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BACKGROUND
Various neutral and alkaline peptidases are commercially available for use in protein hydrolysis under neutral to alkaline conditions. However, the hydrolysis of proteins under acidic conditions by applying fungal aspartic peptidases (FAPs) has not been investigated in depth so far. The aim of this study, thus, was to purify a FAP from the commercial enzyme preparation, ROHALASE® BXL, determine its biochemical characteristics, and investigate its application for the hydrolysis of food and animal feed proteins under acidic conditions.
RESULTS
A Trichoderma reesei derived FAP, with an apparent molecular mass of 45.8 kDa (sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SDS-PAGE) was purified 13.8-fold with a yield of 37% from ROHALASE® BXL. The FAP was identified as an aspartate protease (UniProt ID: G0R8T0) by inhibition and nano-LC-ESI-MS/MS studies. The FAP showed the highest activity at 50°C and pH 4.0. Monovalent cations, organic solvents, and reducing agents were tolerated well by the FAP. The FAP underwent an apparent competitive product inhibition by soy protein hydrolysate and whey protein hydrolysate with apparent Ki-values of 1.75 and 30.2 mg*mL−1, respectively. The FAP showed promising results in food (soy protein isolate and whey protein isolate) and animal feed protein hydrolyses. For the latter, an increase in the soluble protein content of 109% was noted after 30 min.
CONCLUSION
Our results demonstrate the applicability of fungal aspartic endopeptidases in the food and animal feed industry. Efficient protein hydrolysis of industrially relevant substrates such as acidic whey or animal feed proteins could be conducted by applying fungal aspartic peptidases. © 2022 Society of Chemical Industry.
This review provides an overview on the production and analysis techniques of antioxidative peptides from food proteins. Regarding the production of antioxidative peptides, interlinked factors must be considered. Depending on the protein substrate, different peptidases or peptidase systems containing multiple enzymes as well as a specific production process must be chosen. The antioxidative peptides might be produced in a batch process including multiple pre- and post-treatments, besides the hydrolyses with peptidases itself. As an alternative, the potential of continuous production systems is discussed in this review. Furthermore, robust analyses tools are needed to gain control of the process and final product properties. With no standardized methodology available for antioxidative peptide evaluation, pros and cons of various strategies for peptide separation and antioxidative measurement are discussed in this review. Therefore, this review provides a roadmap for antioxidative peptide generation from various sources for research and development as well as for potential industrial use.
Gas Analysis and Optimization of Debinding and Sintering Processes for Metallic Binder-Based AM*
(2022)
Binder-based additive manufacturing processes for metallic
AM components in a wide range of applications usually use
organic binders and process-related additives that must be
thermally removed before sintering. Debinding processes are
typically parameterized empirically and thus far from the optimum.
Since debinding based on thermal decomposition processes
of organic components and the subsequent thermochemical
reactions between process atmosphere and metal
powder materials make uncomplicated parameterization difficult,
in-situ instrumentation was introduced at Fraunhofer
IFAM. This measurement method relies on infrared spectroscopy
and mass spectrometry in various furnace concepts to
understand the gas processes of decomposition of organic
components and the subsequent thermochemical reactions
between the carrier gas atmosphere and the metal part, as well
as their kinetics. This method enables an efficient optimization
of the temperature-time profiles and the required atmosphere
composition to realize dense AM components with low contamination.
In the paper, the optimization strategy is presented,
and the achievable properties are illustrated using a fused
filament fabrication (FFF) component example made of 316L
stainless steel.
In the literature, many studies have described the 3D printing of ceramic-based scaffolds (e.g., printing with calcium phosphate cement) in the form of linear structures with layer rotations of 90°, although no right angles can be found in the human body. Therefore, this work focuses on the adaptation of biological shapes, including a layer rotation of only 1°. Sample shapes were printed with calcium phosphate cement using a 3D Bioplotter from EnvisionTec. Both straight and wavy spokes were printed in a round structure with 12 layers. Depending on the strand diameter (200 and 250 µm needle inner diameter) and strand arrangement, maximum failure loads of 444.86 ± 169.39 N for samples without subsequent setting in PBS up to 1280.88 ± 538.66 N after setting in PBS could be achieved.
Die Optimierung der Auftragsterminierung und Einsteuerungsreihenfolge hat großen Einfluss auf die Produktivität von Fertigungssystemen. Genetische Algorithmen und Simulation sind verbreitete Werkzeuge zur Optimierung. Dieser Beitrag beschreibt einen neuen Ansatz zur Optimierung durch einen genetischen Algorithmus und der Simulation in dynamischen Modellen. Eine illustrative Fallstudie validiert den Ansatz und zeigt das Potenzial zur ganzheitlichen Verbesserung von Fertigungssystemen auf.
Titanium and stainless steel are commonly known as osteosynthesis materials with high strength and good biocompatibility. However, they have the big disadvantage that a second operation for hardware removal is necessary. Although resorbable systems made of polymers or magnesium are increasingly used, they show some severe adverse foreign body reactions or unsatisfying degradation behavior. Therefore, we started to investigate molybdenum as a potential new biodegradable material for osteosynthesis in craniomaxillofacial surgery. To characterize molybdenum as a biocompatible material, we performed in vitro assays in accordance with ISO Norm 10993-5. In four different experimental setups, we showed that pure molybdenum and molybdenum rhenium alloys do not lead to cytotoxicity in human and mouse fibroblasts. We also examined the degradation behavior of molybdenum by carrying out long-term immersion tests (up to 6 months) with molybdenum sheet metal. We showed that molybdenum has sufficient mechanical stability over at least 6 months for implants on the one hand and is subject to very uniform degradation on the other. The results of our experiments are very promising for the development of new resorbable osteosynthesis materials for craniomaxillofacial surgery based on molybdenum.
Bislang gibt es keine Güterstraßenbahnsysteme, die im urbanen Warentransport im Realbetrieb eingesetzt werden. Bestehende Konzepte sind auf einzelne Branchen, ausgewählte Transportgüter oder einzelne Verlader ausgerichtet. Untersuchungen zu Güterstraßenbahnprojekten konzentrieren sich auf individuelle Kunden (zum Beispiel "CarGo Tram" Dresden). Für die Realisierung einer Güterstraßenbahn im urbanen Raum wäre zu klären, welche Anforderungen potenzielle Nutzer haben und wie diese Anforderungen in ein logistisches Konzept integriert werden können. In einer multiplen Fallstudie werden drei Unternehmen aus verschiedenen Branchen analysiert. Aufgrund heterogener Anforderungen wird ein modulares Logistikkonzept vorgeschlagen. Der Beitrag entstand im Rahmen des Projektes "LogIKTram: Logistikkonzept und IKT-Plattform für stadtbahnbasierten Gütertransport".
Acoustic waves are investigated which are guided at the edge (apex line) of a wedge-shaped elastic body or at the edge of an elastic plate. The edges contain a periodic sequence of modifications, consisting either of indentations or inclusions with a different elastic material which gives rise to high acoustic mismatch. Dispersion relations are computed with the help of the finite element method. They exhibit zero-group velocity points on the dispersion branches of edge-localized acoustic modes. These special points also occur at Bloch-Floquet wavenumbers away from the Brillouin zone boundary. Deep indentations lead to flat branches corresponding to largely non-interacting, Einstein-oscillator like vibrations of the tongues between the grooves of the periodic structure. Due to the nonlinearity of the elastic media, quantified by their third-order elastic constants, an acoustic mode localized at a periodically modified edge generates a second harmonic which partly consists of surface and plate modes propagating into the elastic medium in the direction vertical to the edge. This acoustic radiation at the second-harmonic frequency is investigated for an elastic plate and a truncated sharp-angle wedge with periodic inclusions at their edges. Unlike nonlinear bulk wave generation by surface acoustic waves in an interdigital structure, surface and plate mode radiation by edge-localized modes can be visualized directly in laser-ultrasound experiments.