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With recent developments in the Ukrainian-Russian conflict, many are discussing about Germany’s dependency on fossil fuel imports in its energy system, and how can the country proceed with reducing that dependency. With its wide-ranging consumption sectors, the electricity sector comes as the perfect choice to start with. Recent reports showed that the German federal government is already intending to have a fully renewable electricity by 2035 while exploiting all possible clean power options. This was published in the federal government’s climate emergency program (Easter Package) in early 2022. The aim of this package is to initiate a rapid transition and decarbonization of the electricity sector. The Easter Package expects an enormous growth of renewable energies to a completely new level, with already at least 80% renewable gross energy consumption, with extensive and broad deployment of different generation technologies on various scales. This paper will discuss this ambitious plan and outline some insights into this huge and rapidly increasing step, and show how much will Germany need in order to achieve this huge milestone towards a fully green supply of the electricity sector. Different scenarios and shares of renewables will be investigated in order to elaborate on preponed climate-neutral goal of the electricity sector by 2035. The results pointed out some promising aspects in achieving a 100% renewable power, with massive investments in both generation and storage technologies.
BACKGROUND
Various neutral and alkaline peptidases are commercially available for use in protein hydrolysis under neutral to alkaline conditions. However, the hydrolysis of proteins under acidic conditions by applying fungal aspartic peptidases (FAPs) has not been investigated in depth so far. The aim of this study, thus, was to purify a FAP from the commercial enzyme preparation, ROHALASE® BXL, determine its biochemical characteristics, and investigate its application for the hydrolysis of food and animal feed proteins under acidic conditions.
RESULTS
A Trichoderma reesei derived FAP, with an apparent molecular mass of 45.8 kDa (sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SDS-PAGE) was purified 13.8-fold with a yield of 37% from ROHALASE® BXL. The FAP was identified as an aspartate protease (UniProt ID: G0R8T0) by inhibition and nano-LC-ESI-MS/MS studies. The FAP showed the highest activity at 50°C and pH 4.0. Monovalent cations, organic solvents, and reducing agents were tolerated well by the FAP. The FAP underwent an apparent competitive product inhibition by soy protein hydrolysate and whey protein hydrolysate with apparent Ki-values of 1.75 and 30.2 mg*mL−1, respectively. The FAP showed promising results in food (soy protein isolate and whey protein isolate) and animal feed protein hydrolyses. For the latter, an increase in the soluble protein content of 109% was noted after 30 min.
CONCLUSION
Our results demonstrate the applicability of fungal aspartic endopeptidases in the food and animal feed industry. Efficient protein hydrolysis of industrially relevant substrates such as acidic whey or animal feed proteins could be conducted by applying fungal aspartic peptidases. © 2022 Society of Chemical Industry.
Linear acceleration is a key performance determinant and major training component of many sports. Although extensive research about lower limb kinetics and kinematics is available, consistent definitions of distinctive key body positions, the underlying mechanisms and their related movement strategies are lacking. The aim of this ‘Method and Theoretical Perspective’ article is to introduce a conceptual framework which classifies the sagittal plane ‘shin roll’ motion during accelerated sprinting. By emphasising the importance of the shin segment’s orientation in space, four distinctive key positions are presented (‘shin block’, ‘touchdown’, ‘heel lock’ and ‘propulsion pose’), which are linked by a progressive ‘shin roll’ motion during swing-stance transition. The shin’s downward tilt is driven by three different movement strategies (‘shin alignment’, ‘horizontal ankle rocker’ and ‘shin drop’). The tilt’s optimal amount and timing will contribute to a mechanically efficient acceleration via timely staggered proximal-to-distal power output. Empirical data obtained from athletes of different performance levels and sporting backgrounds are required to verify the feasibility of this concept. The framework presented here should facilitate future biomechanical analyses and may enable coaches and practitioners to develop specific training programs and feedback strategies to provide athletes with a more efficient acceleration technique.
Bud type carbon nanohorns (CNHs) are composed of carbon and have a closed conical tip at one end protruding from an aggregate structure. By employing a simple oxidation process in CO2 atmosphere, it is possible to open the CNH tips which increases their specific surface area by four fold. These tip opened CNHs combine the microporous nature of activated carbons and the crystalline mesoporous character of carbon nanotubes. The results for the high pressure CO2 gas adsorption of tip opened CNHs are reported herein for the first time and are found to be superior to traditional CO2 adsorbents like zeolites. The modified CNHs are also found to be promising materials for lithium ion batteries and the performance is found to be on a par with carbon nanotubes and carbon nanofibers.
In this paper, the J-integral is derived for temperature-dependent elastic–plastic materials described by incremental plasticity. It is implemented using the equivalent domain integral method for assessment of three-dimensional cracks based on results of finite-element calculations. The J-integral considers contributions from inhomogeneous temperature fields and temperature-dependent elastic and plastic material properties as well as from gradients in the plastic strains and the hardening variables. Different energy densities are considered, the Helmholtz free energy and the stress-working density, providing a physical meaning of the J-integral as a fracture criteria for crack growth. Results obtained for a plate with two different crack configurations each loaded by a cool-down thermal shock show domain-independence of the incremental J-integral for different energy densities even for high temperature gradients and significant temperature-dependence of the yield stress and the hardening exponent in the presence of large scale yielding. Hence, the derived J-integral is an appropriate parameter for the assessment of cracks in thermomechanically loaded components.
In thin-layer chromatography, fiber-bundle arrays have been introduced for spectral absorption measurements in the UV-region. Using all-silica fiber bundles, the exciting light will be detected after re-emission on the plate with a fiberoptic spectrometer. In addition, fluorescence light can be detected which will be masked by the re-emitted light. Therefore, it is helpful to separate the absorption and fluorescence on the TLC-plate. A modified three-array assembly has been developed: using one array for detection, the two others are used for excitation with broadband band deuterium-light and with UV-LEDs adjusted to the substances under test. As an example, the quantification of glucosamine in nutritional supplements or spinach leaf extract will be described. Using simply heating of the amino-plate for derivation, the reaction product of Glucosamine can be detected sensitively either by light absorption or by fluorescence, using the new fiber-optic assembly. In addition, the properties of the new 3-row fiber-optic array and the commercially available UV-LEDs will be shown, in the interesting wavelength region for excitation of fluorescence, from 260 nm to 360 nm. The squint angle having an influence on coupling efficiency and spatial resolution will be measured with the inverse farfield method. Some properties of UV-LEDs for analytical applications will be described and discussed, too.
Background
To assess the in-field walking mechanics during downhill hiking of patients with total knee arthroplasty five to 14 months after surgery and an age-matched healthy control group and relate them to the knee flexor and extensor muscle strength.
Methods
Participants walked on a predetermined hiking trail at a self-selected, comfortable pace wearing an inertial sensor system for recording the whole-body 3D kinematics. Sagittal plane hip, knee, and ankle joint angles were evaluated over the gait cycle at level walking and two different negative slopes. The concentric and eccentric lower extremity muscle strength of the knee flexors and extensors isokinetically at 50 and 120°/s were measured.
Findings
Less knee flexion angles during stance have been measured in patients in the operated limb compared to healthy controls in all conditions (level walking, moderate downhill, steep downhill). The differences increased with steepness. Muscle strength was lower in patients for both muscle groups and all measured conditions. The functional hamstrings to quadriceps ratio at 120°/sec correlated with knee angle during level and downhill walking at the moderate slope in patients, showing higher ratios with lower peak knee flexion angles.
Interpretation
The study shows that even if rehabilitation has been completed successfully and complication-free, five to 14 months after surgery, the muscular condition was still insufficient to display a normal gait pattern during downhill hiking. The muscle balance between quadriceps and hamstring muscles seems related to the persistence of a stiff knee gait pattern after knee arthroplasty. LoE: III.
The use of renewable energy sources for heating and cooling in buildings today offers the best opportunities to avoid the use of fossil fuels and the associated climate-damaging emissions. However, unlike fossil fuels, renewable energy sources such as solar radiation are not available at the push of a button, but occur uncontrollably depending on weather conditions, the location of the building and the time of year. Their use is free of charge. However, complex converters and systems usually have to be installed in order to use them. These must be carefully planned and operated in order to avoid unnecessary costs and to generate the maximum possible yield. The regenerative energy systems are usually integrated into existing conventional systems. When designing the control and regulation equipment, it is crucial to design the automation of the systems in such a way that primarily renewable energy sources are used and the share of fossil energy sources is minimized.
Automation devices or automation stations (AS) take on the task of controlling, regulating, monitoring and, if necessary, optimising building systems and their system components (e.g. pumps, compressors, fans) based on recorded process variables. For this purpose, a wide range of control and regulation methods are used, starting with simple on/off controllers, through classic PID controllers, to higher-order controllers such as adaptive, model-predictive, knowledge-based or adaptive controllers.
Starting with a brief introduction to automation technology (Sect. 7.1), the chapter goes into the structure and functionality of the usual compact controllers using the application examples of solar thermal systems and heat pump systems (Sect. 7.2). Finally, the integration of system automation into a higher-level building automation system and into the building management system is described using specific application examples (Sect. 7.3).
This central book chapter now details the implementation of automation of solar domestic hot water systems, solar assisted building heating, rooms, solar cooling systems, heat pump heating systems, geothermal systems and thermally activated building component systems. Hydraulic and automation diagrams are used to explain how the automation of these systems works. A detailed insight into the engineering and technical interrelationships involved in the use of these systems, as well as the use of simulation tools, enables effective control and regulation. System characteristic curves and systematic procedures support the automation engineer in his tasks.
Renewable energy sources such as solar radiation, geothermal heat and ambient heat are available for energy conversion. With the help of special converters, these resources can be put to use. These include solar collectors, geothermal probes and chillers. They collect the energy and convert it to a temperature level high enough to be suitable for heat purposes. In the case of refrigeration machines, a distinction is made between electrically and thermally driven machines.
Vortex breakdown phenomena in rotating fluids are investigated both theoretically and experimentally. The fluid is contained in a cone between two spherical surfaces. The primary swirling motion is induced ba the rotating lower boundary. The upper surface can be fixed with non-slip condition or can be a stress-free surface. Depending on these boundary conditions and on the Reynolds number, novel structures of recirculation zones are realized. The axisymmetric flow patterns are simulated numerically by a finite difference method. Experiments are done to visualize the topological structure of the flow pattern and to observe the existence ranges of the different recirculating flows. The comparison between theory and experiment shows good agreement with respect to the topological structure of the flow.
In this paper we report on further success of our work to develop a multi-method energy optimization which works with a digital twin concept. The twin concept serves to replicate production processes of different kinds of production companies, including complex energy systems and test market interactions to then use them for model predictive optimizing. The presented work finally reports about the performed flexibility assessment leading to a flexibility audit with a list of measures and the impact of energy optimizations made related to interactions with the local power grid i.e., the exchange node of the low voltage distribution grid. The analysis and continuous exploration of flexibilities as well as the exchange with energy markets require a “guide” leading to continuous optimization with a further tool like the Flexibility Survey and Control Panel helping decision-making processes on the day-ahead horizon for real production plants or the investment planning to improve machinery, staff schedules and production
infrastructure.
Polyarticulated active prostheses constitute a promising solution for upper limb amputees. The bottleneck for their adoption though, is the lack of intuitive control. In this context, machine learning algorithms based on pattern recognition from electromyographic (EMG) signals represent a great opportunity for naturally operating prosthetic devices, but their performance is strongly affected by the selection of input features. In this study, we investigated different combinations of 13 EMG-derived features obtained from EMG signals of healthy individuals performing upper limb movements and tested their performance for movement classification using an Artificial Neural Network. We found that input data (i.e., the set of input features) can be reduced by more than 50% without any loss in accuracy, while diminishing the computing time required to train the classifier. Our results indicate that input features must be properly selected in order to optimize prosthetic control.
Due to the Covid-19 pandemic, the RoboCup WorldCup 2021 was held completely remotely. For this competition the Webots simulator (https://cyberbotics.com/) was used, so all teams needed to transfer their robot to the simulation. This paper describes our experiences during this process as well as a genetic learning approach to improve our walk engine to allow a more stable and faster movement in the simulation. Therefore we used a docker setup to scale easily. The resulting movement was one of the outstanding features that finally led to the championship title.
Previous studies of the hyphenation of gas chromatographic separation and spectrophotometric detection in the ultraviolet wavelength range between 168 and 330 nm showed a high potential for applications where the analysis of complex samples is required. Within this paper the development of a state-of-the-art detection system for compounds in the vapour phase is described, offering an improved behaviour compared to previous systems: Dependent on the requirements of established detection systems hyphenated with gas chromatography, the main components of the system have to be designed for optimum performance and reliability of the spectrophotometric detector: A deuterium lamp as a broadband light source has been selected for improved stability in the measurements. A new-type absorption cell based on fiber-optics has been developed considering the dynamic necessary to compete with existing techniques. In addition, the influence of the volume of the cell on the chromatogram needs to be analyzed. Tests for determining the performance of the absorption cell in terms of chemical and thermal influences have been carried out. A new spectrophotometer with adequate spectral resolution in the wavelength range, offering improved stability and dynamic for an efficient use in this application was developed. Furthermore, the influence of each component on the performance, reliability and stability of the sensor system will be discussed. An overview and outlook over the potential applications in the environmental, scientific and medical field will be given.
Energy efficiency and hygrothermal performance of hemp clay walls for Moroccan residential buildings
(2023)
Hemp-based building envelopes have gained significant popularity in developed countries, and now the trend of constructing houses with hemp-clay blocks is spreading to developing countries like Morocco. Investigating the hygrothermal behavior of such structures under actual climate conditions is essential for advancing and promoting this sustainable practice. This paper presents an in-depth experimental characterization of a commercial hemp-clay brick that has been exposed to the outdoor environment for four years, in addition to field measurements on a building scale demonstration prototype. Additionally, the study simulates 17 representative cities to assess the hygrothermal performance and energy-saving potential in each of Morocco's six existing climate zones, using the EnergyPlus engine. The experimental campaign's findings demonstrate excellent indoor air temperature and relative humidity regulation within the hemp-clay wall building, leading to satisfactory levels of thermal comfort within hemp-clay wall buildings. This is attributed to the material's good thermal conductivity and excellent moisture buffering capacity (found to be 0.31 W/mK and 2.25 g/m2%RH), respectively). The energy simulation findings also point to significant energy savings, with cooling and heating energy reductions ranging from 27.7% to 47.5% and 33.7% to 79.8%, respectively, as compared to traditional Moroccan buildings.
To improve the building’s energy efficiency many parameters should be assessed considering the building envelope, energy loads, occupation, and HVAC systems. Fenestration is among the most important variables impacting residential building indoor temperatures. So, it is crucial to use the most optimal energy-efficient window glazing in buildings to reduce energy consumption and at the same time provide visual daylight comfort and thermal comfort. Many studies have focused on the improvement of building energy efficiency focusing on the building envelope or the heating, ventilation, and cooling systems. But just a few studies have focused on studying the effect of glazing on building energy consumption. Thus, this paper aims to study the influence of different glazing types on the building’s heating and cooling energy consumption. A real case study building located under a semi-arid climate was used. The building energy model has been conducted using the OpenStudio simulation engine. Building indoor temperature was calibrated using ASHRAE’s statistical indices. Then a comparative analysis was conducted using seven different types of windows including single, double, and triple glazing filled with air and argon. Tripleglazed and double-glazed windows with argon space offer 37% and 32% of annual energy savings. It should be stressed that the methodology developed in this paper could be useful for further studies to improve building energy efficiency using optimal window glazing.
This review provides an overview on the production and analysis techniques of antioxidative peptides from food proteins. Regarding the production of antioxidative peptides, interlinked factors must be considered. Depending on the protein substrate, different peptidases or peptidase systems containing multiple enzymes as well as a specific production process must be chosen. The antioxidative peptides might be produced in a batch process including multiple pre- and post-treatments, besides the hydrolyses with peptidases itself. As an alternative, the potential of continuous production systems is discussed in this review. Furthermore, robust analyses tools are needed to gain control of the process and final product properties. With no standardized methodology available for antioxidative peptide evaluation, pros and cons of various strategies for peptide separation and antioxidative measurement are discussed in this review. Therefore, this review provides a roadmap for antioxidative peptide generation from various sources for research and development as well as for potential industrial use.