Quantitative Two-Dimensional Thin-Layer Chromatography Using a Diode-Array Detector
- We will present the first example of a two-dimensional scanned TLC-plate, measured by use of a diode-array scanner. A spatial resolution of 250 µm was achieved on plate. The system provides real 2D fluorescence and absorption spectra in the wavelength-range from 190 to 1000 nm with a spectral resolution of greater than 1 nm. A mixture of 12 sulphonamides was separated by using a cyanopropyl-coatedWe will present the first example of a two-dimensional scanned TLC-plate, measured by use of a diode-array scanner. A spatial resolution of 250 µm was achieved on plate. The system provides real 2D fluorescence and absorption spectra in the wavelength-range from 190 to 1000 nm with a spectral resolution of greater than 1 nm. A mixture of 12 sulphonamides was separated by using a cyanopropyl-coated silica gel plate (Merck, 1.16464) with the solvent mix of methyl tert-butyl ether-methanol-dichloromethane-cyclohexane-NH3 (25%) (48:2:2:1:1, v/v) in the first and with a mixture of water-acetonitrile-dioxane-ethanol (8:2:1:1, v/v) in the second direction. Both developments were carried out over a distance of 70 mm. A separation number (spot capacity) of 259 was calculated. We discussed a new formula for its calculation in 2D-TLC separations. The drawback of this method is that measuring a 2D-TLC plate needs more than 3 h measurement time.…
Document Type: | Conference Proceeding |
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Conference Type: | Konferenzartikel |
Zitierlink: | https://opus.hs-offenburg.de/7287 | Bibliografische Angaben |
Title (English): | Quantitative Two-Dimensional Thin-Layer Chromatography Using a Diode-Array Detector |
Conference: | International Symposium on Separation Sciences (17. : September 5-9, 2011 : Cluj-Napoca, Romania) |
Author: | Barbara MilzStaff MemberGND, Karl-Friedrich Klein, Bernd SpangenbergStaff MemberORCiDGND |
Year of Publication: | 2012 |
Publisher: | Akadémiai Kiadó |
First Page: | 493 |
Last Page: | 497 |
Parent Title (English): | JPC : Journal of Planar Chromatography - Modern TLC |
Volume: | 25 |
Issue: | 6 |
ISSN: | 0933-4173 (Print) |
ISSN: | 1789-0993 (Online) |
DOI: | https://doi.org/10.1556/JPC.25.2012.6.1 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Tag: | 2D-TLC; Diode-array detection; HPTLC; Sulphonamides; TLC | Formale Angaben |
Open Access: | Closed |
Licence (German): | Urheberrechtlich geschützt |