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Do you know that for each banana bunch the complete plant must be cut as well? Only in Brazil 440 million trees are planted annually. With an average weight of 30 kg per banana plant you can estimate about 13,5 million tons of banana residues per year. Although there exist some projects to use these residues for the production of valuable products (e.g fibers for textile and paper production) most of this organic waste material is unused and left for composting on the farmland.
The basic idea of this project is to evaluate this organic waste material for converting it to a renewable and CO2 neutral fuel. Therefore, the different parts of the banana plant (heart, leaves and pseudo stem) were analyzed regarding their biogas potential (specific biogas yield and biogas production kinetics). In further studies the effect of mechanical and enzymatic pretreatments of the different parts of the plants was investigated. This examination could then be the basis for an energetic usage of this organic residue.
The biogas batch experiments were performed according to the german guideline VDI 4630 in 2-L-Batch reactors at 37°C. As biogas substrates, the heart, the leaves and the pseudo stem of the banana plant residue with and without enzymatic/mechanical pretreatment were used.
The different parts of the banana plants result in a specific biogas production yield in the range of 260-470 norm liters per kg organic dry mass.
To determine the influence of the mechanical pretreatment (particle size 1-15 mm) on the biogas production kinetics, the kinetic constants were defined and calculated. The reduction of the particle size leads to an improved biogas production kinetics. Therefore experiments will demonstrate, if the results from the batch experiments can be converted in the continuous fed biogas reactor. The experiments of the enzymatic pretreatment are still under investigation.
Die angestrebten Klimaschutzziele erfordern, dass Erneuerbare Energien längerfristig zur Hauptenergiequelle der Energieversorgung werden. Um dieses ehrgeizige Ziel zu erreichen, ist es angebracht konventionelle und erneuerbare Energie oder noch besser nachhaltige Einzelprozesse intelligent miteinander zu verknüpfen.
Das Projekt EBIPREP wird von einer interdisziplinären Forschergruppe bestehend aus Chemikern, Prozessingenieuren und Bioprozessingenieuren sowie Physikern, die auf Sensoren und Prozesssteuerung spezialisiert sind durchgeführt. Das Ziel ist es, neue Lösungen für die Nutzungswege von Holzhackschnitzeln und den bei der mechanischen Trocknung anfallenden Holzpresssaft zu entwickeln. Neben der Hackschnitzelvergasung und der katalytischen Reinigung des Holzgases steht die Nutzung des Holzpresssafts in Biogasanlagen und bei der biotechnologischen Wertstofferzeugung, z.B. bei der Enzymherstellung, im Vordergrund.
Was wir tun?
Das EBIPREP-Projekt wird von einer interdisziplinären Forschungsgruppe durchgeführt, die sich aus Chemikern, Prozessingenieuren, Bioprozessingenieuren und Physikern zusammensetzt. Ziel ist es, neue Lösungen für den Einsatz von Hackschnitzeln und Holzpresssaft zu entwickeln, die durch ein innovatives mechanisches Trocknungsverfahren gewonnen werden. Neben der Holzvergasung und katalytischen Reinigung des Holzgases ist der Einsatz von Holzpresssaft in Biogasanlagen und in biotechnologischen Produktionsprozessen von Wertstoffen vorgesehen. Holzhackschnitzel werden thermisch vergast. Es werden Online-Sensoren entwickelt, um die relevanten Parameter der stabilisierten und optimierten Einzelprozesse auszuwerten. Die Verknüpfung von thermischen und biotechnologischer Konversionsprozessen könnte dazu beitragen, die Dimension von Biogasreaktoren erheblich zu reduzieren. Diese Tatsache wird folglich zu einer spürbaren Kostensenkung führen.
Ziele des EBIPREP-Projekts
• die Vorteile der thermischen und biologischen Umwandlung von Biomasse zu kombinieren;
• Entwicklung eines Verfahrens zur Reduzierung von Schadstoffemissionen mit innovativen Sensoren und katalytische Behandlung von Synthesegasen;
• nachhaltige Produktion biotechnologischer wertvoller Produkte
• wirtschaftliche und ökologische Analyse des Gesamtprozesses im Vergleich zu den Einzelprozessen
• Einsatz von Prozessabwässern zur Erzeugung regenerativer Energie oder biotechnologischer Wertstoffe
• Erwerb neuer Kenntnisse auf dem Gebiet der Rückgewinnungstechnik von Rückständen
• und Energieerzeugung;
• Erweiterung neuer Anwendungsfelder für innovative Sensoren und Keramik
• Schäume für Katalysatoren;
• Senkung der Kosten für die Biogasproduktion
Im geplanten Übersichtsvortrag werden die vernetzten Strukturen des Projekts EBIPREP und deren zentralen Ergebnisse vorgestellt.
Das Ziel dieses Projekts ist die Entwicklung und Charakterisierung einer mikrobiellen Brennstoffzelle (MBZ). Die MBZ unterscheidet sich von einer herkömmlichen Brennstoffzelle darin, dass die an der Anode erzeugten Elektronen nicht vom molekularen Wasserstoff, sondern direkt von der im Anodenkompartiment wachsenden Biomasse aus organischen Verbindungen stammen. Die Funktionsweise einer solchen Zelle ist in Abbildung 3.4-1 dargestellt. Im Gegensatz zur herkömmlichen Brennstoffzelle können in einer MBZ auch Abwasserteilströme z. B. aus der Lebensmittelindustrie als Substrat eingesetzt werden. Der große Vorteil der MBZ besteht somit darin, dass Abwässer biologisch abgebaut und gleichzeitig elektrischer Strom erzeugt werden kann.
In contrast to a conventional fuel cell the electrons in a microbial fuel cell (MFC) originate from the metabolic conversion of organic substrates by special bacteria instead of using molecular hydrogen. Recent research in our group has shown that the maximum electrical power density in a MFC correlates with the biomass concentration in batch MFC experiments. In continuous MFC systems additionally the dilution rate D could have an effect on the specific power density. Therefore two steady state conditions are adjusted and the resulting specific power densities, and the biomass and substrate concentrations were measured. These results were implemented in a mathematical description of the continuous MFC-process and the visualization of the model is presented.
Die biologische Verwertung von cellulose-/ hemicellulose- und lignocellulosereichen organischen Substraten zur Erzeugung von Energieträgern gewinnt zunehmend an Bedeutung. Im Gegensatz zu Biokraftstoffen der ersten Generation, bei denen nur ein kleiner Teil des pflanzlichen Materials eingesetzt worden ist (Öl, Zucker, Stärke), wird bei Biokraftstoffen der zweiten Generation fast die vollständige Pflanze einschließlich der schwer zugänglichen Cellulose verwendet. In Biogasanlagen führt diese Zielstellung jedoch häufig zu Problemen. Lignocellulose-reiches Material ist für viele Mikroorganismen schwer oder gar nicht abbaubar. Um die schwer abbaubaren Pflanzenteile wie Cellulose, Hemicellulose oder Lignin den Mikroorganismen in einer Biogasanlage besser zugänglich zu machen, können Biogassubstrate vorbehandelt werden.
Numerous 2,5-dimethoxy-N-benzylphenethylamines (NBOMe), carrying a variety of lipophilic substituents at the 4-position, are potent agonists at 5-hydroxytryptamine (5HT2A ) receptors and show hallucinogenic effects. The present study investigated the metabolism of 25D-NBOMe, 25E-NBOMe, and 25N-NBOMe using the microsomal model of pooled human liver microsomes (pHLM) and the microbial model of the fungi Cunninghamella elegans (C. elegans). Identification of metabolites was performed using liquid chromatography-high resolution-tandem mass spectrometry (LC-HR-MS/MS) with a quadrupole time-of-flight (QqToF) instrument. In total, 36 25D-NBOMe phase I metabolites, 26 25E-NBOMe phase I metabolites and 24 25N-NBOMe phase I metabolites were detected and identified in pHLM. Furthermore, 14 metabolites of 25D-NBOMe, 11 25E-NBOMe metabolites, and nine 25N-NBOMe metabolites could be found in C. elegans. The main biotransformation steps observed were oxidative deamination, oxidative N-dealkylation also in combination with hydroxylation, oxidative O-demethylation possibly combined with hydroxylation, oxidation of secondary alcohols, mono- and dihydroxylation, oxidation of primary alcohols, and carboxylation of primary alcohols. Additionally, oxidative di-O-demethylation for 25E-NBOMe and reduction of the aromatic nitro group and N-acetylation of the primary aromatic amine for 25N-NBOMe took place. The resulting 25N-NBOMe metabolites were unique for NBOMe compounds. For all NBOMes investigated, the corresponding 2,5-dimethoxyphenethylamine (2C-X) metabolite was detected. This study reports for the first time 25X-NBOMe N-oxide metabolites and hydroxylamine metabolites, which were identified for 25D-NBOMe and 25N-NBOMe and all three investigated NBOMes, respectively. C. elegans was capable of generating all main biotransformation steps observed in pHLM and might therefore be an interesting model for further studies of new psychoactive substances (NPS) metabolism.
Tryptamines can occur naturally in plants, mushrooms, microbes, and amphibians. Synthetic tryptamines are sold as new psychoactive substances (NPS) because of their hallucinogenic effects. When it comes to NPS, metabolism studies are of crucial importance, due to the lack of pharmacological and toxicological data. Different approaches can be taken to study in vitro and in vivo metabolism of xenobiotica. The zygomycete fungus Cunninghamella elegans (C. elegans) can be used as a microbial model for the study of drug metabolism. The current study investigated the biotransformation of four naturally occurring and synthetic tryptamines [N,N‐Dimethyltryptamine (DMT), 4‐hydroxy‐N‐methyl‐N‐ethyltryptamine (4‐HO‐MET), N,N‐di allyl‐5‐methoxy tryptamine (5‐MeO‐DALT) and 5‐methoxy‐N‐methyl‐N‐isoporpoyltryptamine (5‐MeO‐MiPT)] in C. elegans after incubation for 72 hours. Metabolites were identified using liquid chromatography–high resolution–tandem mass spectrometry (LC–HR–MS/MS) with a quadrupole time‐of‐flight (QqTOF) instrument. Results were compared to already published data on these substances. C. elegans was capable of producing all major biotransformation steps: hydroxylation, N‐oxide formation, carboxylation, deamination, and demethylation. On average 63% of phase I metabolites found in the literature could also be detected in C. elegans. Additionally, metabolites specific for C. elegans were identified. Therefore, C. elegans is a suitable complementary model to other in vitro or in vivo methods to study the metabolism of naturally occurring or synthetic tryptamines.
The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fedbatch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source. The biomass value reached 3.0 g/l of CDW with 20% of PHAs content within 48 h of cultivation. The polymer was purified from lyophilized cells and analyzed by gas chromatography (GC). The results revealed that the monomeric composition of the obtained polyesters depended on the available substrate. When glucose was used in the growth phase, 3-hydroxyundecanoate and 3- hydroxydodecanoate were found in the polymer composition, whereas in the PHAs-accumulating stage, the Pseudomonas Gl01 strain synthesized mcl-PHAs consisting mainly of 3- hydroxyoctanoate and 3-hydroxydecanoate. The transcriptional analysis using reverse-transcription real-time PCR reaction revealed that the phaC1 gene could be transcribed simultaneously to the phaZ gene.
The identification and quantification of compounds in the gas phase becomes of increasing interest in the context of environmental protection, as well as in the analytical field. In this respect, the high extinction coefficients of vapours and gases in the ultraviolet wavelength region allow a very sensitive measurement system. In addition, the increased performance of the components necessary for setting up a measurement system, such as fibres, light sources and detectors has been improved. In particular the light sources and detectors offer improved stability, and the deep UV performance and solarisation resistance of fused silica fibres allow have been significantly optimized in the past years. Therefore a compact and reliable detection system with high measuring accuracy is developed. Within this paper possible applications of the system under development and recent results will be discussed.
Wood juice, a liquid produced during wood processing, is a harmful waste that requires utilization. To achieve a circular economy, biowastes should be recycled, reducing fossil carbon usage. Therefore, the objective of this work was to examine the potential of wood juice as a feedstock for bioplastic synthesis by Bacillus sp. G8_19. Polyhydroxyalkanoate (PHA) syntheses using wood juice from Douglas fir trees and that from a mixture of spruce/fir trees were compared. It was found that the PHA content was higher after using wood juice from spruce/fir trees than that from Douglas fir trees (18.0% vs 6.1% of cell dry mass). Gas chromatography analysis showed that, with both wood juices, Bacillus sp. G8_19 accumulated poly(3-hydroxybutyrate-co-3-hydroxyvalerate). The content of 3-hydroxyvalerate (3HV) monomers was higher when spruce/fir wood juice was used (10.7% vs 1.9%). The C/N ratio did not have a statistically significant effect on the copolymer content in biomass, but it did significantly influence the 3HV content. The proposed concept may serve as an approach to wood waste valorization via production of biodegradable materials.