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Self-sufficient enzymes belong to the cytochrome P450 (CYP) group and are known for their superior hydroxylation catalytic activity. In the pursuit of identifying new pesticides to combat antimicrobial-resistant pathogens, we employed BM3 wild type (BM3-WT), the fastest monohydroxylating CYP, along with its seven homologs, to investigate the production of potential hydroxylated derivatives from the established pesticide, 4-oxocrotonic acid using high-pressure liquid chromatography (HPLC) method. Following the recombinant production of BM3-WT and three other homologs in E. coli, and their subsequent purification using Immobilized Metal Affinity Chromatography (IMAC), a novel enzyme assay approach was developed as a substitute for the carbon monoxide (CO) assay. This new method relied on the measurement of NADPH consumption at 340 nm by BM3-WT for palmitic acid. Leveraging this established technique, we explored the substrate specificity of BM3-WT and its homologs not only on palmitic acid but also on other structurally similar compounds, including 4-oxocrotonic acid. The results obtained from the established NADPH assay indicate that all tested enzymes displayed greater catalytic activity on 4-oxocrotonic acid in comparison to other substrates with similar structures. However, the impact of BM3-WT and its homologs on 4-oxocrotonic acid varied in terms of product specificity. Enzymes such as Poh, Trr and Bas-CYP D exhibited specificity in producing solely monohydroxylated products, while others tended to yield dehydroxylated and ketol metabolites.
In recent years, the demand for reliable power, driven by sensitive electronic equipment, has surged. Even minor deviations from the nominal supply can lead to malfunctions or failure. Despite technological advancements, power quality issues persist due to various factors like short circuits, overloads, voltage fluctuations, unbalanced loads, and non-linear loads.
This thesis extensively explores power quality anomalies in industrial and commercial sectors, using power system data as the primary analytical resource. It addresses the critical need for power supply reliability in today's evolving power grid industry, affected by non-linear loads, renewable energy integration, and electric vehicles. This field of study is paramount for ensuring power supply reliability and stability in the evolving power grid industry.
The core of this thesis involves a comprehensive investigation of power quality, with a focus on frequency, power, and harmonics in voltage and current signals. The research employs Python programming for advanced data analysis, utilizing techniques such as advanced Fast Fourier Transformation (FFT) analysis. The primary objective is to provide valuable insights aimed at elevating power supply quality and enhancing reliability in both industrial and commercial environments.
The purpose of this master's thesis was to set up a test bed for the absorption of chemical compounds by carbon-based sorbents and polymers and to develop a method for the detection of these substances applied by liquid chromatography.
The study made it possible to demonstrate the effectiveness of both polymers and biochars sorbents for the adosorption of specific substances. The results obtained open new paths on the study of biochar for the treatment of contaminated water. Some biochars made from plant-based materials have been shown to be almost as effective as commercial products used in plants. The developed chromatography method allows efficient separation of substances and their detection.
Estimation and projecting total steel industry production costs from 2019 to 2030 for Germany
(2023)
This thesis analyses the total production cost of the German steel industry from 2019 to 2022, as well as a projection of the German steel industry's total production cost until 2030. The research separates the costs of steel production into their primary components, such as raw materials, energy, CO2 cost, capital expenses and operating expenses. The cost of steel production is determined separately for primary steelmaking with the blast furnace and basic oxygen furnace (BF-BOF) and secondary steelmaking with the electric arc furnace (EAF).
The analysis indicates that, following the COVID-19 disaster and the fuel crisis, the overall cost of producing steel in Germany has progressively risen over the previous few years, reaching its peak in the first half of 2022. In addition, there are considerable disparities between the production costs of primary and secondary steelmaking processes, with primary steelmaking generally being more expensive.
In this analysis, the total cost of production for the German steel industry in the year 2030 has been estimated by taking into account historical trends as well as other predictions that are currently available.
This thesis provides overall insights on the economics of the German steel sector. By giving thorough information on production costs and changes over time, this research can assist guide crucial future investment decisions in this essential industry. To ensure long-term success, our findings emphasize the significance of investing in more sustainable and ecologically friendly steel production processes.
As the population grows, so does the amount of biowaste. As demand for energy grows, biogas is a promising solution to the problem. Lignocellulosic materials are challenged of slow degradability due to the presence of polymers such as cellulose, lignin and hemicellulose. There are several pretreatment methods available to enhance the degradability of such materials, including enzymatic pretreatment. In this pretreatment, there are few parameters that can influence the results, the most important being the enzyme to solid ratio and the solid to liquid ratio. During this project, experiments were conducted to determine the optimal conditions for those two factors. It was discovered that a solid to liquid ratio of 31 g of buffer per 1 gram of organic dry matter produced the highest reducing sugar release in flasks when combined with 34 mg of protein per 1 gram of organic dry mass. Additionally, another experiment was carried out to investigate the impact of enzymatic pretreatment on biogas production using artificial biowaste as a substrate. Artificial biowaste produced 577,9 NL/kg oDM, while enzymatically pretreated biowaste produced 639,3 NL/kg oDM. This resulted in a 10,6% rise in cumulative biogas production compared to its use without enzymatic pretreatment. By the conclusion of the investigation, specific cumulative dry methane yields of 364,7 NL/kg oDM and 426,3 NL/kg oDM were obtained from artificial biowaste without and with enzymatic pretreatment, respectively. This resulted in a methane production boost of 16,9%. Additionally in case of the reactors with enzymatically pretreated substrate kinetic constant was lower more than double, where maximum volume of biogas increased, comparing to the reactors without enzymatic pretreatment.
The cellulase-producing Trichoderma reesei strain RL-P37 exhibits significant potential, yielding 7.3 g/L of cellulase in 241 hours. Microscopic investigations reveal a link between spore formation and enzyme production, suggesting the need for research into the intricate relationship between enzyme production, stress responses, and the nutritional prerequisites of fungi. Comparatively, the use of sodium hydroxide (NaOH) treatment, as opposed to water treatment, results in the reduction of micronutrient content and carbon source extraction as filtrate. Despite these challenges, research by He et al. (2021) highlights NaOH's efficiency in cellulose extraction from plant-based sources. Using NaOH pretreatment can be proven as effective by designing a proper cultivation method. The selection of inducers for enzyme induction gains importance, with soluble inducers, as emphasized by Zhang et al. (2022), exhibiting superior effectiveness. Hence, adopting soluble inducers in designing cultivation methods for improved enzyme production in shaking flasks is recommended. Enzymatic treatment of bio-waste, as outlined by Hu et al. (2021), shows promise in augmenting essential component content by breaking down plant cell walls and intercellular compartments. However, the feasibility of using an artificial bio-waste medium for cultivating Trichoderma reesei is questioned. Investigating the impact of micronutrient levels, particularly the inhibitory role of zinc, on fungal growth becomes essential. These findings underscore the necessity for ongoing research and optimization in cellulase production, emphasizing both strain productivity and cultivation methodologies.
Decarbonisation Strategies in Energy Systems Modelling: APV and e-tractors as Flexibility Assets
(2023)
This work presents an analysis of the impact of introducing Agrophotovoltaic technologies and electric tractors into Germany’s energy system. Agrophotovoltaics involves installing photovoltaic systems in agricultural areas, allowing for dual usage of the land for both energy generation and food production. Electric tractors, which are agricultural machinery powered by electric motors, can also function as energy storage units, providing flexibility to the grid. The analysis includes a sensitivity study to understand how the availability of agricultural land influences Agrophotovoltaic investments, followed by the examination of various scenarios that involve converting diesel tractors to electric tractors. These scenarios are based on the current CO2 emission reduction targets set by the German Government, aiming for a 65% reduction below 1990 levels by 2030 and achieving zero emissions by 2045. The results indicate that approximately 3% of available agricultural land is necessary to establish a viable energy mix in Germany. Furthermore, the expansion of electric tractors tends to reduce the overall system costs and enhances the energy-cost-efficiency of Agrophotovoltaic investments.
The interest of scientists to study motion sequences exists in the fields of sports science, clinical analysis and computer animation for quite some time. While in the last decades mainly markerbased motion capture systems have been used to evaluate movements, the interest in markerless systems is growing more and more. Nevertheless, in the field of clinical analysis, markerless methods have not yet proven their value, partly due to a lack of studies evaluating the quality of the obtained data. Therefore, this study aims to validate two markerless motion capture softwares from Simi Reality Motion Systems. The software Simi Shape, which is a mixture of traditional image-based tracking supported by an artificial intelligence net (AI net), and the software Crush, that uses a completely AI-based method. For this purpose, all motion data was recorded with two in-house motion capture systems. One system for recording the movements for a markerbased evaluation as gold standard and one system for markerless tracking. Within a laboratory environment, eight cameras per system were mounted around the area of motion. By placing two cameras in the same position and using the same calibration, deviations in the image data between those for markerbased and markerless tracking were extremely minimal. Based on this data, markerbased tracking was performed using the Simi Motion program, markerless tracking was performed using the Simi Shape software system and the latest software from Simi Reality Motion Systems, Crush. When comparing the markerless data with the markerbased data, an average root mean square error of 0,038 m was calculated for Simi Shape and a deviation of 0,037 m for Crush. In a direct comparison of the two markerless systems, a root mean square error of 0,019 m was scored. Based on these data, conclusions could be drawn about the accuracies of the two markerless systems. The obtained kinematic data of the tracking are in the range of high accuracy, which is limited to a deviation of less than 0,05 m according to the literature.
The effects of climate change, including severe storms, heat waves, and melting glaciers, are highlighted as an urgent concern, emphasising the need to decrease carbon emissions to restrict global warming to 1.5°C. To accomplish this goal, it is vital to substitute fossil fuel-based power plants with renewable energy sources like solar, wind, hydro, and biofuels. Despite some progress being made, the proportion of renewables used in generating electricity is still lower than the levels needed for 2030 and 2050. Decarbonising the power grid is also critical in lowering the energy consumption of buildings, which is responsible for a substantial percentage of worldwide electricity usage. Even though there has been substantial expansion in the worldwide renewable energy market in the past 15 years, the transition to renewable energy sources also requires taking into account the importance of energy trading.
Peer-to-peer (P2P) electricity trading is an emerging type of energy exchange that can revolutionise the energy sector by providing a more decentralised and efficient way of trading energy. This research deals about P2P electricity trading in a carbon-neutral scenario. 'Python for Power System Analysis' (PyPSA) was used to develop models through which the P2P effect was tested. Data for the entire state of Baden-Württemberg (BW) was collected. Three scenarios were taken into consideration while developing models: 2019 (base), 2030 (coal phase-out), and 2040(climate neutral). Alongside this, another model with no P2P trading was developed to make a comparison. In addition, the use case of community storage in a P2P trading network is also presented.
The research concludes that P2P has a significant positive effect on a pathway to achieve climate neutrality. The findings show that the share of renewables in electricity generation is increasing compared to conventional sources in BW, which can be traded to meet the demand. From the storage analysis, it can be concluded that community storage can be effectively utilised in P2P trading. While the emissions are reduced, the operating costs are also reduced when the grid has P2P trading available. By highlighting the benefits of P2P trading, this research contributed to the growing body of research on the effectiveness of P2P trading in an electricity network grid.
To date, many experiments have been performed to study how the internal geometrical shapes of the annular liquid seal can reduce internal leakage and increase pump efficiency. These can be time-consuming and expensive as all rotordynamic coefficients must be determined in each case.
Nowadays, accurate simulation methods to calculate rotordynamic coefficients of annular seals are still rare. Therefore, new numerical methods must be designed and validated for annular seals.
The present study aims to contribute to this labour by providing a summary of the available test rig and seals dimensions and experimental results obtained in the following experiments:
− Kaneko, S et al., Experimental Study on Static and Dynamic Characteristics of Liquid Annular Convergent-Tapered Seals with Honeycomb Roughness Pattern (2003) [1] − J. Alex Moreland, Influence of pre-swirl and eccentricity in smooth stator/grooved rotor liquid annular seals, static and rotordynamic characteristics (2016) [2]
A 3D CAD simulation with Siemens NX Software of the test rig used in J. Alex Moreland’s experiment has been made. The following annular liquid seals have also been 3D modelled, as well as their fluid volume:
− Smooth Annular Liquid Seal (SS/GR) (J. Alex Moreland experiment)
− Grooved Annular Liquid Seal (GS/SR)
− Round-Hole Pattern Annular Liquid Seal (𝐻𝑑=2 mm) (GS/SR)
− Straight Honeycomb Annular Liquid Seal (GS/SR)
− Convergent Honeycomb Annular Liquid Seal (No. 3) (GS/SR)
− Smooth Annular Liquid Seal (SS/SR) (S. Kaneko experiment)
In the case of the seals used in S. Kaneko’s experiments, the test rig has been adapted to each seal, defining interpart expressions which can be easily modified.
Afterwards, it has been done a CFD simulation of the Smooth Annular Liquid Seal using Ansys CFX Software. To do so, the fluid volume geometry has been simplified to do a first approximation. Results have been compared for an eccentricity 𝜀0=0.00 for the following ranges of rotor speeds and differential of pressure:
− Δ𝑃= 2.07, 4.14, 6.21, and 8.27 bar,
− 𝜔= 2, 4, 6 and 8 krpm.
Even results obtained have the same trend as the one proportionated by the literature, they cannot be validated as the error is above 5%. It is also observed that as the pressure drop increases, the relative error decreases considerably.