Fiber Optical Scanning in TLC by Use of a Diode-Array Detector — Linearization Models for Absorption and Fluorescence Evaluation
- High performance thin layer chromatography (HPTLC) is a frequently used separation technique which works well for quantification of caffeine and quinine in beverages. Competing separation techniques, e.g. high-performance liquid chromatography (HPLC) or gas chromatography (GC), are not suitable for sugar-containing samples, because these methods need special pretreatment by the analyst. In HPTLC,High performance thin layer chromatography (HPTLC) is a frequently used separation technique which works well for quantification of caffeine and quinine in beverages. Competing separation techniques, e.g. high-performance liquid chromatography (HPLC) or gas chromatography (GC), are not suitable for sugar-containing samples, because these methods need special pretreatment by the analyst. In HPTLC, however, it is possible to separate ‘dirty’ samples without time-consuming pretreatment, because disposable HPTLC plates are used. A convenient method for quantification of caffeine and quinine in beverages, without sample pretreatment, is presented below. The basic theory of in-situ quantification in HPTLC by use of remitted light is introduced and discussed. Several linearization models are discussed. A home-made diode-array scanner has been used for quantification; this, for the first time, enables simultaneous measurements at different wavelengths. The new scanner also enables fluorescence evaluation without further equipment. Simultaneous recording at different wavelengths improves the accuracy and reliability of HPTLC analysis. These aspects result in substantial improvement of in-situ quantitative densitometric analysis and enable quantification of compounds in beverages.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/7291 | Bibliografische Angaben |
Title (English): | Fiber Optical Scanning in TLC by Use of a Diode-Array Detector — Linearization Models for Absorption and Fluorescence Evaluation |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Peter Post, Siegfried Ebel |
Year of Publication: | 2002 |
Publisher: | Akadémiai Kiadó |
First Page: | 88 |
Last Page: | 93 |
Parent Title (English): | JPC : Journal of Planar Chromatography - Modern TLC |
Volume: | 15 |
Issue: | 2 |
ISSN: | 1789-0993 (Online) |
ISSN: | 0933-4173 (Print) |
DOI: | https://doi.org/10.1556/JPC.15.2002.2.1 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Tag: | Diode-array detection; Kubelka-Munk theory; Linearization models; TLC scanner; Thin-layer chromatography | Formale Angaben |
Open Access: | Closed |
Licence (German): | Urheberrechtlich geschützt |