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In recent years, physically unclonable functions (PUFs) have gained significant attraction in IoT security applications, such as cryptographic key generation and entity authentication. PUFs extract the uncontrollable production characteristics of different devices to generate unique fingerprints for security applications. When generating PUF-based secret keys, the reliability and entropy of the keys are vital factors. This study proposes a novel method for generating PUF-based keys from a set of measurements. Firstly, it formulates the group-based key generation problem as an optimization problem and solves it using integer linear programming (ILP), which guarantees finding the optimum solution. Then, a novel scheme for the extraction of keys from groups is proposed, which we call positioning syndrome coding (PSC). The use of ILP as well as the introduction of PSC facilitates the generation of high-entropy keys with low error correction costs. These new methods have been tested by applying them on the output of a capacitor network PUF. The results confirm the application of ILP and PSC in generating high-quality keys.
Physical unclonable functions (PUFs) are increasingly generating attention in the field of hardware-based security for the Internet of Things (IoT). A PUF, as its name implies, is a physical element with a special and unique inherent characteristic and can act as the security anchor for authentication and cryptographic applications. Keeping in mind that the PUF outputs are prone to change in the presence of noise and environmental variations, it is critical to derive reliable keys from the PUF and to use the maximum entropy at the same time. In this work, the PUF output positioning (POP) method is proposed, which is a novel method for grouping the PUF outputs in order to maximize the extracted entropy. To achieve this, an offset data is introduced as helper data, which is used to relax the constraints considered for the grouping of PUF outputs, and deriving more entropy, while reducing the secret key error bits. To implement the method, the key enrollment and key generation algorithms are presented. Based on a theoretical analysis of the achieved entropy, it is proven that POP can maximize the achieved entropy, while respecting the constraints induced to guarantee the reliability of the secret key. Moreover, a detailed security analysis is presented, which shows the resilience of the method against cyber-security attacks. The findings of this work are evaluated by applying the method on a hybrid printed PUF, where it can be practically shown that the proposed method outperforms other existing group-based PUF key generation methods.
This paper will introduce the open-source model MyPyPSA-Ger, a myopic optimization model developed to represent the German energy system with a detailed mapping of the electricity sector, on a highly disaggregated level, spatially and temporally, with regional differences and investment limitations. Furthermore, this paper will give new outlooks on the German federal government 2050 emissions goals of the electricity sector to become greenhouse gas neutral by proposing new CO2 allowance strategies. Moreover, the regional differences in Germany will be discussed, their role and impact on the energy transition, and which regions and states will drive the renewable energy utilization forward.
Following a scenario-based analysis, the results point out the major keystones of the energy transition path from 2020 to 2050. Solar, onshore wind, and gas-fired power plants will play a fundamental role in the future electricity systems. Biomass, run of river, and offshore wind technologies will be utilized in the system as base-load generation technologies. Solar and onshore wind will be installed almost everywhere in Germany. However, due to the nature of Germany’s weather and geographical features, the southern and northern regions will play a more important role in the energy transition.
Higher CO2 allowance costs will help achieve the 1.5-degree-target of the electricity system and will allow for a rapid transition. Moreover, the more expensive, and the earlier the CO2 tax is applied to the system, the less it will cost for the energy transition, and the more emissions will be saved throughout the transition period. An earlier phase-out of coal power plants is not necessary with high CO2 taxes, due to the change in power plant’s unit commitment, as they prioritize gas before coal power plants. Having moderate to low CO2 allowance cost or no clear transition policy will be more expensive and the CO2 budget will be exceeded. Nonetheless, even with no policy, renewables still dominate the energy mix of the future.
However, maintaining the maximum historical installation rates of both national and regional levels, with the current emissions reduction strategy, will not be enough to reach the level of climate-neutral electricity system. Therefore, national and regional installation requirements to achieve the federal government emission reduction goals are determined. Energy strategies and decision makers will have to resolve great challenges in order to stay in line with the 1.5-degree-target.
BACKGROUND
Various neutral and alkaline peptidases are commercially available for use in protein hydrolysis under neutral to alkaline conditions. However, the hydrolysis of proteins under acidic conditions by applying fungal aspartic peptidases (FAPs) has not been investigated in depth so far. The aim of this study, thus, was to purify a FAP from the commercial enzyme preparation, ROHALASE® BXL, determine its biochemical characteristics, and investigate its application for the hydrolysis of food and animal feed proteins under acidic conditions.
RESULTS
A Trichoderma reesei derived FAP, with an apparent molecular mass of 45.8 kDa (sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SDS-PAGE) was purified 13.8-fold with a yield of 37% from ROHALASE® BXL. The FAP was identified as an aspartate protease (UniProt ID: G0R8T0) by inhibition and nano-LC-ESI-MS/MS studies. The FAP showed the highest activity at 50°C and pH 4.0. Monovalent cations, organic solvents, and reducing agents were tolerated well by the FAP. The FAP underwent an apparent competitive product inhibition by soy protein hydrolysate and whey protein hydrolysate with apparent Ki-values of 1.75 and 30.2 mg*mL−1, respectively. The FAP showed promising results in food (soy protein isolate and whey protein isolate) and animal feed protein hydrolyses. For the latter, an increase in the soluble protein content of 109% was noted after 30 min.
CONCLUSION
Our results demonstrate the applicability of fungal aspartic endopeptidases in the food and animal feed industry. Efficient protein hydrolysis of industrially relevant substrates such as acidic whey or animal feed proteins could be conducted by applying fungal aspartic peptidases. © 2022 Society of Chemical Industry.
A novel peptidyl-lys metalloendopeptidase (Tc-LysN) from Tramates coccinea was recombinantly expressed in Komagataella phaffii using the native pro-protein sequence. The peptidase was secreted into the culture broth as zymogen (~38 kDa) and mature enzyme (~19.8 kDa) simultaneously. The mature Tc-LysN was purified to homogeneity with a single step anion-exchange chromatography at pH 7.2. N-terminal sequencing using TMTpro Zero and mass spectrometry of the mature Tc-LysN indicated that the pro-peptide was cleaved between the amino acid positions 184 and 185 at the Kex2 cleavage site present in the native pro-protein sequence. The pH optimum of Tc-LysN was determined to be 5.0 while it maintained ≥60% activity between pH values 4.5—7.5 and ≥30% activity between pH values 8.5—10.0, indicating its broad applicability. The temperature maximum of Tc-LysN was determined to be 60 °C. After 18 h of incubation at 80 °C, Tc-LysN still retained ~20% activity. Organic solvents such as methanol and acetonitrile, at concentrations as high as 40% (v/v), were found to enhance Tc-LysN’s activity up to ~100% and ~50%, respectively. Tc-LysN’s thermostability, ability to withstand up to 8 M urea, tolerance to high concentrations of organic solvents, and an acidic pH optimum make it a viable candidate to be employed in proteomics workflows in which alkaline conditions might pose a challenge. The nano-LC-MS/MS analysis revealed bovine serum albumin (BSA)’s sequence coverage of 84% using Tc-LysN which was comparable to the sequence coverage of 90% by trypsin peptides.
A systematic toxicological analysis procedure using high-performance thin layer chromatography in combination with fibre optical scanning densitometry for identification of drugs in biological samples is presented. Two examples illustrate the practicability of the technique. First, the identification of a multiple intake of analgesics: codeine, propyphenazone, tramadol, flupirtine and lidocaine, and second, the detection of the sedative diphenhydramine. In both cases, authentic urine specimens were used. The identifications were carried out by an automatic measurement and computer-based comparison of in situ UV spectra with data from a compiled library of reference spectra using the cross-correlation function. The technique allowed a parallel recording of chromatograms and in situ UV spectra in the range of 197–612 nm. Unlike the conventional densitometry, a dependency of UV spectra by concentration of substance in a range of 250–1000 ng/spot was not observed.
Blockchain-IIoT integration into industrial processes promises greater security, transparency, and traceability. However, this advancement faces significant storage and scalability issues with existing blockchain technologies. Each peer in the blockchain network maintains a full copy of the ledger which is updated through consensus. This full replication approach places a burden on the storage space of the peers and would quickly outstrip the storage capacity of resource-constrained IIoT devices. Various solutions utilizing compression, summarization or different storage schemes have been proposed in literature. The use of cloud resources for blockchain storage has been extensively studied in recent years. Nonetheless, block selection remains a substantial challenge associated with cloud resources and blockchain integration. This paper proposes a deep reinforcement learning (DRL) approach as an alternative to solving the block selection problem, which involves identifying the blocks to be transferred to the cloud. We propose a DRL approach to solve our problem by converting the multi-objective optimization of block selection into a Markov decision process (MDP). We design a simulated blockchain environment for training and testing our proposed DRL approach. We utilize two DRL algorithms, Advantage Actor-Critic (A2C), and Proximal Policy Optimization (PPO) to solve the block selection problem and analyze their performance gains. PPO and A2C achieve 47.8% and 42.9% storage reduction on the blockchain peer compared to the full replication approach of conventional blockchain systems. The slowest DRL algorithm, A2C, achieves a run-time 7.2 times shorter than the benchmark evolutionary algorithms used in earlier works, which validates the gains introduced by the DRL algorithms. The simulation results further show that our DRL algorithms provide an adaptive and dynamic solution to the time-sensitive blockchain-IIoT environment.
An Overview of Technologies for Improving Storage Efficiency in Blockchain-Based IIoT Applications
(2022)
Since the inception of blockchain-based cryptocurrencies, researchers have been fascinated with the idea of integrating blockchain technology into other fields, such as health and manufacturing. Despite the benefits of blockchain, which include immutability, transparency, and traceability, certain issues that limit its integration with IIoT still linger. One of these prominent problems is the storage inefficiency of the blockchain. Due to the append-only nature of the blockchain, the growth of the blockchain ledger inevitably leads to high storage requirements for blockchain peers. This poses a challenge for its integration with the IIoT, where high volumes of data are generated at a relatively faster rate than in applications such as financial systems. Therefore, there is a need for blockchain architectures that deal effectively with the rapid growth of the blockchain ledger. This paper discusses the problem of storage inefficiency in existing blockchain systems, how this affects their scalability, and the challenges that this poses to their integration with IIoT. This paper explores existing solutions for improving the storage efficiency of blockchain–IIoT systems, classifying these proposed solutions according to their approaches and providing insight into their effectiveness through a detailed comparative analysis and examination of their long-term sustainability. Potential directions for future research on the enhancement of storage efficiency in blockchain–IIoT systems are also discussed.
Passive solar elements for both direct and indirect gains, are systems used to maintain a comfortable living environment while saving energy, especially in the building energy retrofit and adaptation process. Sunspaces, thermal mass and glazing area and orientation have been often used in the past to guarantee adequate indoor conditions when mechanical devices were not available. After a period of neglect, nowadays they are again considered as appropriate systems to help face environmental issues in the building sector, and both international and national legislation takes into consideration the possibility of including them in the building planning tools, also providing economic incentives. Their proper design needs dynamic simulation, often difficult to perform and time consuming. Moreover, results generally suffer from several uncertainties, so quasi steady-state procedures are often used in everyday practice with good results, but some corrections are still needed. In this paper, a comparative analysis of different solutions for the construction of verandas in an existing building is presented, following the procedure provided by the slightly modified and improved Standard EN ISO 13790:2008. Advantages and disadvantages of different configurations considering thermal insulation, windows typology and mechanical ventilation systems are discussed and a general intervention strategy is proposed. The aim is to highlight the possibility of using sunspaces in order to increase the efficiency of the existing building stock, considering ease of construction and economic viability.
Energy Performance of Verandas in the Building Retrofit Process (PDF Download Available). Available from: https://www.researchgate.net/publication/303093420_Energy_Performance_of_Verandas_in_the_Building_Retrofit_Process [accessed Jul 5, 2017].