Fluorescence Evaluation Using the Kubelka-Munk Formula
- A new formula is presented for transforming fluorescence measurements in accordance with Kubelka-Munk theory. The fluorescence signals, the absorption signals, and data from a selected reference are combined in one expression. Only diode-array techniques can measure all the required data simultaneously to linearize fluorescence data correctly. To prove the new theory HPTLC quantification of theA new formula is presented for transforming fluorescence measurements in accordance with Kubelka-Munk theory. The fluorescence signals, the absorption signals, and data from a selected reference are combined in one expression. Only diode-array techniques can measure all the required data simultaneously to linearize fluorescence data correctly. To prove the new theory HPTLC quantification of the analgesic flupirtine was performed over the mass range 300 to 5000 ng per spot. The fluorescence calibration curve was linear over the whole range. The transformation of fluorescence measurements into linear mass-dependent data extends the technique of in-situ fluorescence analysis to the high concentration range. It also extends Kubelka-Munk theory from absorption to fluorescence analysis. The results presented also emphasize the importance of Kubelka-Munk theory for in-situ measurements in scattering media, especially in planar chromatography.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/7288 | Bibliografische Angaben |
Title (English): | Fluorescence Evaluation Using the Kubelka-Munk Formula |
Conference: | Symposium ‘Planar Chromatography 2004’ (May 23-25, 2004 : Visegrád, Hungary) |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Marion Weyandt-Spangenberg |
Year of Publication: | 2004 |
Publisher: | Akadémiai Kiadó |
First Page: | 164 |
Last Page: | 168 |
Parent Title (English): | JPC : Journal of Planar Chromatography - Modern TLC |
Volume: | 17 |
Issue: | 3 |
ISSN: | 1789-0993 (Online) |
ISSN: | 0933-4173 (Print) |
DOI: | https://doi.org/10.1556/JPC.17.2004.3.1 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Tag: | Diode-array detector; Fluorescence measurements; Flupirtine; Kubelka-Munk theory | Formale Angaben |
Open Access: | Closed |
Licence (German): | Urheberrechtlich geschützt |