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We present a two-dimensional (2D) planar chromatographic separation method for phytoestrogenic active compounds on RP-18 W (Merck, 1.14296) phase. It could be shown that an ethanolic extract of liquorice (Glycyrrhiza glabra) roots contains four phytoestrogenic active compounds. As solvent, in the first direction, the mix of hexane, ethyl acetate, and acetone (45:15:10, v/v) was used, and, in the second direction, that of acetone and water (15:10, v/v) was used. After separation, a modified yeast estrogen screen (YES) test was applied, using the yeast strain Saccharomyces cerevisiae BJ3505. The test strain (according to McDonnell) contains the estrogen receptor. Its activation by estrogen active compounds is measured by inducing the reporter gene lacZ which encodes the enzyme β-galactosidase. This enzyme activity is determined on plate by using the fluorescent substrate MUG (4-methylumbelliferyl-β-d-galactopyranoside). The enzyme can also hydrolyse X-β-Gal (5-bromo-4-chloro-3-indoxyl-β-d-galactopyranosid) into β-galactose and 5-bromo-4-chloro-3-indoxyl. The indoxyl compound is oxidized by oxygen forming the deep-blue dye 5,5β-dibromo-4,4β-dichloro-indigo which allows to detect phytoestrogenic activity more specific in the presence of native fluorescing compounds.
Viele hochbeanspruchte Bauteile müssen zur Erfüllung ihres konstruktiven Zwecks mit Durchdringungskerben versehen werden. Infolge der gegenseitigen Wechselwirkung gelten für die Kerbwirkung dieser Art von Mehrfachkerben andere Gesetzmäßigkeiten als bei Einzelkerben. Die Weiterentwicklung der Lehre von der Tragfähigkeitsberechnung höchstbeanspruchter Maschinenelemente macht es notwendig, sich mit der Durchdringungskerbwirkung eingehend zu befassen. Thum und Svenson [1] entwickelten im Jahr 1949 ein Näherungsverfahren zur Abschätzung der Formzahl an einem zugbelasteten Stab mit Durchdringungskerben. In vielen Lehrbüchern findet dieses Verfahren Anwendung. Aus heutiger Sicht erscheint die Eignung der aus diesem Ansatz erzielten Ergebnisse als dringend überprüfungswürdig. Das thum’sche Verfahren wird unter die Lupe genommen. Der hier vorliegende Beitrag präsentiert mit Hilfe der Finiten-Elemente-Methode (FEM) neue Untersuchungsergebnisse an zugbeanspruchten Stäben mit Halbkreisnut und überlagerter Querbohrung. Diese ergaben, dass die Berechnung nach [1] Lücken aufweist. Ihr Ansatz stellt für den heutigen Entwicklungsstand eine mit zu großen Abweichungen behaftete Näherungshypothese dar.
Three real-lab trigeneration microgrids are investigated in non-residential environments (educational, office/administrational, companies/production) with a special focus on domain-specific load characteristics. For accurate load forecasting on such a local level, à priori information on scheduled events have been combined with statistical insight from historical load data (capturing information on not explicitly-known consumer behavior). The load forecasts are then used as data input for (predictive) energy management systems that are implemented in the trigeneration microgrids. In real-world applications, these energy management systems must especially be able to carry out a number of safety and maintenance operations on components such as the battery (e.g. gassing) or CHP unit (e.g. regular test runs). Therefore, energy management systems should combine heuristics with advanced predictive optimization methods. Reducing the effort in IT infrastructure the main and safety relevant management process steps are done on site using a Smart & Local Energy Controller (SLEC) assisted by locally measured signals or operator given information as default and external inputs for any advanced optimization. Heuristic aspects for local fine adjustment of energy flows are presented.
A former remote area power supply was converted to a smart cogeneration subnet with combined heat and power to develop and validate a forecast based energy management at the University of Applied Sciences in Offenburg/Germany. Locally processed weather forecasts and forecasted demand profiles are integrated to allow a precise reaction to changes of fluctuating power sources, changes in scheduled demand profiles and to improve the energy efficiency of the supply. The management of the electrical and thermal storages is influenced by the forecasted energy contributions and the forecasted demand. Further approaches should improve the accuracy of forecasting algorithms and integrate parameter models gained of a detailed monitoring to realize predictive controllers.
Polygeneration systems are a key technology for the reduction of primary energy usage and emissions. High costs, lack of flexibility and effort for parameterization hinder the wide usage of modeling tools during their conceptual design. This paper describes how planning tools can be structured for the conceptual design phase where only little information is available to the planner. A library concept was developed using the principles of object-oriented modeling to address the flexibility issue. With respect to cost and expandability, the open-source modeling language Modelica was chosen. Furthermore, easy-to-parameterize component models were developed. In addition to the improved library concept and novel component models, an easy-to-adapt control concept is proposed. The component models were validated and the applicability of the library was demonstrated by means of an example. It was shown that the data usually obtained from spec sheets are sufficient to parameterize the models. In addition to this, the control concept was approved.
Micro gas turbines (MGTs) are regarded as combined heat and power (CHP) units which offer high fuel utilization and low emissions. They are applied in decentralized energy neration.
To facilitate the planning process of energy systems, namely in the context of the increasing application of optimization techniques, there is a need for easy-to-parametrize component models with sufficient accuracy which allow a fast computation. In this paper, a model is proposed where the non-linear part load characteristics of the MGT are linearized by means of physical insight of the working principles of turbomachinery. Further, it is shown that the model can be parametrized by the data usually available in spec sheets. With this model a uniform description of MGTs from several manufacturers
covering an electrical power range from 30kW to 333kW can be obtained. The MGT model was
implemented by means of Modelica/Dymola. The resulting MGT system model, comprising further heat exchangers and hydraulic components, was validated using the experimental data of a 65kW MGT from a trigeneration energy system.