-stage methods with Faster R-CNN with three variants of backbone structures (ResNet18, ResNet50 and VGG16). The results indicate promising results with the best mean average precision at an intersection over union (IoU) of 50 (mAP50) value achieved by YOLOv9s (99.4 %), the best mAP50-95 value achieved by YOLOv9s and YOLOv8m (90.2 %), and the fastest prediction time of 2.2 ms achieved by the YOLOv10n model. Further differences in detection precision and time between the one-stage and multi-stage methods are discussed. Finally, this paper examines the possibility of the Clever Hans phenomenon to verify the validity of the training data and the models’ prediction capabilities.
In this Letter, we calculate the optical and magneto-optical reflectivity in a dielectric/gap/ferromagnet excited by a p-polarized monochromatic optical beam through the prism (Otto configuration) as a function of the angle of incidence θ and the gap thickness d. Besides the well-known surface plasmon polariton (SPP resonance at d ∼ λ), we find a new, to the best of our knowledge, resonance with a nanometric gap d ∼ 10 nm at a large θ ∼ 80°. Both resonances display pronounced resonant behavior in the transverse magneto-optical Kerr effect (T-MOKE).
A deep mutational scanning platform to characterize the fitness landscape of anti-CRISPR proteins
(2024)
Deep mutational scanning is a powerful method for exploring the mutational fitness landscape of proteins. Its adaptation to anti-CRISPR proteins, which are natural CRISPR-Cas inhibitors and key players in the co-evolution of microbes and phages, facilitates their characterization and optimization. Here, we developed a robust anti-CRISPR deep mutational scanning pipeline in Escherichia coli that combines synthetic gene circuits based on CRISPR interference with flow cytometry coupled sequencing and mathematical modeling. Using this pipeline, we characterized comprehensive single point mutation libraries for AcrIIA4 and AcrIIA5, two potent inhibitors of CRISPR-Cas9. The resulting mutational fitness landscapes revealed considerable mutational tolerance for both Acrs, suggesting an intrinsic redundancy with respect to Cas9 inhibitory features, and – for AcrIIA5 – indicated mutations that boost Cas9 inhibition. Subsequent in vitro characterization suggested that the observed differences in inhibitory potency between mutant inhibitors were mostly due to changes in binding affinity rather than protein expression levels. Finally, to demonstrate that our pipeline can inform Acrs-based genome editing applications, we employed a selected subset of mutant inhibitors to increase CRISPR-Cas9 target specificity by modulating Cas9 activity. Taken together, our work establishes deep mutational scanning as a powerful method for anti-CRISPR protein characterization and optimization.
Anisotropic attenuation of GHz-frequency acoustic phonons and the Grüneisen tensor in MgO crystal
(2024)
We report on measurements of the anisotropy of velocities and attenuation of GHz-frequency acoustic phonons in a cubic MgO crystal at room temperature. They are used to quantify the strong anisotropy of the Grüneisen parameter and calculate the attenuation anisotropy for Rayleigh surface acoustic waves. These observations constitute important building blocks for better understanding of ultrafast laser-based magneto-acoustic and nonlinear acoustic experiments at ultrahigh frequencies.
Dieser Beitrag beleuchtet die wichtige Rolle, die Strategieentwicklung im Allgemeinen und im Speziellen für Industriebetriebe spielt. Zunächst wird die Historie der Strategieentwicklung aufgezeigt und die Herausforderungen, denen insbesondere Industriebetriebe gegenüberstehen, erläutert. Im Anschluss wird erörtert, wie eine Strategieentwicklung aufgebaut ist und methodisch sauber durchgeführt wird. Abschließend fasst der Beitrag die wichtigsten Schritte in Form einer strukturierten Grafik zusammen und erläutert die Bedeutung der Strategieentwicklung für Industriebetriebe.
Quantifying movement coordination in cross-country (XC) skiing, specifically the technique with its elemental forms, is challenging. Particularly, this applies when trying to establish a bidirectional transfer between scientific theory and practical experts' knowledge as expressed, for example, in ski instruction curricula. The objective of this study was to translate 14 curricula-informed distinct elements of the V2 ski-skating technique (horizontal and vertical posture, lateral tilt, head position, upper body rotation, arm swing, shoulder abduction, elbow flexion, hand and leg distance, plantar flexion, ski set-down, leg push-off, and gliding phase) into plausible, valid and applicable measures to make the technique training process more quantifiable and scientifically grounded. Inertial measurement unit (IMU) data of 10 highly experienced XC skiers who demonstrated the technique elements by two extreme forms each (e.g., anterior versus posterior positioning for the horizontal posture) were recorded. Element-specific principal component analyses (PCAs)—driven by the variance produced by the technique extremes—resulted in movement components that express quantifiable measures of the underlying technique elements. Ten measures were found to be sensitive in distinguishing between the inputted extreme variations using statistical parametric mapping (SPM), whereas for four elements the SPM did not detect differences (lateral tilt, plantar flexion, ski set-down, and leg push-off). Applicability of the established technique measures was determined based on quantifying individual techniques through them. The study introduces a novel approach to quantitatively assess V2 ski-skating technique, which might help to enhance technique feedback and bridge the communication gap that often exists between practitioners and scientists.