Theory and Instrumentation for In situ Detection
- The main focus of this chapter is the theoretical and instrumental processes that underpin densitometric methods widely used in thin-layer chromatography (TLC). Densitometric methods include UV–vis, luminescence and fluorescence optical measurements as well as infrared and Raman spectroscopic measurements. The chapter is divided in two general parts: a theoretical part and a practical part. TheThe main focus of this chapter is the theoretical and instrumental processes that underpin densitometric methods widely used in thin-layer chromatography (TLC). Densitometric methods include UV–vis, luminescence and fluorescence optical measurements as well as infrared and Raman spectroscopic measurements. The chapter is divided in two general parts: a theoretical part and a practical part. The systems for direct radioactivity measurements and the combination of TLC with mass spectrometry are also discussed. All these systems allow measuring an intensity distribution directly on a TLC plate. We call this “in situ detection” because no analyte is removed from the plate.…
Document Type: | Part of a Book |
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Zitierlink: | https://opus.hs-offenburg.de/8409 | Bibliografische Angaben |
Title (English): | Theory and Instrumentation for In situ Detection |
Author: | Bernd SpangenbergStaff MemberORCiDGND |
Year of Publication: | 2015 |
Publisher: | Elsevier |
First Page: | 167 |
Last Page: | 189 |
Parent Title (English): | Instrumental Thin-Layer Chromatography |
Editor: | Colin F. Poole |
ISBN: | 978-0-12-417223-4 (Print) |
ISBN: | 978-0-12-417284-5 (eBook) |
DOI: | https://doi.org/10.1016/B978-0-12-417223-4.00007-8 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie | Formale Angaben |
Open Access: | Closed |
Licence (German): | Urheberrechtlich geschützt |
Comment: | Nachauflage: 2. Auflage, 2023 |