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Uncontrollable manufacturing variations in electrical hardware circuits can be exploited as Physical Unclonable Functions (PUFs). Herein, we present a Printed Electronics (PE)-based PUF system architecture. Our proposed Differential Circuit PUF (DiffC-PUF) is a hybrid system, combining silicon-based and PE-based electronic circuits. The novel approach of the DiffC-PUF architecture is to provide a specially designed real hardware system architecture, that enables the automatic readout of interchangeable printed DiffC-PUF core circuits. The silicon-based addressing and evaluation circuit supplies and controls the printed PUF core and ensures seamless integration into silicon-based smart systems. Major objectives of our work are interconnected applications for the Internet of Things (IoT).